Abstract: The present disclosure provides for an expression vector comprising (i) at least one nucleic acid sequence encoding a transcriptional gene silencing element; and (ii) at least two other nucleic acid sequences selected from the group consisting of a nucleic acid sequence that encodes a nucleic acid molecule that inhibits an HIV co-receptor; a nucleic acid sequence that encodes an HIV fusion inhibitor protein; a nucleic acid sequence encoding an inhibitor of HIV replication; and a nucleic acid sequence encoding an inhibitor of viral entry. The combination of the three mechanisms in a single expression vector provides for novel means of inhibiting HIV-I.

Abstract: Provided is an inkjet printing apparatus capable of performing preliminary discharge without the influence of mist while suppressing degradation in throughput regardless of a preliminary discharge time. Specifically, an inkjet printing apparatus that performs a wiping operation on a nozzle arrangement surface provided with nozzles discharging an ink in a print head includes a wiping determination unit that determines whether to perform the wiping operation before preliminary discharge based on the amount of the preliminary discharge to be performed.

Abstract: The present disclosure provides compositions (i.e., amplification primers and probes), methods, and kits that are particularly useful for detecting and/or quantifying nucleic acids present in a sample, such as those derived from HIV or a lentiviral vector.

Abstract: A recording device including a recording head, a tank, a valve capable of being switched between an open state where ink is supplied from the tank to the recording head and a closed state where the ink is not supplied, a cap covering a discharge port surface, a pump configured to make a pressure of an inside of the cap negative, an acquiring unit configured to acquire information on an atmospheric pressure of an environment where the recording device is placed, and a control unit configured to perform a suction operation of sucking the ink from the discharge port surface by driving the pump when the valve is in the closed state, making the pressure of the inside of the cap negative, and then switching the valve to the open state and to control the suction operation based on the acquired information.

Abstract: Provided is an inkjet printing apparatus capable of performing preliminary discharge without the influence of mist while suppressing degradation in throughput regardless of a preliminary discharge time. Specifically, an inkjet printing apparatus that performs a wiping operation on a nozzle arrangement surface provided with nozzles discharging an ink in a print head includes a wiping determination unit that determines whether to perform the wiping operation before preliminary discharge based on the amount of the preliminary discharge to be performed.

Abstract: An inkjet printing apparatus includes a head, a platen, a platen absorbent that temporarily stores ink ejected from the head, a waste ink storage container that stores the ink discharged from the platen absorbent, and an estimation unit that, in a case where the platen is divided into a plurality of regions in a direction intersecting a conveyance direction of the printing medium, estimates an amount of ink stored in the waste ink storage container based on a position of a region to which the ink is ejected by the head and an amount of ink ejected to the region.

Abstract: An inkjet printing apparatus capable of detecting an ink amount in an attachable inktank at a multi-level with a simple arrangement has the following arrangement. That is, the printing apparatus includes a printhead which prints an image by discharging ink, an inktank which contains the ink supplied to the printhead, a reservoir capable of temporarily containing the ink supplied from the inktank, and a detection unit which performs a detection operation of detecting whether the ink amount in the inktank is more than a predetermined amount. Then, the detection unit performs the detection operation in a state in which the reservoir contains the ink supplied from the inktank.

Abstract: Described herein are silencing nucleic acids, compositions comprising silencing nucleic acids, and methods of utilizing the silencing nucleic acids to to inhibit HIV replication in a cell infected with HIV. In some embodiments, the disclosed methods comprise contacting a cell or population of cells infected with HIV with at least one silencing nucleic acid that targets a specific sequence of the 5? LTR of HIV.

Abstract: An inkjet printing apparatus includes a printhead, a subtank for storing ink to be supplied to the printhead, a maintank for storing ink to be supplied to the subtank, a valve that can be switched between an open state in which the printhead communicates with the subtank and a closed state in which the printhead does not communicate with the subtank, a cap for covering an discharging port surface of the printhead, a pump for generating a negative pressure in an inside of the cap with the cap covering the discharging port surface, and an internal pressure changing member for changing an internal pressure of the subtank to perform a subtank filling operation in which ink is supplied from maintank to the subtank.

Abstract: An inkjet recording apparatus includes a first ink tank that contains ink, a second ink tank that contains ink supplied from the first ink tank, a communication unit, having conductivity, communicates between the first ink tank and the second ink tank, an opening provided at one end of the communication unit connected to the second ink tank and opening to the second ink tank, an electrode portion provided in the second ink tank, of which a lower end position is located at or above a lower end position of the one end of the communication unit and at or below a highest position of the opening, and a detecting unit that detects an ink amount in the second ink tank in accordance with electrical properties between the electrode portion and the communication unit.

Abstract: According to the present invention, a packing member (20) is disposed between a mouth portion (2b) of a container body (2) and a lid base portion (5). A crushing protrusion (20b) which protrudes downward and is crushed to an upper end opening edge of the container body (2) is formed on a lower surface of the packing member (20). An overhang recess (20c) which is at least partially located just above the crushing protrusion (20b) is formed on an upper surface of the packing member (20). After the lid base portion (5), the movable lid portion (6) and the syringe tube (8) are detached from the container body (2), when mounting again, until a crushing protrusion (20b) is crushed, the packing member (20) forms a communication gap (30) between the mouth portion (2b) of the container body (2) and the packing member (20).

