Abstract: A method of manufacturing a surface-coated cutting tool includes: forming an aluminum oxide layer having a layer thickness of 0.05 to 5 ?m and an ?-alumina structure with a corundum type crystal structure on a cutting tool body using a sol-gel method. The step of forming includes adding an alcohol to aluminum alkoxide; adding an acid; stirring the mixture at 10° C. or lower to form a sol; applying the sol on a surface of the cutting tool body or an outer-most surface of a hard-coating layer formed on the surface of the cutting tool body; performing a drying process at least once, the applied sol being dried at 100 to 400° C. in the drying process; and annealing the cutting tool body with a dried sol layer at 500 to 1000° C.

Abstract: An object of the present invention is to provide a plant having environmental stress resistance. The present invention provides a transgenic plant having environmental stress resistance, in which an exogenous DNA encoding a transcription factor selected from the group consisting of transcription factors from Arabidopsis thaliana TCP13, TCP5 and TCP17, and homologous transcription factors thereof from other plants is overexpressed, and a method for producing such a plant.

Abstract: The present invention relates to a transgenic plant tolerant to environmental stress that comprises DNA encoding an exogenous abscisic acid (ABA) transporter protein in an expressible manner, a progeny thereof, or a cell, tissue or seed from such plant. The present invention also relates to a method for producing such a plant.

Abstract: A method of manufacturing a surface-coated cutting tool includes: forming an aluminum oxide layer having a layer thickness of 0.05 to 5 ?m and an ?-alumina structure with a corundum type crystal structure on a cutting tool body using a sol-gel method. The step of forming includes adding an alcohol to aluminum alkoxide; adding an acid; stirring the mixture at 10° C. or lower to form a sol; applying the sol on a surface of the cutting tool body or an outer-most surface of a hard-coating layer formed on the surface of the cutting tool body; performing a drying process at least once, the applied sol being dried at 100 to 400° C. in the drying process; and annealing the cutting tool body with a dried sol layer at 500 to 1000° C.

Abstract: The present invention enables production of a plant resistant to environmental stresses including drought stress. The invention increases stress resistance to a plant by increasing raffinose content in the plant.

Abstract: This invention concerns induction of gene expression specifically in given tissue with the use of a novel promoter that has environmental stress responsiveness and functions specifically in given tissue.

Abstract: A gene causative of a phenotype is easily specified and thus gene functions are comprehensively analyzed. A method for analyzing a gene function includes (a) infecting a plant group with a full-length cDNA library containing an expression regulatory sequence to introduce the cDNA into the plants; (b) selecting a plant complying with desired selection requirements from the plants into which the cDNA has been introduced; (c) isolating the cDNA from the selected plant; and (d) re-introducing the thus isolated cDNA into a plant of the same species as the plant into which the cDNA has been introduced, to reconfirm a phenotype according to the selection requirements.

Abstract: A DNA encoding a neoxanthin cleavage enzyme used for improving stress tolerance in a plant, a method for increasing stress tolerance in a plant by introducing the DNA into the plant, and a transgenic plant into which a neoxanthin cleavage enzyme gene is introduced, are provided. A DNA used for reducing stress tolerance in a plant, a method for decreasing stress tolerance in a plant by introducing the DNA into the plant, and a transgenic plant into which the DNA is introduced, are also provided. The present invention enables creating a plant in which stress tolerance has been increased or decreased.

Abstract: The present invention provides a method of regulating expression of a gene by (a) preparing a recombinant plant cell line, plant tissue or plant with an expression vector having an abiotic environmental stress-responsive promoter of SEQ ID NO: 27 and the gene downstream thereof; and (b) culturing and cultivating the recombinant plant cell, plant tissue or plant under an abiotic environmental stress, wherein the promoter regulates the expression of the gene under the abiotic environmental stress.

Abstract: The present invention provides a method of regulating expression of a gene by (a) preparing a recombinant plant cell line, plant tissue or plant with an expression vector having an abiotic environmental stress-responsive promoter of SEQ ID NO: 27 and the gene downstream thereof; and (b) culturing and cultivating the recombinant plant cell, plant tissue or plant under an abiotic environmental stress, wherein the promoter regulates the expression of the gene under the abiotic environmental stress.

