Patents by Inventor Keiji Tsusaki
Keiji Tsusaki has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
-
Patent number: 10076130Abstract: The present invention has objects to provide a glucan useful as water-soluble dietary fiber, its preparation and uses. The present invention solves the above objects by providing a branched ?-glucan, which is constructed by glucose molecules and characterized by methylation analysis as follows: (1) Ratio of 2,3,6-trimethyl-1,4,5-triacetyl-glucitol to 2,3,4-trimethyl-1,5,6-triacetyl-glucitol is in the range of 1:0.6 to 1:4; (2) Total content of 2,3,6-trimethyl-1,4,5-triacetyl-glucitol and 2,3,4-trimethyl-1,5,6-triacetyl-glucitol is 60% or higher in the partially methylated glucitol acetates; (3) Content of 2,4,6-trimethyl-1,3,5-triacetyl-glucitol is 0.5% or higher but less than 10% in the partially methylated glucitol acetates; and (4) Content of 2,4-dimethyl-1,3,5,6-tetraacetyl-glucitol is 0.5% or higher in the partially methylated glucitol acetates; a novel ?-glucosyltransferase which forms the branched ?-glucan, processes for producing them, and their uses.Type: GrantFiled: November 3, 2016Date of Patent: September 18, 2018Assignee: HAYASHIBARA CO., LTD.Inventors: Hikaru Watanabe, Takuo Yamamoto, Tomoyuki Nishimoto, Keiji Tsusaki, Kazuyuki Oku, Hiroto Chaen, Shigeharu Fukuda
-
Publication number: 20170049142Abstract: The present invention has objects to provide a glucan useful as water-soluble dietary fiber, its preparation and uses. The present invention solves the above objects by providing a branched ?-glucan, which is constructed by glucose molecules and characterized by methylation analysis as follows: (1) Ratio of 2,3,6-trimethyl-1,4,5-triacetyl-glucitol to 2,3,4-trimethyl-1,5,6-triacetyl-glucitol is in the range of 1:0.6 to 1:4; (2) Total content of 2,3,6-trimethyl-1,4,5-triacetyl-glucitol and 2,3,4-trimethyl-1,5,6-triacetyl-glucitol is 60% or higher in the partially methylated glucitol acetates; (3) Content of 2,4,6-trimethyl-1,3,5-triacetyl-glucitol is 0.5% or higher but less than 10% in the partially methylated glucitol acetates; and (4) Content of 2,4-dimethyl-1,3,5,6-tetraacetyl-glucitol is 0.5% or higher in the partially methylated glucitol acetates; a novel ?-glucosyltransferase which forms the branched ?-glucan, processes for producing them, and their uses.Type: ApplicationFiled: November 3, 2016Publication date: February 23, 2017Inventors: Hikaru Watanabe, Takuo Yamamoto, Tomoyuki Nishimoto, Keiji Tsusaki, Kazuyuki Oku, Hiroto Chaen, Shigeharu Fukuda
-
Patent number: 9528134Abstract: The present invention has objects to provide a glucan useful as water-soluble dietary fiber, its preparation and uses. The present invention solves the above objects by providing a branched ?-glucan, which is constructed by glucose molecules and characterized by methylation analysis as follows: (1) Ratio of 2,3,6-trimethyl-1,4,5-triacetyl-glucitol to 2,3,4-trimethyl-1,5,6-triacetyl-glucitol is in the range of 1:0.6 to 1:4; (2) Total content of 2,3,6-trimethyl-1,4,5-triacetyl-glucitol and 2,3,4-trimethyl-1,5,6-triacetyl-glucitol is 60% or higher in the partially methylated glucitol acetates; (3) Content of 2,4,6-trimethyl-1,3,5-triacetyl-glucitol is 0.5% or higher but less than 10% in the partially methylated glucitol acetates; and (4) Content of 2,4-dimethyl-1,3,5,6-tetraacetyl-glucitol is 0.5% or higher in the partially methylated glucitol acetates; a novel ?-glucosyltransferase which forms the branched ?-glucan, processes for producing them, and their uses.Type: GrantFiled: June 9, 2015Date of Patent: December 27, 2016Assignee: HAYASHIBARA CO., LTD.Inventors: Hikaru Watanabe, Takuo Yamamoto, Tomoyuki Nishimoto, Keiji Tsusaki, Kazuyuki Oku, Hiroto Chaen, Shigeharu Fukuda
-
Patent number: 8759030Abstract: The object of the present invention is to provide a method and a process for producing 2-O-?-glucopyranosyl-L-ascorbic acid where 5-O-?-glucopyranosyl-L-ascorbic acid and 6-O-?-glucopyranosyl-L-ascorbic acid are not formed or formed in such a small amount that the formation of these can nor be detected. The present invention solves the above object by providing a process for producing 2-O-?-glucopyranosyl-L-ascorbic acid comprising the steps of allowing ?-isomaltosyl glucosaccharide-forming enzyme together with or without cyclomaltodextrin glucanotransferase (EC 2.4.1.19) to act on a solution comprising L-ascorbic acid and, an ?-glucosyl saccharide to form 2-O-?-glucopyranosyl-L-ascorbic acid and collecting the formed 2-O-?-glucopyranosyl-L-ascorbic acid.Type: GrantFiled: July 7, 2003Date of Patent: June 24, 2014Assignee: Hayashibara Co., Ltd.Inventors: Kazuhisa Mukai, Keiji Tsusaki, Michio Kubota, Shigeharu Fukuda, Toshio Miyake
