Patents by Inventor Kei Endo
Kei Endo has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
-
Patent number: 11968339Abstract: A reading device includes a base on which a read target object is placed, a reader that reads the read target object placed on the base, a first attachment frame to which the reader is attached such that the reader is disposed above the base, an operation unit that is used for operating the reading device by touching, a second attachment frame to which the operation unit is attached such that the operation unit is disposed above the base, and a support frame that has a first surface and a second surface more bendable than the first surface in response to an external force. The first attachment frame is fixed to the first surface of the support frame. The second attachment frame is fixed to the second surface of the support frame.Type: GrantFiled: December 5, 2021Date of Patent: April 23, 2024Assignee: FUJIFILM Business Innovation Corp.Inventors: Taisuke Endo, Keigo Shinoto, Maho Satani, Kei Otagiri, Kei Kobayashi
-
Publication number: 20240033863Abstract: Provided is a flux for resin-cored solder that is used in resin-cored solder that is supplied into a through hole formed along a central axis of a soldering iron. The flux includes 60% by mass to 99.9% by mass of a rosin ester to a total mass of the flux, 0.1% by mass to 15% by mass of a covalent halogen compound to the total mass of the flux, and more than 0% by mass to 10% by mass of rosin amine, N,N-diethyloctylamine, or rosin amine and N,N-diethyloctylamine to the total mass of the flux.Type: ApplicationFiled: November 10, 2021Publication date: February 1, 2024Applicants: Senju Metal Industry Co., Ltd., DENSO CORPORATIONInventors: Yoko Kurasawa, Motohiro Onitsuka, Hisashi Tokutomi, Kei Endo, Kazuyuki Hamamoto
-
Publication number: 20230311226Abstract: A method for manufacturing a soldered product by soldering a solder object portion, including: a solder supplying step that causes a cylindrical soldering iron having a through hole to contact with the solder object portion to supply a thread solder piece to the solder object portion from the through hole; a heating step that heats the thread solder piece with the cylindrical soldering iron and causes to melt the thread solder piece at the solder object portion; and a curing step that cures a melting object of the thread solder piece to solder the solder object portion. The thread solder piece is composed of a core containing a flux and a coating member containing a solder alloy that covers the core. The flux has a rosin having an acid value which is substantially as a main component thereof.Type: ApplicationFiled: June 6, 2023Publication date: October 5, 2023Applicants: DENSO CORPORATION, SENJU METAL INDUSTRY CO., LTD.Inventors: Kei ENDO, Kazuyuki HAMAMOTO, Hisashi TOKUTOMI, Motohiro ONITSUKA, Yoko KURASAWA
-
Publication number: 20220395585Abstract: Engineered synthetic RNA-based genetic circuits are provided that are regulated exclusively at the post-transcriptional level.Type: ApplicationFiled: May 3, 2022Publication date: December 15, 2022Applicants: Massachusetts Institute of Technology, Kyoto UniversityInventors: Ron Weiss, Liliana Wroblewska, Velia Siciliano, Tasuku Kitada, Maria Hottelet Foley, Katie Bodner, Hirohide Saito, Kei Endo, Darrell J. Irvine, Tyler Wagner, Jacob Becraft
-
Patent number: 11351271Abstract: Engineered synthetic RNA-based genetic circuits are provided that are regulated exclusively at the post-transcriptional level.Type: GrantFiled: September 8, 2015Date of Patent: June 7, 2022Assignees: Massachusetts Institute of Technology, Kyoto UniversityInventors: Ron Weiss, Liliana Wroblewska, Velia Siciliano, Tasuku Kitada, Maria Hottelet Foley, Katie Bodner, Hirohide Saito, Kei Endo, Darrell J. Irvine, Tyler Wagner, Jacob Becraft
-
Publication number: 20200232049Abstract: A method is provided for distinguishing living cells in a living state with high accuracy. Provided is a method for distinguishing a desired cell type from a cell group comprising two or more types of cells, using the expression of miRNA as an indicator, wherein the method comprises the following steps: (1) a step of introducing mRNA comprising a marker gene operably linked to the target sequence of miRNA used as an indicator into a cell group; and (2) a step of distinguishing a cell type, using the translation level of the marker gene as an indicator.Type: ApplicationFiled: February 13, 2020Publication date: July 23, 2020Inventors: HIROHIDE SAITO, KEI ENDO
-
Patent number: 10604770Abstract: A method for extracting differentiated cells from a cell population comprising undifferentiated cells after induction of the differentiation of pluripotent stem cells. A method for extracting differentiated cells from a cell population, comprising the following steps: (1) a step of introducing, into a cell population, mRNA comprising a marker gene operably linked to the target sequence of miRNA specifically expressed in pluripotent stem cells; and (2) a step of extracting cells in which the marker gene has been translated.Type: GrantFiled: July 16, 2015Date of Patent: March 31, 2020Assignee: KYOTO UNIVERSITYInventors: Hirohide Saito, Kei Endo, Shota Katayama, Callum Parr
