Abstract: Disclosed herein are homology-independent targeted integration methods of integrating an exogenous DNA sequence into a genome of a non-dividing cell and compositions for such methods. Methods herein comprise contacting the non-dividing cell with a composition comprising a targeting construct comprising the exogenous DNA sequence and a targeting sequence, a complementary strand oligonucleotide homologous to the targeting sequence, and a nuclease, thereby altering the genome of the non-dividing cell.

Abstract: A method for manufacturing a lithium hydroxide powder includes a pulverizing step of pulverizing coarse particles of lithium hydroxide to obtain lithium hydroxide powder; and a carbonation-suppressing step of storing the lithium hydroxide powder in an airtight container in an atmosphere satisfying requirements (1) and (2). (1) A partial pressure of carbon dioxide gas is 100 Pa or less with respect to a total amount of gas present in the airtight container. (2) A relative humidity in the airtight container is 60% or less.

Abstract: A DC power supply device includes: a rectifier circuit; a reactor connected at one end to one output terminal of the rectifier circuit; a charging unit including a first and second switching element connected in series between another end of the reactor and another output terminal of the rectifier circuit, the charging unit configured to charge a first and second capacitor connected in series between output terminals to which a load is connected; and a control unit that controls the charging unit. The control unit sets, based on the timing at which the zero-crossing occurs, a dead time in which both the and second switching element are off, and when states of the first and second switching element at a time of occurrence of the zero-crossing match a predetermined set of states, reverses the states of the first switching element and the second switching element.

Abstract: A feed out container includes: a metal main body outer sleeve formed as a bottomed cylinder; a metal cylindrical inner member having a first portion provided within the interior of the main body outer sleeve and a second portion that constitutes an annular protrusion; an inner plate that holds a contained material; a lid body outer sleeve formed as a bottomed cylinder; a lid body inner sleeve provided within and in close contact with the interior of the lid body outer sleeve; and a feed out mechanism that moves the inner plate in an axial direction of the sleeve to feed out the contained material. At least one of a first O ring (31) that maintains an airtight seal between the lid body outer sleeve and the inner member and a second O ring fitted about the periphery of a portion of the inner member is provided.

Abstract: Disclosed herein are homology-independent targeted integration methods of integrating an exogenous DNA sequence into a genome of a non-dividing cell and compositions for such methods. Methods herein comprise contacting the non-dividing cell with a composition comprising a targeting construct comprising the exogenous DNA sequence and a targeting sequence, a complementary strand oligonucleotide homologous to the targeting sequence, and a nuclease, thereby altering the genome of the non-dividing cell.

Abstract: Disclosed herein are homology-independent targeted integration methods of integrating an exogenous DNA sequence into a genome of a non-dividing cell and compositions for such methods. Methods herein comprise contacting the non-dividing cell with a composition comprising a targeting construct comprising the exogenous DNA sequence and a targeting sequence, a complementary strand oligonucleotide homologous to the targeting sequence, and a nuclease, thereby altering the genome of the non-dividing cell.

Abstract: Provided herein are methods and compositions for editing a target genome in a cell comprising contacting the cell with (i) a single homology arm construct comprising a replacement sequence and a targeted endonuclease cleavage site; and (ii) a targeted endonuclease, wherein the replacement sequence comprises at least one nucleotide difference compared to the target genome and wherein the target genome comprises a sequence homologous to the targeted endonuclease cleavage site.

Abstract: A feed out container includes: a metal main body outer sleeve formed as a bottomed cylinder; a metal cylindrical inner member having a first portion provided within the interior of the main body outer sleeve and a second portion that constitutes an annular protrusion; an inner plate that holds a contained material; a lid body outer sleeve formed as a bottomed cylinder; a lid body inner sleeve provided within and in close contact with the interior of the lid body outer sleeve; and a feed out mechanism that moves the inner plate in an axial direction of the sleeve to feed out the contained material. At least one of a first O ring (31) that maintains an airtight seal between the lid body outer sleeve and the inner member and a second O ring fitted about the periphery of a portion of the inner member is provided.

Abstract: Systems and methods are disclosed for a system configured to access first content to determine that textual features common to queries received in a website are available in text within the first content. An identifier within the first content is determined where the identifier references second content that comprises details associated with the textual features. In response to a content query including the textual features received in the website, display content is generated. The display content includes a first area to display matching content indexed from prior user searches and also includes a second area to be modified. A portion of the details and the identifier is provided in the second area.

Abstract: A method for manufacturing a lithium hydroxide powder includes a pulverizing step of pulverizing coarse particles of lithium hydroxide to obtain lithium hydroxide powder; and a carbonation-suppressing step of storing the lithium hydroxide powder in an airtight container in an atmosphere satisfying requirements (1) and (2). (1) A partial pressure of carbon dioxide gas is 100 Pa or less with respect to a total amount of gas present in the airtight container. (2) A relative humidity in the airtight container is 60% or less.

