Patents by Inventor Kefen WANG

Kefen WANG has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Alkaline protease mutant, and gene, engineered strain, preparation method and application thereof
    Patent number: 11655464
    Abstract: An alkaline protease mutant, and a gene, engineered strain, a preparation method and application thereof are provided. The method comprises the following steps of extracting genome DNA of Bacillus clausii, performing PCR amplification to obtain a wild-type alkaline protease gene sequence, mutating the wild-type alkaline protease gene obtained by the amplification through an error-prone PCR, performing high-throughput screening to obtain a plurality of highly active alkaline protease genes, performing DNA shuffling on the highly active alkaline protease genes, and performing screening to obtain eight alkaline protease mutant genes with higher activity.
    Type: Grant
    Filed: July 31, 2022
    Date of Patent: May 23, 2023
    Assignees: Tianjin University of Science and Technology, Shandong Lonct Enzymes Co. LTD
    Inventors: Fuping Lu, Yihan Liu, Yu Li, Xingji Wang, Kefen Wang, Wenlong Liu, Fufeng Liu, Huitu Zhang
  • ALKALINE PROTEASE MUTANT, AND GENE, ENGINEERED STRAIN, PREPARATION METHOD AND APPLICATION THEREOF
    Publication number: 20230016224
    Abstract: An alkaline protease mutant, and a gene, engineered strain, a preparation method and application thereof are provided. The method comprises the following steps of extracting genome DNA of Bacillus clausii, performing PCR amplification to obtain a wild-type alkaline protease gene sequence, mutating the wild-type alkaline protease gene obtained by the amplification through an error-prone PCR, performing high-throughput screening to obtain a plurality of highly active alkaline protease genes, performing DNA shuffling on the highly active alkaline protease genes, and performing screening to obtain eight alkaline protease mutant genes with higher activity.
    Type: Application
    Filed: July 31, 2022
    Publication date: January 19, 2023
    Applicants: Tianjin University of Science and Technology, Shandong Lonct Enzymes Co. LTD
    Inventors: Fuping LU, Yihan LIU, Yu LI, Xingji WANG, Kefen WANG, Wenlong LIU, Fufeng LIU, Huitu ZHANG
  • Glucoamylase mutant GA3 with improved specific activity and thermal stability, and gene and application thereof
    Patent number: 11512298
    Abstract: Glucoamylase mutant GA3 with improved specific activity and thermal stability, and its gene and application are provided, belonging to the field of gene engineering. The glucoamylase mutant GA3 of the present invention obtained by sequentially mutation at fixed points starting from wild glucoamylase processes both improved thermal stability and catalytic efficiency, and can be applied to the industries of feed, food, medicine and the like.
    Type: Grant
    Filed: February 6, 2021
    Date of Patent: November 29, 2022
    Assignee: Shandong Lonct Enzymes Co. LTD
    Inventors: Xingji Wang, Kefen Wang, Jie Zhang, Xinwei Tong, Wenlong Liu
  • Glucoamylase mutant GA3 with improved specific activity and thermal stability, and gene and application thereof
    Publication number: 20210163911
    Abstract: Glucoamylase mutant GA3 with improved specific activity and thermal stability, and its gene and application are provided, belonging to the field of gene engineering. The glucoamylase mutant GA3 of the present invention obtained by sequentially mutation at fixed points starting from wild glucoamylase prosesses both improved thermal stability and catalytic efficiency, and can be applied to the industries of feed, food, medicine and the like.
    Type: Application
    Filed: February 6, 2021
    Publication date: June 3, 2021
    Inventors: Xingji WANG, Kefen WANG, Jie ZHANG, Xinwei TONG, Wenlong LIU