Abstract: Provided is a solid-state imaging device that includes a plurality of photoelectric conversion units formed on a substrate to generate signal charges according to an amount of incident light, a microlens array including a microlens for a photoelectric conversion unit group including at least two or more adjacent photoelectric conversion units to guide incident light to the photoelectric conversion unit group, a scatterer on an optical path of the incident light collected by the microlens, and an inter-pixel light shielding portion including a groove between the photoelectric conversion unit of the photoelectric conversion unit group and the photoelectric conversion unit adjacent to the photoelectric conversion unit group and an insulating material filled in the groove. An opening side of an inner side surface of the groove on the scatterer side is a flat surface inclined so that a groove width becomes narrower toward a bottom of the groove.

Abstract: A reduction in image quality and a reduction in transfer efficiency are suppressed. An imaging apparatus according to an embodiment includes a plurality of pixels arrayed in a two-dimensional lattice pattern, in which each of the pixels includes a photoelectric conversion unit on a first surface side of a semiconductor substrate, a vertical gate electrode on the semiconductor substrate so as to be close to the photoelectric conversion unit from a second surface side opposite to the first surface, a gate insulating film between the vertical gate electrode and the semiconductor substrate, a transfer gate electrode connected to the vertical gate electrode on the second surface of the semiconductor substrate, and a first diffusion region on a second surface side of the semiconductor substrate. A diameter of the vertical gate electrode is greater on the photoelectric conversion unit side than on the transfer gate electrode side.

Abstract: Provided is a light detection device which allows influence on adjacent pixels to be reduced. The light detection device includes a first substrate portion, a second substrate portion, and a through via. The first substrate portion has a pixel configured to photoelectrically convert incident light. The second substrate portion has a readout circuit configured to output a pixel signal based on charge output from the pixel to a signal line. The through via configured to connect the first substrate portion and the second substrate portion. The pixel has a floating diffusion configured to temporarily retain charge generated by photoelectric conversion. The readout circuit has a first pixel transistor connected to the floating diffusion through the through via and a second pixel transistor connected to the first pixel transistor and the signal line.

Abstract: There is provided an imaging device including a semiconductor substrate and a plurality of imaging elements that are arrayed in a matrix in a first direction and a second direction on the semiconductor substrate and perform photoelectric conversion on incident light. Each of the plurality of imaging elements includes a plurality of pixels that are provided in a predetermined unit region of the semiconductor substrate to be adjacent to each other and contains impurities of a first conductivity type, a separation section that separates the plurality of pixels, two first element separation walls that are provided along two first side surfaces extending in the second direction of the predetermined unit region to pierce through at least a part of the semiconductor substrate, and a first diffusion region provided in the semiconductor substrate around the first element separation wall and the separation section and containing impurities of a second conductivity type.

Abstract: The present invention provides a complex containing a nucleic acid sequence-recognizing module and a proteolysis tag, wherein the module is linked to the proteolysis tag, the module specifically binds to a target nucleotide sequence in a double stranded DNA, and the tag consists of (i) a peptide containing 3 hydrophobic amino acid residues at the C-terminal, or (ii) a peptide containing 3 amino acid residues at the C-terminal wherein at least a part of the amino acid residues is substituted by serine.

Abstract: The present invention provides a method for selecting a promoter that functions in an organelle, the method comprising: (1) the step of preparing sequence information obtained by RNA sequencing analysis; (2) the step of mapping the sequence information prepared in the step (1) onto a sequence of organellar DNA; (3) the step of calculating the amount of change in RNA expression in each region based on the mapping information obtained in the step (2); (4) the step of selecting regions in which the amount of change obtained in the step (3) is within a range of preset reference values; and (5) the step of identifying a region, among the regions selected, as a promoter functioning in an organelle.

