Abstract: By using a mutant glucose oxidase comprising an amino acid sequence in which a residue corresponding to isoleucine at position 489 or arginine at position 335 in the amino acid sequence of SEQ ID NO:1 is substituted with an amino acid residue having a reactive functional group in a side chain, and binding an electron acceptor to the mutant glucose oxidase through the amino acid residue having a reactive functional group, an electron acceptor-modified glucose oxidase is obtained.

Abstract: A mutant cytochrome protein originated from a cytochrome protein having three heme-binding domains, which mutant cytochrome protein lacks the first heme-binding domain and the second heme-binding domain as counted from the N-terminus, is provided. The mutant cytochrome protein may lack a region(s) containing the first and second heme-binding domains.

Abstract: Provided is a construction machine capable of driving each hydraulic actuator at a suitable speed while suppressing delivery flow rate of a hydraulic pump, both at a single operation time of driving each hydraulic actuator respectively singularly and at a combined operation time of simultaneously driving a plurality of hydraulic actuators.

Abstract: A hydraulic drive system for a hydraulic excavator that enables leveling work and a jack-up operation by a blade in a float state, that can prevent a body from falling even when an operator has falsely operated the hydraulic excavator during the jack-up operation by the blade, and that yet can perform favorable leveling work with the blade turned into the float state is provided. A float switch 37, a float valve 38, and a controller 34 are provided, the float valve 38 is changed over to a float position VI upon operating the float switch 37 when the blade is not in a jack-up state, the float valve 38 is changed over from the float position VI to a normal position V when the float valve 38 is in a state of being at the float position VI and an operation lever device 22 has been operated, and the float valve 38 is kept at the normal position V when the float valve 38 is at the normal position V and the float switch 37 has been operated in the jack-up state.

Abstract: To provide a construction machine that can improve the energy efficiency in a leveling operation in which an arm crowding operation and a boom raising operation are performed simultaneously.

Abstract: Provided is a construction machine capable of driving each hydraulic actuator at a suitable speed while suppressing delivery flow rate of a hydraulic pump, both at a single operation time of driving each hydraulic actuator respectively singularly and at a combined operation time of simultaneously driving a plurality of hydraulic actuators.

Abstract: To provide a construction machine that can improve the energy efficiency in a leveling operation in which an arm crowding operation and a boom raising operation are performed simultaneously.

Abstract: To provide a small hydraulic excavator, which even may have an upperstructure formed such that at least its rear end swings within a body width range and having a limited installation space for devices, that allows an accumulator to be disposed and allows the accumulator to be protected from external force generated during work. The present invention relates to a rear small-swing type mini excavator that includes an accumulator 30 accommodating and recovering potential energy and hydraulic energy used by at least one of drives of an undercarriage 1, an upperstructure 2 formed such that its rear end swings within the body width range of the undercarriage 1, and a working device 3.

Abstract: A mutant cytochrome protein originated from a cytochrome protein having three heme-binding domains, which mutant cytochrome protein lacks the first heme-binding domain and the second heme-binding domain as counted from the N-terminus, is provided. The mutant cytochrome protein may lack a region(s) containing the first and second heme-binding domains.

Abstract: A mutant cytochrome protein originated from a cytochrome protein having three heme-binding domains, which mutant cytochrome protein lacks the first heme-binding domain and the second heme-binding domain as counted from the N-terminus, is provided. The mutant cytochrome protein may lack a region(s) containing the first and second heme-binding domains.

Abstract: By using a mutant glucose oxidase comprising an amino acid sequence in which a residue corresponding to isoleucine at position 489 or arginine at position 335 in the amino acid sequence of SEQ ID NO:1 is substituted with an amino acid residue having a reactive functional group in a side chain, and binding an electron acceptor to the mutant glucose oxidase through the amino acid residue having a reactive functional group, an electron acceptor-modified glucose oxidase is obtained.

Abstract: The purpose of the present invention is to provide a fungus-derived FADGDH that has a direct electron transfer ability. Provided is a fusion protein comprising a fungus-derived FADGDH or a mutant thereof and a cytochrome molecule connected to the N-terminus of the FADGDH or variant thereof.

Abstract: By using a mutant glucose oxidase comprising an amino acid sequence in which a residue corresponding to isoleucine at position 489 or arginine at position 335 in the amino acid sequence of SEQ ID NO:1 is substituted with an amino acid residue having a reactive functional group in a side chain, and binding an electron acceptor to the mutant glucose oxidase through the amino acid residue having a reactive functional group, an electron acceptor-modified glucose oxidase is obtained.

