Patents by Inventor Kazuki Kumada

Kazuki Kumada has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Fluorescent separase activity sensor
    Patent number: 10174359
    Abstract: Problem to be Solved An object of the present invention is to provide a separase sensor for visualizing separase activity in a living cell. Another object is to develop a function analysis system of cell division using the separase sensor and further obtain a simple screening method for an anticancer agent. Solution The separase sensor of the present invention has two types of fluorescent proteins different in fluorescence wavelength at both ends of an amino acid sequence containing a separase cleavage site and a localization-targeting sequence targeting to a specific site within a cell. Since timing of activating separase and location of separase in a cell can be specified by use of the sensor, cell division can be analyzed and an anticancer agent can be screened.
    Type: Grant
    Filed: July 10, 2013
    Date of Patent: January 8, 2019
    Assignee: JAPANESE FOUNDATION FOR CANCER RESEARCH
    Inventors: Toru Hirota, Norihisa Shindo, Kazuki Kumada
  • FLUORESCENT PROBE FOR CELL OBSERVATION AND METHOD USING SAME
    Publication number: 20150140590
    Abstract: Problem to be Solved An object of the present invention is to provide a separase sensor for visualizing separase activity in a living cell. Another object is to develop a function analysis system of cell division using the separase sensor and further obtain a simple screening method for an anticancer agent. Solution The separase sensor of the present invention has two types of fluorescent proteins different in fluorescence wavelength at both ends of an amino acid sequence containing a separase cleavage site and a localization-targeting sequence targeting to a specific site within a cell. Since timing of activating separase and location of separase in a cell can be specified by use of the sensor, cell division can be analyzed and an anticancer agent can be screened.
    Type: Application
    Filed: July 10, 2013
    Publication date: May 21, 2015
    Inventors: Toru Hirota, Norihisa Shindo, Kazuki Kumada