Abstract: The present invention provides a complex containing a nucleic acid sequence-recognizing module and a proteolysis tag, wherein the module is linked to the proteolysis tag, the module specifically binds to a target nucleotide sequence in a double stranded DNA, and the tag consists of (i) a peptide containing 3 hydrophobic amino acid residues at the C-terminal, or (ii) a peptide containing 3 amino acid residues at the C-terminal wherein at least a part of the amino acid residues is substituted by serine

Abstract: The invention provides a method for producing a plant cell modified at a targeted site of a double-stranded DNA, including (i) a step of providing a plant cell comprising the double-stranded DNA of interest, (ii) a step of providing a complex in which a nucleic acid sequence-recognizing module that specifically binds to a target nucleotide sequence in the double-stranded DNA and a DNA glycosylase with sufficiently low reactivity with the double-stranded DNA are bound, (iii) a step of placing the complex in a condition under which the plant cell is transfected, (iv) a step of placing the transfected plant cell in a condition that induces modification of the targeted site, without cleaving at least one strand of the double-stranded DNA in the targeted site, and (v) a step of selecting a cell into which the complex has been introduced and/or a cell into which the modification has been introduced.

Abstract: A solid-state imaging element (1) according to the present disclosure includes a plurality of light-receiving pixels (11) arranged in a matrix inside a semiconductor layer (20). The light-receiving pixel (11) includes a pair of photoelectric conversion units, a first separation region (24), a second separation region (25), and a third separation region (26). The pair of photoelectric conversion units are disposed adjacent to each other. The first separation region (24) is disposed so as to surround the pair of photoelectric conversion units and is disposed so as to penetrate the semiconductor layer (20). The second separation region (25) is disposed between the pair of photoelectric conversion units and is disposed so as to penetrate the semiconductor layer (20). The third separation region (26) is disposed in a region surrounded by the first separation region (24) and is disposed from a light incident surface (20a) of the semiconductor layer (20) to a middle of the semiconductor layer (20).

Abstract: The invention provides a method for producing a plant cell modified at a targeted site of a double-stranded DNA, including (i) a step of providing a plant cell comprising the double-stranded DNA of interest, (ii) a step of providing a complex in which a nucleic acid sequence-recognizing module that specifically binds to a target nucleotide sequence in the double-stranded DNA and a DNA glycosylase with sufficiently low reactivity with the double-stranded DNA are bound, (iii) a step of placing the complex in a condition under which the plant cell is transfected, (iv) a step of placing the transfected plant cell in a condition that induces modification of the targeted site, without cleaving at least one strand of the double-stranded DNA in the targeted site, and (v) a step of selecting a cell into which the complex has been introduced and/or a cell into which the modification has been introduced.

Abstract: Provided is a method for altering a targeted site of a DNA in a cell, including a step of stimulating the cell with a factor inducing a DNA modifying enzyme endogenous to the cell, and bringing a complex of a nucleic acid sequence-recognizing module specifically binding to a target nucleotide sequence in a given DNA and a DNA modifying enzyme-binding module bonded to each other into contact with the DNA to convert one or more nucleotides in the targeted site to other one or more nucleotides or delete one or more nucleotides, or insert one or more nucleotides into the targeted site.

Abstract: The invention provides a site-specific modification method of a DNA molecule that enables gene function manipulation. In particular, the invention provides a method for manipulating gene function by a site-specific action on a target DNA molecule, said method comprising a step for preparing a complex containing an Argonaute protein, a guide RNA and an additional sequence and a step for contacting the target DNA molecule with the complex, wherein the guide RNA is designed to guide the complex to a region containing the desired site in the target DNA molecule.

Abstract: The present disclosure relates to a solid-state imaging device, an imaging device, and an electronic apparatus that are capable of suppressing generation of flare and also suppressing coloring caused by the flare with a simple configuration. A high refractive index layer is formed between a solid-state imaging element and a transparent protective substrate (glass substrate). When reflected light caused by diffracted light generated from an on-chip lens is reflected at an interface with the high refractive index layer, the reflected light is entirely reflected at a surface layer that is a transparent protective substrate and then the reflected light is sufficiently attenuated before being incident again. Consequently, flare and coloring caused by the flare are suppressed. The present disclosure is adaptable to an imaging device.