Abstract: A hydraulic drive system for a hydraulic excavator that enables leveling work and a jack-up operation by a blade in a float state, that can prevent a body from falling even when an operator has falsely operated the hydraulic excavator during the jack-up operation by the blade, and that yet can perform favorable leveling work with the blade turned into the float state is provided. A float switch 37, a float valve 38, and a controller 34 are provided, the float valve 38 is changed over to a float position VI upon operating the float switch 37 when the blade is not in a jack-up state, the float valve 38 is changed over from the float position VI to a normal position V when the float valve 38 is in a state of being at the float position VI and an operation lever device 22 has been operated, and the float valve 38 is kept at the normal position V when the float valve 38 is at the normal position V and the float switch 37 has been operated in the jack-up state.

Abstract: In a hydraulic drive system of a work machine which drives a plurality of actuators using three or more pumps, for an operation not including traveling, a highly efficient combined operation in a front implement and excellent combined operability of a swing and the front implement are enabled, while for an operation including traveling, a highly efficient traveling operation and a highly efficient combined operation of traveling and the front implement are enabled, and a sufficient operation speed of the front implement is achieved. To this end, each of the flow rates of the first, second, and third pumps (101, 201, 301) can be controlled independently by performing the load sensing control, and in a combined operation for driving a boom (511) and an arm (512), ether one of them is driven by the first pump while the other one is driven by the second pump, and the swing is driven by the third pump.

Abstract: To provide a small hydraulic excavator, which even may have an upperstructure formed such that at least its rear end swings within a body width range and having a limited installation space for devices, that allows an accumulator to be disposed and allows the accumulator to be protected from external force generated during work. The present invention relates to a rear small-swing type mini excavator that includes an accumulator 30 accommodating and recovering potential energy and hydraulic energy used by at least one of drives of an undercarriage 1, an upperstructure 2 formed such that its rear end swings within the body width range of the undercarriage 1, and a working device 3.

Abstract: Pressure compensating valves not fully closing at the stroke end are employed, and upon the operator's operation for the traveling, pilot primary pressure is reduced and supplied to remote control valves 34c-34h of non-travel operating devices. Thus, the inflow of the hydraulic fluid into non-travel actuators is suppressed and a necessary amount of hydraulic fluid for travel motors is secured in travel combined operation. Accordingly, when saturation occurs in a construction machine's hydraulic drive system performing the load sensing control due to combined operation with a great load pressure difference between two actuators, deceleration/stoppage of an actuator on the low load pressure side is prevented by preventing full closure of the pressure compensating valve on the low load pressure side, while also preventing deceleration/stoppage of a high load pressure actuator by securing a necessary amount of hydraulic fluid for the high load pressure actuator.

Abstract: By using a mutant glucose oxidase comprising an amino acid sequence in which a residue corresponding to isoleucine at position 489 or arginine at position 335 in the amino acid sequence of SEQ ID NO:1 is substituted with an amino acid residue having a reactive functional group in a side chain, and binding an electron acceptor to the mutant glucose oxidase through the amino acid residue having a reactive functional group, an electron acceptor-modified glucose oxidase is obtained.

Abstract: The present invention provides a mutant-type glucose dehydrogenase having glucose dehydrogenase activity and having decreased reactivity with xylose, wherein said mutant-type glucose dehydrogenase comprises a mutant-type ?-subunit comprising an amino acid sequence of 520 to 550 amino acids comprising an amino acid sequence of 520 to 550 amino acids comprising SEQ ID NO: 21, SEQ ID NO: 22, SEQ ID NO: 23, SEQ ID NO: 24 and SEQ ID NO: 25 in this order from N-terminus to C-terminus, except that one or more amino acid residue(s) selected from the group consisting of the glycine at position 10 in SEQ ID NO: 23, the histidine at position 4 in SEQ ID NO: 24, and the asparagine at position 4 in SEQ ID NO: 25 is/are substituted with another/other amino acid(s).

Abstract: In a hydraulic drive system of a work machine which drives a plurality of actuators using three or more pumps, for an operation not including traveling, a highly efficient combined operation in a front implement and excellent combined operability of a swing and the front implement are enabled, while for an operation including traveling, a highly efficient traveling operation and a highly efficient combined operation of traveling and the front implement are enabled, and a sufficient operation speed of the front implement is achieved. To this end, each of the flow rates of the first, second, and third pumps (101, 201, 301) can be controlled independently by performing the load sensing control, and in a combined operation for driving a boom (511) and an arm (512), ether one of them is driven by the first pump while the other one is driven by the second pump, and the swing is driven by the third pump.