Abstract: Disclosed herein are homology-independent targeted integration methods of integrating an exogenous DNA sequence into a genome of a non-dividing cell and compositions for such methods. Methods herein comprise contacting the non-dividing cell with a composition comprising a targeting construct comprising the exogenous DNA sequence and a targeting sequence, a complementary strand oligonucleotide homologous to the targeting sequence, and a nuclease, thereby altering the genome of the non-dividing cell.

Abstract: Embodiments described herein provide images representing a set of search results based on diversity between results of the search query. Images associated with a set of visually diverse items can be provided to provide a sample of items matching the search query across multiple types of categories. For example, search results can be grouped into types of categories and images from each of the types of categories can be grouped into subsets of visually related images (across one or more different visual attributes). A set of diverse representative images can be selected by taking at least one image from each of the groups of visually related images. The set of representative and diverse images can be displayed to provide an interesting, visually diverse, and aesthetically pleasing set of images to a user.

Abstract: A numerical control apparatus that controls a machine tool including a plurality of linear axes and rotation axes, and controls a relative tool posture with respect to work on a table includes a program reading unit that sequentially reads a tool posture with respect to the work from a machining program, a determining unit that determines whether the read tool posture is within a predetermined tool posture range, a tool-posture control unit that determines control content of the tool posture according to the determination by the determining unit, and an interpolation/acceleration and deceleration processing unit that controls the linear and rotation axes according to the control content of the tool posture. When the read tool posture is within the predetermined range, the tool-posture control unit determines rotation axis angles of the rotation axes such that a change of tool posture from the last tool posture is limited.

Abstract: Methods of preventing the transmission of a mitochondrial disease, disorder, or condition using mitochondria-targeted enzymes or mRNA encoding mitochondria-targeted enzymes. The methods as described herein can specifically eliminate mitochondrial DNA (mtDNA) mutations in the germline.

Abstract: The method for producing a fired body of the invention has the steps of: preparing a starting material mixture; molding the mixture to obtain a green molded body; a first heating step to make the molded body contain residual carbon-containing substances in a furnace by increasing the temperature of the furnace while introducing an oxygen containing gas; and a second heating step to obtain a fired body by further increasing the temperature of the furnace without introducing the oxygen containing gas; wherein the conditions for the first heating step are set so that when the molded body is allowed to stand after the first heating step in an oxygen-containing atmosphere at 900° C., the temperature at the center section of the molded body is at least 20° C. higher than the temperature of the atmosphere in the furnace.

Abstract: The present invention aims to provide a process for producing an aluminum titanate-based ceramics fired body wherein a cracking of a ceramics shaped body during a degreasing step is suppressed and also sufficient mechanical strength for handling is provided to the ceramics shaped body for such a case of the transfer from a degreasing step to a firing step. The present invention is a process for producing an aluminum titanate-based ceramics fired body comprising a shaping step of obtaining a ceramics shaped body by shaping a starting material mixture containing inorganic components including an aluminum source powder and a titanium source powder and organic components; a degreasing step for removing the organic components contained in the ceramics shaped body in atmosphere with an oxygen concentration of not higher than 0.1% and under the temperature condition that the highest temperature is not lower than 700° C. and not higher than 1100° C.

Abstract: A numerical control apparatus that controls a machine tool including a plurality of linear axes and rotation axes, and controls a relative tool posture with respect to work on a table includes a program reading unit that sequentially reads a tool posture with respect to the work from a machining program, a determining unit that determines whether the read tool posture is within a predetermined tool posture range, a tool-posture control unit that determines control content of the tool posture according to the determination by the determining unit, and an interpolation/acceleration and deceleration processing unit that controls the linear and rotation axes according to the control content of the tool posture. When the read tool posture is within the predetermined range, the tool-posture control unit determines rotation axis angles of the rotation axes such that a change of tool posture from the last tool posture is limited.

Abstract: The invention is to provide a process of producing the powder of aluminum titanate-based ceramics in which the formation of fine particulate component and coarse particulate component is inhibited, and having a very sharp grain size distribution, efficiently and at good yield. The invention is a process for producing a powder of aluminum titanate-based ceramics, comprising a step of keeping a precursor mixture containing a titanium source powder, an aluminum source powder and a silicon source powder at a temperature range of from 1100° C. to 1350° C. for 3 hours or more, followed by a step of heating the precursor mixture up to 1400° C.

Abstract: The tunnel kiln according to the present invention includes a tunnel kiln main body having an inner wall constituted by a furnace material, and having a debinding zone and a sintering zone wherein respectively debinding and sintering of a body to be fired containing an organic component are performed; a conveying unit for conveying the body to be fired from an inlet side to an outlet side of the tunnel kiln main body; and a lining provided so that at least an inner wall of the debinding zone of the inner wall of the tunnel kiln main body is covered therewith.

Abstract: A table-top game device includes a lobby control unit and a room control unit. The lobby control unit displays an image of a lobby, a virtual space where a plurality of users exchange information, and displays objects associated with the plurality of users at predetermined positions in the lobby. The room control unit displays an image of a room, a virtual space where the users in the lobby can enter, along with the image of the lobby so that the user entering the room can know the objects in the lobby.