-
Patent number: 10590492Abstract: A method for distinguishing living cells in a living state with high accuracy. A method for distinguishing a desired cell type from a cell group comprising two or more types of cells, using the expression of miRNA as an indicator, wherein the method comprises the following steps: (1) a step of introducing mRNA comprising a marker gene operably linked to the target sequence of miRNA used as an indicator into a cell group; and (2) a step of distinguishing a cell type, using the translation level of the marker gene as an indicator.Type: GrantFiled: January 9, 2015Date of Patent: March 17, 2020Assignee: KYOTO UNIVERSITYInventors: Hirohide Saito, Kei Endo
-
Publication number: 20200056248Abstract: An object of the present invention is to provide a method for increasing the purity of a type of tissue cell such as an endothelial cell, a hepatocyte, or an insulin-producing cell. The present invention solves the problem by providing a method comprising a step of introducing, into a cell population, an mRNA comprising a nucleic acid sequence recognized by an miRNA specifically expressed in endothelial cells, hepatocytes, or insulin-producing cells.Type: ApplicationFiled: October 22, 2019Publication date: February 20, 2020Inventors: Yoshinori Yoshida, Hirohide Saito, Kenji Miki, Kei Endo, Seiya Takahashi
-
Patent number: 10538740Abstract: An object of the present invention is to provide a novel method for sorting cardiomyocytes. Another object of the present invention is to provide a method for producing high-purity cardiomyocytes and a kit used therefor. The present invention provides a method for sorting cardiomyocytes, comprising a step of introducing miRNA-responsive mRNA into a cell group, wherein the miRNA-responsive mRNA consists of a sequence comprising the following (i) and (ii): (i) a nucleic acid specifically recognized by miRNA specifically expressed in cardiomyocytes, and (ii) a nucleic acid corresponding to the coding region of a gene, wherein translation of (ii) the nucleic acid corresponding to the coding region of a gene into protein is regulated by the nucleic acid sequence in (i) above, thereby achieving the aforementioned objects.Type: GrantFiled: March 20, 2015Date of Patent: January 21, 2020Assignee: Kyoto UniversityInventors: Yoshinori Yoshida, Hirohide Saito, Kenji Miki, Kei Endo, Seiya Takahashi
-
Patent number: 10501811Abstract: An object of the present invention is to provide a method for increasing the purity of a type of tissue cell such as an endothelial cell, a hepatocyte, or an insulin-producing cell. The present invention solves the problem by providing a method comprising a step of introducing, into a cell population, an mRNA comprising a nucleic acid sequence recognized by an miRNA specifically expressed in endothelial cells, hepatocytes, or insulin-producing cells.Type: GrantFiled: April 22, 2016Date of Patent: December 10, 2019Assignee: KYOTO UNIVERSITYInventors: Yoshinori Yoshida, Hirohide Saito, Kenji Miki, Kei Endo, Seiya Takahashi
-
Publication number: 20190151474Abstract: Engineered synthetic RNA-based genetic circuits are provided that are regulated exclusively at the post-transcriptional level.Type: ApplicationFiled: September 8, 2015Publication date: May 23, 2019Applicants: Massachusetts Institute of Technology, Kyoto UniversityInventors: Ron Weiss, Liliana Wroblewska, Velia Siciliano, Tasuku Kitada, Maria Hottelet Foley, Katie Bodner, Hirohide Saito, Kei Endo, Darrell J. Irvine, Tyler Wagner, Jacob Becraft
-
Publication number: 20190144873Abstract: A translational control method using an RNA-protein interaction motif is provided. The method comprises a step of introducing an mRNA having: a 5?UTR regulation structure comprising: (1) a cap structure at the 5? terminus, (2) a spacer positioned on the 3? side of the cap structure, and (3) one or more RNA motifs positioned on the 3? side of the spacer, which comprises an RNA-protein interaction motif-derived nucleotide sequence or a variant thereof; and a nucleotide sequence encoding a target protein gene on the 3? side of the 5?UTR regulation structure, into a cell in the presence of a protein specifically binding to the RNA motifs, wherein a translational level is decreased as the number of bases of the spacer decreases, and the translational level is decreased as the number of the RNA motifs increases.Type: ApplicationFiled: January 25, 2019Publication date: May 16, 2019Applicant: Kyoto UniversityInventors: Hirohide Saito, Kei Endo, Tan Inoue
-
