Abstract: A fluid pipetting method is to pipette and distribute a fluid into a plurality of vessels by means of a single probe. In pipetting operations, a cleaning liquid, an air layer and the total sum of the fluid including a part of surplus, the total of the fluid to be pipetted and a part of waste are picked up into the probe in the order listed and an amount of pipetting the fluid into respective vessels is controlled in accordance with the pipetting order in such a manner that the amount of the fluid pipetted and distributed into each of vessels is substantially a given value.