Abstract: A large current electron beam is stably emitted from an electron gun of a charged particle beam device. The electron gun of the charged particle beam device includes: a SE tip 202; a suppressor 303 disposed rearward of a distal end of the SE tip; a cup-shaped extraction electrode 204 including a bottom surface and a cylindrical portion and enclosing the SE tip and the suppressor; and an insulator 208 holding the suppressor and the extraction electrode. A shield electrode 301 of a conductive metal having a cylindrical portion 302 is provided between the suppressor and the cylindrical portion of the extraction electrode. A voltage lower than a voltage of the SE tip is applied to the shield electrode.

Abstract: A container for forming a cell aggregate is provided in which a group represented by a general formula of: (in the formula, each of R1, R2, and R3 is independently a an alkyl group with a carbon number of 1 or more and 6 or less and m is an integer of 2 or more and 6 or less.) and at least one of an amino group, a carboxyl group, and a hydroxyl group are present near a surface thereof.

Abstract: A method for forming a cell aggregate in the present invention is a method for forming a cell aggregate by using a culturing vessel and culturing an adhesive cell, wherein the culturing vessel is such that an amino group, a carboxyl group, and/or a hydroxyl group, and a group represented by a general formula of (in the formula, each of R1, R2, and R3 is independently an alkyl group with a carbon number greater than or equal to 1 and less than or equal to 6 and m is an integer greater than or equal to 2 and less than or equal to 6.) are present near a surface thereof and the adhesive cell is a mesenchymal stem cell, a hepatic cell, or a cancer cell.

Abstract: A surface modification method in the present invention is characterized by having a step of applying an application fluid including a compound represented by a general formula of: (in the formula, each of R1, R2, and R3 is independently an alkoxy group with a carbon number of 1 or more and 6 or less and R4 is an alkoxy group with a carbon number of 1 or more and 6 or less or an alkyl group with a carbon number of 1 or more and 6 or less.) and a hydrophilization agent having a functional group capable of producing a silanol group through hydrolysis thereof or a silanol group onto a material having a functional group capable of producing a silanol group through hydrolysis thereof or a silanol group on a surface thereof.

Abstract: A container for forming a cell aggregate is provided in which a group represented by a general formula of: (in the formula, each of R1, R2, and R3 is independently a an alkyl group with a carbon number of 1 or more and 6 or less and m is an integer of 2 or more and 6 or less.) and at least one of an amino group, a carboxyl group, and a hydroxyl group are present near a surface thereof.