Patent number: 10227596Abstract: A translational control method using an RNA-protein interaction motif is provided. The method comprises a step of introducing an mRNA having: a 5?UTR regulation structure comprising: (1) a cap structure at the 5? terminus, (2) a spacer positioned on the 3? side of the cap structure, and (3) one or more RNA motifs positioned on the 3? side of the spacer, which comprises an RNA-protein interaction motif-derived nucleotide sequence or a variant thereof; and a nucleotide sequence encoding a target protein gene on the 3? side of the 5?UTR regulation structure, into a cell in the presence of a protein specifically binding to the RNA motifs, wherein a translational level is decreased as the number of bases of the spacer decreases, and the translational level is decreased as the number of the RNA motifs increases.Type: GrantFiled: August 24, 2016Date of Patent: March 12, 2019Assignee: Kyoto UniversityInventors: Hirohide Saito, Kei Endo, Tan Inoue
-
Publication number: 20180296702Abstract: Engineered synthetic RNA-based genetic circuits are provided that are regulated exclusively at the post-transcriptional level.Type: ApplicationFiled: September 8, 2015Publication date: October 18, 2018Applicants: Massachusetts Institute of Technology, Kyoto UniversityInventors: Ron Weiss, Liliana Wroblewska, Velia Siciliano, Tasuku Kitada, Maria Hottelet Foley, Katie Bodner, Hirohide Saito, Kei Endo, Darrell J. Irvine, Tyler Wagner, Jacob Becraft
-
Publication number: 20180100203Abstract: An object of the present invention is to provide a method for increasing the purity of a type of tissue cell such as an endothelial cell, a hepatocyte, or an insulin-producing cell. The present invention solves the problem by providing a method comprising a step of introducing, into a cell population, an mRNA comprising a nucleic acid sequence recognized by an miRNA specifically expressed in endothelial cells, hepatocytes, or insulin-producing cells.Type: ApplicationFiled: April 22, 2016Publication date: April 12, 2018Inventors: Yoshinori Yoshida, Hirohide Saito, Kenji Miki, Kei Endo, Seiya Takahashi
-
Publication number: 20170369846Abstract: An object of the present invention is to provide a novel method for sorting cardiomyocytes. Another object of the present invention is to provide a method for producing high-purity cardiomyocytes and a kit used therefor. The present invention provides a method for sorting cardiomyocytes, comprising a step of introducing miRNA-responsive mRNA into a cell group, wherein the miRNA-responsive mRNA consists of a sequence comprising the following (i) and (ii): (i) a nucleic acid specifically recognized by miRNA specifically expressed in cardiomyocytes, and (ii) a nucleic acid corresponding to the coding region of a gene, wherein translation of (ii) the nucleic acid corresponding to the coding region of a gene into protein is regulated by the nucleic acid sequence in (i) above, thereby achieving the aforementioned objects.Type: ApplicationFiled: March 20, 2015Publication date: December 28, 2017Inventors: Yoshinori Yoshida, Hirohide Saito, Kenji Miki, Kei Endo, Seiya Takahashi
-
Publication number: 20170342439Abstract: A method for extracting differentiated cells from a cell population comprising undifferentiated cells after induction of the differentiation of pluripotent stem cells. A method for extracting differentiated cells from a cell population, comprising the following steps: (1) a step of introducing, into a cell population, mRNA comprising a marker gene operably linked to the target sequence of miRNA specifically expressed in pluripotent stem cells; and (2) a step of extracting cells in which the marker gene has been translated.Type: ApplicationFiled: July 16, 2015Publication date: November 30, 2017Inventors: Hirohide Saito, Kei Endo, Shota Katayama, Callum Parr
-
Publication number: 20170051292Abstract: A translational control method using an RNA-protein interaction motif is provided. The method comprises a step of introducing an mRNA having: a 5?UTR regulation structure comprising: (1) a cap structure at the 5? terminus, (2) a spacer positioned on the 3? side of the cap structure, and (3) one or more RNA motifs positioned on the 3? side of the spacer, which comprises an RNA-protein interaction motif-derived nucleotide sequence or a variant thereof; and a nucleotide sequence encoding a target protein gene on the 3? side of the 5?UTR regulation structure, into a cell in the presence of a protein specifically binding to the RNA motifs, wherein a translational level is decreased as the number of bases of the spacer decreases, and the translational level is decreased as the number of the RNA motifs increases.Type: ApplicationFiled: August 24, 2016Publication date: February 23, 2017Applicant: KYOTO UNIVERSITYInventors: Hirohide Saito, Kei ENDO, Tan INOUE
-
Publication number: 20170016077Abstract: A method for distinguishing living cells in a living state with high accuracy. A method for distinguishing a desired cell type from a cell group comprising two or more types of cells, using the expression of miRNA as an indicator, wherein the method comprises the following steps: (1) a step of introducing mRNA comprising a marker gene operably linked to the target sequence of miRNA used as an indicator into a cell group; and (2) a step of distinguishing a cell type, using the translation level of the marker gene as an indicator.Type: ApplicationFiled: January 9, 2015Publication date: January 19, 2017Inventors: Hirohide Saito, Kei Endo