Abstract: Provided is a method for altering a targeted site of a DNA in a cell, including a step of stimulating the cell with a factor inducing a DNA modifying enzyme endogenous to the cell, and bringing a complex of a nucleic acid sequence-recognizing module specifically binding to a target nucleotide sequence in a given DNA and a DNA modifying enzyme-binding module bonded to each other into contact with the DNA to convert one or more nucleotides in the targeted site to other one or more nucleotides or delete one or more nucleotides, or insert one or more nucleotides into the targeted site.

Abstract: The invention provides a miniaturized cytidine deaminase-containing complex for modifying DNA formed by combining a nucleic acid sequence recognition module and cytidine deaminase, wherein the nucleic acid sequence recognition module specifically binds to a target nucleotide sequence of double-stranded DNA, the cytidine deaminase is composed of an amino acid sequence composed of a region of amino acid residues at positions 30-150 of SEQ ID NO: 1, an ortholog thereof, an amino acid sequence having mutations of one or several amino acids therein, or an amino acid sequence having at least 90% similarity thereto, and the targeted site of the double-stranded DNA is modified.

Abstract: The present disclosure relates to a solid-state imaging device, an imaging device, and an electronic apparatus that are capable of suppressing generation of flare and also suppressing coloring caused by the flare with a simple configuration. A high refractive index layer is formed between a solid-state imaging element and a transparent protective substrate (glass substrate). When reflected light caused by diffracted light generated from an on-chip lens is reflected at an interface with the high refractive index layer, the reflected light is entirely reflected at a surface layer that is a transparent protective substrate and then the reflected light is sufficiently attenuated before being incident again. Consequently, flare and coloring caused by the flare are suppressed. The present disclosure is adaptable to an imaging device.

Abstract: The invention provides a method of modifying a targeted site of a double stranded DNA, including a step of contacting a complex wherein a nucleic acid sequence-recognizing module that specifically binds to a target nucleotide sequence in a selected double stranded DNA and a nucleic acid base converting enzyme are linked, with the double stranded DNA, to convert one or more nucleotides in the targeted site to other one or more nucleotides or delete one or more nucleotides, or insert one or more nucleotides into the targeted site, without cleaving at least one strand of the double stranded DNA in the targeted site.

Abstract: The present invention provides an organelle transformant screening method. More specifically, the present invention provides an organelle transformant screening method utilizing inhibition of a lipid synthetic pathway.

Abstract: An imaging device includes a plurality of imaging elements, wherein each of the plurality of imaging elements includes: a plurality of pixels containing impurities of a first conductivity type; an element separation wall surrounding the plurality of pixels and provided so as to penetrate a semiconductor substrate; an on-chip lens provided above a light receiving surface of the semiconductor substrate so as to be shared by the plurality of pixels; and a first separation portion provided in a region surrounded by the element separation wall and separating the plurality of pixels, the first separation portion is provided so as to extend in a thickness direction of the semiconductor substrate, and a first diffusion region containing impurities of a second conductivity type opposite to the first conductivity type is provided in a region positioned around the first separation portion and extending in the thickness direction of the semiconductor substrate.

Abstract: Provided is a solid-state imaging device capable of suppressing color mixing between different colors while reducing the sensitivity difference between same colors. The solid-state imaging device includes: a plurality of photoelectric conversion units formed on a substrate to generate signal charges according to an amount of incident light; a microlens array including a microlens formed for a photoelectric conversion unit group including at least two or more adjacent photoelectric conversion units 21 to guide incident light to the photoelectric conversion unit group; a scatterer disposed on an optical path of the incident light collected by the microlens; and an inter-pixel light shielding portion including a groove formed between the photoelectric conversion unit of the photoelectric conversion unit group and the photoelectric conversion unit adjacent to the photoelectric conversion unit group and an insulating material filled in the groove.