Abstract: This invention provides a stress tolerant transgenic plant with the utilization of a signal transduction factor located upstream of the gene group associated with environmental responses. This invention provides a gene encoding the following protein (a) or (b) and a stress tolerant transgenic plant to which such gene has been introduced: (a) a protein consisting of the amino acid sequence as shown in SEQ ID NO: 2 in the Sequence Listing; or (b) a protein consisting of an amino acid sequence derived from the amino acid sequence as shown in SEQ ID NO: 2 in the Sequence Listing by deletion. substitution, or addition of one or several amino acid residues and having activity of inducing expression of stress responsive transcription factors and activity of protein kinase.

Abstract: A stress-tolerant transgenic plant comprising an exogenously-introduced polynucleotide encoding a protein kinase which activates stress-responsive transcription factors. The polynucleotide may encode a protein kinase which comprises SEQ ID NO: 2 (SRK-2C) or a sequence having a significant degree of similarity with SEQ ID NO: 2. The transgenic plant has increased stress-tolerance to environmental stresses, such as to dehydration, osmotic stress or low temperature stress, compared to a similar non-transgenic plant.

Abstract: The environmental stress responsive promoter of the present invention comprises DNA of the following (a), (b) or (c): DNA consisting of any nucleotide sequence selected from SEQ ID NOS: 1 to 90; DNA consisting of a nucleotide sequence comprising a deletion, substitution or addition of one or more nucleotides relative to any nucleotide sequence selected from SEQ ID NOS: 1 to 90, and functioning as an environmental stress responsive promoter; and (c) DNA hybridizing under stringent conditions to DNA consisting of any nucleotide sequence selected from SEQ ID NOS: 1 to 90, and functioning as an environmental stress responsive promoter.

Abstract: The present invention provides a stress responsive promoter. The environmental stress responsive promoter of the present invention comprises DNA of the following (a), (b) or (c): (a) DNA consisting of any nucleotide sequence selected from SEQ ID NOS: 1 to 18; (b) DNA consisting of a nucleotide sequence comprising a deletion, substitution or addition of one or more nucleotides relative to any nucleotide sequence selected from SEQ ID NOS: 1 to 18, and functioning as an environmental stress responsive promoter; and (c) DNA hybridizing under stringent conditions to DNA consisting of any nucleotide sequence selected from SEQ ID NOS: 1 to 18, and functioning as an environmental stress responsive promoter.

Abstract: A DNA encoding a neoxanthin cleavage enzyme used for improving stress tolerance in a plant, a method for increasing stress tolerance in a plant by introducing the DNA into the plant, and a transgenic plant into which a neoxanthin cleavage enzyme gene is introduced, are provided. A DNA used for reducing stress tolerance in a plant, a method for decreasing stress tolerance in a plant by introducing the DNA into the plant, and a transgenic plant into which the DNA is introduced, are also provided. The present invention enables creating a plant in which stress tolerance has been increased or decreased.

Abstract: The present invention enables production of a plant resistant to environmental stresses including drought stress. The invention increases stress resistance to a plant by increasing raffinose content in the plant.

Abstract: The present invention enables production of a plant resistant to environmental stresses including drought stress. The invention increases stress resistance to a plant by increasing raffinose content in the plant.

Abstract: A DNA encoding a neoxanthin cleavage enzyme used for improving stress tolerance in a plant, a method for increasing stress tolerance in a plant by introducing the DNA into the plant, and a transgenic plant into which a neoxanthin cleavage enzyme gene is introduced, are provided. A DNA used for reducing stress tolerance in a plant, a method for decreasing stress tolerance in a plant by introducing the DNA into the plant, and a transgenic plant into which the DNA is introduced, are also provided. The present invention enables creating a plant in which stress tolerance has been increased or decreased.

Abstract: The present invention provides a novel NMR metabolomics method for observing an animal by labeling the animal itself. A labeled animal labeled with a stable isotope is produced by feeding an animal a labeled food labeled with the stable isotope. Metabolism of a biological substance in the animal is analyzed based on nuclear magnetic resonance data of the biological substance containing the stable isotope. The nuclear magnetic resonance data is acquired by performing NMR measurement on a body of the labeled animal, a portion of the body, or an extract.

Abstract: The present invention provides a method of analyzing metabolism of a plant in which measurement sensitivity for nuclides such as 13C and 15N having low natural abundance ratios is improved using NMR. A labeled plant uniformly labeled with a stable isotope is produced by providing the plant with a nutrition source labeled with at least one type of stable isotope while growing the plant from a seed or a germinating seedling. The metabolism of a biological substance in the plant is analyzed based on NMR data of a biological substance that contains the stable isotope. The NMR data is acquired by performing NMR measurement on a body of the labeled plant, a portion of the body, or an extract.