Abstract: A method of producing a volatile chemical substance uses a culture apparatus including: a culture tank configured to culture a microorganism having an ability to produce a volatile chemical substance; a delivery line configured to deliver a culture liquid obtained by culture in the culture tank to the storage tank; and a storage tank configured to store a solution containing the chemical substance obtained by culturing the microorganism, the method including culturing the microorganism in the culture tank while retaining the solution in the storage tank, wherein a gas phase portion in the storage tank and a gas phase portion in the culture tank are connected to each other through a gas connection pipe.

Abstract: A method produces an alcohol by continuous fermentation using a fermentation raw material, the method including: culturing a microorganism to obtain a fermented liquid, while supplying the fermentation raw material into a fermenter using a plurality of fermentation raw material supply systems; filtering the obtained fermented liquid through a separation membrane to obtain an alcohol-containing filtered liquid and an unfiltered liquid; and retaining or refluxing the obtained unfiltered liquid in the fermenter, in which the plurality of fermentation raw material supply systems each include a fermentation raw material tank for storing the fermentation raw material the same as each other, and a supply line connecting the fermentation raw material tank and the fermenter; and selecting a fermentation raw material supply system to be allowed to stop supplying the fermentation raw material in rotation from the plurality of fermentation raw material supply systems during the supply of the fermentation raw material.

Abstract: A lactic acid-producing yeast mutant which is a mutant of a yeast prepared by introduction of a lactate dehydrogenase gene that results in lactic acid-producing ability, the mutant having, in a medium at pH 3, a lactic acid-producing ability equivalent to or higher than the lactic acid-producing ability of a parent strain of the mutant at a lactic acid fermentation optimum pH.

Abstract: By a method for producing cadaverine by culturing a microorganism that extracellularly secretes lysine decarboxylase, by-production of lysine is suppressed, the yield of cadaverine relative to glucose consumption is improved compared to conventional production methods, and further, the load on the purification step in purification of cadaverine as a raw material for polyamide can be reduced.

Abstract: Highly productive D-lactic acid fermentation uses a transformant obtained by introducing into a host cell a polynucleotide encoding a polypeptide according to any one of the following (A) to (C) in such a manner that the polypeptide is expressed, which polypeptide has a D-lactate dehydrogenase activity higher than those of conventional polypeptides: (A) a polypeptide having the amino acid sequence shown in SEQ ID NO:1 or 2; (B) a polypeptide having the same amino acid sequence as shown in SEQ ID NO:1 or 2 except that one or several amino acids are substituted, deleted, inserted and/or added, which polypeptide has a D-lactate dehydrogenase activity; and (C) a polypeptide having an amino acid sequence which has a sequence identity of not less than 80% to the amino acid sequence shown in SEQ ID NO:1 or 2, which polypeptide has a D-lactate dehydrogenase activity.

Abstract: A lactic acid-producing yeast mutant which is a mutant of a yeast prepared by introduction of a lactate dehydrogenase gene that results in lactic acid-producing ability, the mutant having, in a medium at pH 3, a lactic acid-producing ability equivalent to or higher than the lactic acid-producing ability of a parent strain of the mutant at a lactic acid fermentation optimum pH.

Abstract: By a method for producing cadaverine by culturing a microorganism that extracellularly secretes lysine decarboxylase, by-production of lysine is suppressed, the yield of cadaverine relative to glucose consumption is improved compared to conventional production methods, and further, the load on the purification step in purification of cadaverine as a raw material for polyamide can be reduced.

Abstract: Highly productive D-lactic acid fermentation uses a transformant obtained by introducing into a host cell a polynucleotide encoding a polypeptide according to any one of the following (A) to (C) in such a manner that the polypeptide is expressed, which polypeptide has a D-lactate dehydrogenase activity higher than those of conventional polypeptides: (A) a polypeptide having the amino acid sequence shown in SEQ ID NO:1 or 2; (B) a polypeptide having the same amino acid sequence as shown in SEQ ID NO:1 or 2 except that one or several amino acids are substituted, deleted, inserted and/or added, which polypeptide has a D-lactate dehydrogenase activity; and (C) a polypeptide having an amino acid sequence which has a sequence identity of not less than 80% to the amino acid sequence shown in SEQ ID NO:1 or 2, which polypeptide has a D-lactate dehydrogenase activity.