Abstract: It is made possible to quantify MT1-MMP and to measure the enzymatic activity of MT1-MMP sensitively, accurately and quickly with simple operations and reagents. The MT1-MMP quantifications and the MT1-MMP enzymatic activity measurements can be carried out using quantitative immunoassays for MT1-MMP with anti-MT1-MMP Ab and reagents used therefor, further immunologically quantifying methods for a member selected from (i) MT1-MMP released and/or solubilized from cell membrane with a surfactant and/or a reducing agent and (ii) spontaneously solubilized MT1-MMP, reagents used therefor, solid-phased MT1-MMP, etc., thereby enabling the screening for a compound which promotes or inhibits the expression of MT1-MMP or a compound which promotes or inhibits the enzymatic activity of MT1-MMP, and facilitating the development and research for valuable pharmaceutical drugs. There are also provided a useful testing drug for cancer or cancer metastasis.

Abstract: A device for arranging conductive particles in a preselected pattern for the connection of electric circuit boards or electric parts is disclosed. Particularly, a device capable of surely and efficiently transferring, e.g., solder bumps to the electrode pads of a semiconductor chip or the leads of a TAB (Tape Automated Bonding) tape and a conductive particle transferring method using the same are disclosed.

Abstract: A process for manufacturing a device for arranging conductive particles in a preselected pattern for the connection of electric circuit boards or electric parts is disclosed. Particularly, a device capable of surely and efficiently transferring, e.g., solder bumps to the electrode pads of a semiconductor chip or the leads of a TAB (Tape Automated Bonding) tape and a conductive particle transferring method using the same are disclosed.

Abstract: Anti-human stromelysin monoclonal antibodies reactive with latent and active forms of stromelysin without discrimination between the two, each being immunoreactive with only one of the antigenic determinants of human stromelysin, are provided. The use of a combination of two such monoclonal antibodies which specifically react with different antigenic determinants of human stromelysin renders it possible to accurately determine the amount of human stromelysin in human body fluids, and thus to carry out the diagnosis of rheumatoid arthritis.There are thus provided said monoclonal antibodies per se, a sandwich enzyme immunoassay for the determination of the amount of human stromelysin in human body fluid samples using a combination of two such monoclonal antibodies, and a method for the diagnosis of rheumatoid arthritis using said immunoassay.

Abstract: Provided are serum-free media for tissue culture containing tissue inhibitor of metalloproteinases and methods for culturing animal cells using these media.

Abstract: A method for determining human type III, IV and VI collagen peptides by way of enzyme immunoassay according to the sandwich technique using a monoclonal antibody and a polyclonal antibody each to the human type III, IV or VI collagen peptide, characterized in that the monoclonal antibody to the collagen peptide is used as an antibody to be coated onto a solid phase and/or an antibody to be labeled with an enzyme. Also disclosed is a sandwich technique using two different monoclonal antibodies. This method can be carried out with a smaller amount of samples in a simple manner to obtain a precise result and is thus useful for diagnosis of hepatic diseases.

Abstract: A method for diagnosing rheumatoid arthritis, which is characterized by enzyme-immunologically measuring the amount of collagenase inhibitor present in sera, plasmas or synovial fluids by way of a sandwich assay wherein two different monoclonal antibodies which specifically bind to different antigenic determinants of the collagenase inhibitor are used, and comparing the measured amount with that for normal subjects.

Abstract: A method for diagnosing hepatic carcinoma, which is characterized by enzyme-immunologically measuring the amount of collagenase inhibitor present in sera, plasmas of synovial fluids by way of a sandwich assay wherein two different monoclonal antibodies which specifically bind to different antigenic determinants of the collagenase inhibitor are used, and comparing the measured amount with that for normal subjects.

Abstract: The present invention is directed to a method for detecting hepatic diseases which are associated with fibrosis by determining the level of human prolyl hydroxylase in a serum sample which comprises:(a) contacting a serum sample of a patient suspected of having said hepatic disease associated with fibrosis with a monoclonal antibody specific to the .beta.-subunit of human prolyl hydroxylase to form an antigen antibody complex bound on a solid support;(b) contacting said antigen antibody complex bound on said solid support with an enzyme-labeled monoclonal or enzyme-labeled polyclonal antibody specific to human prolyl hydroxylase to form an antibody antigen enzyme-labeled antibody complex; and(c) measuring the amount of enzyme activity of said bound antibody antigen enzyme-labeled antibody complex to determine the level of human prolyl hydroxylase present in said serum sample.

Abstract: A method for determining human prolyl hydroxylase by radioimmunoassay according to the sandwich method wherein a monoclonal antibody to human prolyl hydroxylase and polyclonal antibody to human prolyl hydroxylase are used, characterized in that a monoclonal antibody to human prolyl hydroxylase is used as at least one of the antibodies which are to be coated on a solid support and to be labeled with a radioactive element. This method is simple and operable with small amounts of samples and gives exact results. Thus, this method is useful for the diagnosis of hepatic diseases. A monoclonal antibody specific to the .beta.-subunit of human prolyl hydroxylase is used to test for the human prolyl hydroxylase.