Abstract: A biological sample holding container includes: a container main body having a bottom surface portion having a placement region for a biological sample and a side surface portion provided on the bottom surface portion so as to surround the placement region; and an annular holding member insertable and removable inside the side surface portion. The holding member holds a film covering the biological sample on the placement region with the bottom surface portion by being inserted inside the side surface portion together with the film.

Abstract: A fluorescence observation device is a device for performing fluorescence observation of a sample piece cut out from a sample including: a tray on which the sample piece is placed; a light source unit which generates excitation light to irradiate the sample piece; a detection unit which detects fluorescence from the sample piece; and an image generation unit which generates a fluorescence image of the sample piece based on a detection signal from the detection unit and the tray includes a plurality of placement regions provided around a center region of the tray and also includes a marker portion indicating a cutting orientation of the sample piece with respect to the sample.

Abstract: A fluorescence observation device is a device for performing fluorescence observation of a sample piece cut out from a sample including: a tray on which the sample piece is placed; a light source unit which generates excitation light to irradiate the sample piece; a detection unit which detects fluorescence from the sample piece; and an image generation unit which generates a fluorescence image of the sample piece based on a detection signal from the detection unit and the tray includes a plurality of placement regions provided around a center region of the tray and also includes a marker portion indicating a cutting orientation of the sample piece with respect to the sample.

Abstract: The purpose of the present invention is to provide a method for allowing a target cell to emit light, in which the target cell is present inside of a cell mass and is aimed to emit light by a fluorescent probe. The present invention provides a method for allowing the target cell to emit fluorescence comprising removing calcium ions from within the cell mass or a tissue containing the target cell and contacting the cell mass or the tissue with the fluorescent probe which emits fluorescence when contacted with or taken up into the target cell, thereby allowing the target cell present inside of the cell mass or the tissue to emit light, and thus can be used in a detection of the target cell in a sample, such as a clinical sample, in which the target cell may not be present on the surface of the sample.

Abstract: A fluorescent image acquisition system includes a light emitting device which emits excitation light during ON period and stop the emission of the excitation light during OFF period, an imaging device which outputs ON image data and OFF image data, an input device which sets a coefficient, an image processing unit which creates fluorescent image data and background image data, and an identification unit which identifies fluorescent pixels and non-fluorescent pixels. In order to create a superimposed image, the image processing unit calculates values obtained by summing pixel values of fluorescent pixels of the fluorescent image data and pixel values of fluorescent pixels of the background image data at a ratio of 1:1, as pixel values of the fluorescent pixels and calculates values obtained by multiplying at least pixel values of the non-fluorescent pixels of the background image by the coefficient, as pixel values of the non-fluorescent pixels.

Abstract: A fluorescence viewing device is configured to take a difference between one of an image of a first frame and an image of a second frame output from an imaging device and the other of the first frame image and the second frame image stored in an image storage means, whereby clear observed images can be obtained without influence of background light. In the fluorescence viewing device, an exposure time of a fluorescence image acquisition period and an exposure time of a background image acquisition period are different from each other. Since ON/OFF durations of excitation light also become asymmetric according to the asymmetry of the exposure times, the device can reduce user's feeling of strangeness due to blinking of the excitation light.

Abstract: A fluorescent image acquisition system includes a light emitting device which emits excitation light during ON period and stop the emission of the excitation light during OFF period, an imaging device which outputs ON image data and OFF image data, an input device which sets a coefficient, an image processing unit which creates fluorescent image data and background image data, and an identification unit which identifies fluorescent pixels and non-fluorescent pixels. In order to create a superimposed image, the image processing unit calculates values obtained by summing pixel values of fluorescent pixels of the fluorescent image data and pixel values of fluorescent pixels of the background image data at a ratio of 1:1, as pixel values of the fluorescent pixels and calculates values obtained by multiplying at least pixel values of the non-fluorescent pixels of the background image by the coefficient, as pixel values of the non-fluorescent pixels.

Abstract: A fluorescence image acquisition system is a system generating a fluorescence image by imaging fluorescence emitted from an observation object, the system including a light emitting device for iterating an output and stoppage of excitation light by emitting the excitation light toward the observation object during an ON period and stopping the emission of the excitation light during an OFF period, an imaging device having a light receiving unit including a plurality of pixels two-dimensionally arranged and an exposure control unit for controlling an exposure of the light receiving unit, and for outputting ON image data and OFF image data by capturing image of the observation object during each of the ON period and the OFF period, and a camera controller for generating fluorescence image data and setting an exposure time of the light receiving unit on the basis of the ON image data and the OFF image data.

Abstract: A fluorescence viewing device is configured to take a difference between one of an image of a first frame and an image of a second frame output from an imaging device and the other of the first frame image and the second frame image stored in an image storage means, whereby clear observed images can be obtained without influence of background light. In the fluorescence viewing device, an exposure time of a fluorescence image acquisition period and an exposure time of a background image acquisition period are different from each other. Since ON/OFF durations of excitation light also become asymmetric according to the asymmetry of the exposure times, the device can reduce user's feeling of strangeness due to blinking of the excitation light.