Abstract: A printing apparatus performs a printing operation where mist that is generated in inkjet printing is efficiently collected. Specifically, a fan is rotated during a mist collection operation at a maintenance operation with a rotating speed that is greater than the rotating speed of the fan during printing.

Abstract: An ink jet recording apparatus includes a recording head ejecting ink; a buffer tank temporarily storing the ink; a mounting unit to which a main tank that stores the ink is capable of being mounted, the main tank having a first supply port from which the ink is supplied to the recording head and a second supply port from which the ink is supplied to the buffer tank; a first detecting unit detecting an ink volume in the main tank; and a control unit performing a discharge operation to discharge the ink in the buffer tank from the recording head via the main tank in a pre-transportation process in the ink jet recording apparatus. The control unit prompts a user to replace the main tank without performing the discharge operation if the ink volume detected by the first detecting unit is smaller than a threshold value.

Abstract: A printer according to this invention includes a print head, a carriage, a recovery unit, and a controller. The controller causes a recovery operation to be performed based on a total value of two values. Assuming that front and rear arrays of discharge port arrays during outbound movement of the carriage are defined as first and second arrays, respectively, the first value is obtained by multiplying a sum of an amount of ink discharged from the first array during outbound movement of the carriage and an amount of ink discharged from the second array during homebound movement of the carriage by a first coefficient, and the second value is obtained by multiplying a sum of an amount of ink discharged from the second array during outbound movement of the carriage and an amount of ink discharged from the first array during homebound movement of the carriage by a second coefficient.

Abstract: In a printer in which an inkjet head is mounted in a carriage, which is movable in a direction intersecting with a conveyance direction of a recording medium, and ink is ejected from the inkjet head to record an image onto the recording medium, there has been a problem in that the recording medium is charged due to friction and separation at the time of conveyance of the recording medium, and ink mist is attracted to a portion charged to have an unexpected pattern to be recorded. In view of this problem, static electricity generated on the recording medium is removed by providing the carriage with an ionizer for generating a positive ion and an ionizer for generating a negative ion and generating the ions at the time of scanning of the carriage. The static electricity can be removed efficiently by arranging on the carriage the ionizers for generating the ions of both polarities, namely, the positive ions and the negative ions.

Abstract: A recording apparatus includes a recording head, a sub tank, an ink tank containing ink supplied to the sub tank, a detector that detects an amount of ink contained in the sub tank, a variable volume member provided between the recording head and the sub tank and having variable inner volume, and a controller that repeatedly performs first and second operations, thereby supplying ink from the ink tank to the sub tank. In the first operation, the member is caused to switch from a first state to a second state, while in the second operation, the member is caused to switch from the second state to the first state.

Abstract: An ink jet recording apparatus is capable of suppressing an increase of waste ink while solving an increase in density of ink within a supply tube. An ink jet recording apparatus according to the present invention includes a recording head configured to discharge ink, a tank configured to store ink, a supply tube configured to supply ink to the recording head from the tank, a discharge unit configured to execute a discharge operation for discharging ink within the supply tuber a control unit configured to make the discharge unit execute a discharge operation when an elapsed time from a previous discharge operation is greater than a predetermined time, and a changing unit configured to change at least any one of the elapsed time or the predetermined time based on an ink consumption amount consumed through the recording head after the previous discharge operation.

Abstract: A recording apparatus includes a recording head, a sub tank, an ink tank containing ink supplied to the sub tank, a detector that detects an amount of ink contained in the sub tank, a variable volume member provided between the recording head and the sub tank and having variable inner volume, and a controller that repeatedly performs first and second operations, thereby supplying ink from the ink tank to the sub tank. In the first operation, the member is caused to switch from a first state to a second state, while in the second operation, the member is caused to switch from the second state to the first state.

Abstract: Provided is an inkjet printing apparatus capable of performing preliminary discharge without the influence of mist while suppressing degradation in throughput regardless of a preliminary discharge time. Specifically, an inkjet printing apparatus that performs a wiping operation on a nozzle arrangement surface provided with nozzles discharging an ink in a print head includes a wiping determination unit that determines whether to perform the wiping operation before preliminary discharge based on the amount of the preliminary discharge to be performed.

Abstract: An inkjet printing apparatus includes a printhead, a subtank for storing ink to be supplied to the printhead, a maintank for storing ink to be supplied to the subtank, a valve that can be switched between an open state in which the printhead communicates with the subtank and a closed state in which the printhead does not communicate with the subtank, a cap for covering an discharging port surface of the printhead, a pump for generating a negative pressure in an inside of the cap with the cap covering the discharging port surface, and an internal pressure changing member for changing an internal pressure of the subtank to perform a subtank filling operation in which ink is supplied from maintank to the subtank.