-
Patent number: 8530222Abstract: Problem: To provide a microorganism with an ability to produce deoxy polyol dehydrogenase. Means for Resolution: A microorganism belonging to genus Enterobacter with an ability to produce a dehydrogenase for deoxy polyol of the same structure at the positions C2 and C3 as that of ribitol or L-iditol. The bacterial cell IK7 of the genus Enterobacter (accession No. NITE P-271). A method for producing deoxy ketose comprising allowing a culture containing the deoxy polyol dehydrogenase obtained by the culturing of the microorganism of the invention or allowing the deoxy polyol dehydrogenase to react with a solution containing deoxy polyol of the same structure at the positions C2 and C3 as that of ribitol or L-iditol to oxidize deoxy polyol to produce the corresponding deoxy ketose and then collecting the deoxy ketose. The deoxy polyol is 1-deoxy-D-allitol, while the corresponding deoxy ketose is 1-deoxy-L-psicose.Type: GrantFiled: March 27, 2007Date of Patent: September 10, 2013Assignees: National University Corporation Kagawa University, Rare Sugar Production Technical Research Laboratories, LLC., Matsutani Chemical Industry Co., Ltd.Inventors: Ken Izumori, Kenji Morimoto, Goro Takata, Masaaki Tokuda, Yoshio Tsujisaka, Kei Takeshita, Keiji Tsusaki, Kazuhiro Okuma
-
Patent number: 8420606Abstract: A method of utilizing the physiological activity of a rare saccharide, wherein physiological-activity sensitive cells are treated with the rare saccharide to modify the function of the cells. A composition containing, as an active ingredient, a rare saccharide which is introduced into physiological-activity sensitive cells and has an effect of modifying the function of the cells. The cells are human cells. The composition is a functional food, a drug, or a cosmetic. The rare saccharide is a rare saccharide belonging to aldose and/or ketose. The aldose is D-allose, and the cells are selected from the group consisting of cancer-cell proliferation inhibitory activity sensitive cells and active-oxygen production inhibitory activity sensitive cells. The ketose is D-psicose, and the cells are selected from the group consisting of chemokine secretion inhibitory activity sensitive cells, microglia migration inhibitory activity sensitive cells, and hypoglycemic activity sensitive cells.Type: GrantFiled: January 14, 2011Date of Patent: April 16, 2013Assignees: Fushimi Pharmaceutical Co., Ltd., Teikoku Seiyaku Co., Ltd., National University Corporation Kagawa University, Kabushiki Kaisha Hayashibara Seibutsu Kagaku KenkyujoInventors: Ken Izumori, Masaaki Tokuda, Toshifumi Itano, Osamu Miyamoto, Toshihiko Ishida, Koji Murao, Taizo Tasaka, Gan Muneuchi, Fuminori Yamaguchi, Terukazu Tanaka, Masaaki Ueki, Kazuyuki Hirooka, Tomohiko Taminato, Mohammad A. Hossain, Keiji Tsusaki, Takeo Takahashi, Mitsuhiro Nagata, Yutaka Ishida
-
Patent number: 8389248Abstract: Providing 1- or 6-deoxy products corresponding to all of aldohexoses, ketohexoses and sugar alcohols, as based on Deoxy-Izumoring, as well as a method for systematically producing those products. A method for producing deoxyketohexose and a derivative thereof using a deoxyketohexose isomerase derived from Pseudomonas cichorii ST-24 (FERM BP-2736), comprising epimerizing 1-deoxy D-ketohexose or 6-deoxy D-ketohexose or 1-deoxy L-ketohexose or 6-deoxy L-ketohexose at position 3 to produce the individually corresponding 1-deoxy D-ketohexose or 6-deoxy D-ketohexose or 1-deoxy L-ketohexose or 6-deoxy L-ketohexose as an intended product.Type: GrantFiled: November 20, 2007Date of Patent: March 5, 2013Assignees: National University Corporation Kagawa University, Rare Sugar Production Technical Research Laboratories, LLC., Hayashibara Co., Ltd., Matsutani Chemical Industry Co., Ltd.Inventors: Ken Izumori, Masaaki Tokuda, George Fleet, Yoshio Tsujisaka, Kei Takeshita, Keiji Tsusaki, Kazuhiro Okuma
-
Patent number: 8324375Abstract: The present invention has objects to provide a glucan useful as water-soluble dietary fiber, its preparation and uses. The present invention solves the above objects by providing a branched ?-glucan, which is constructed by glucose molecules and characterized by methylation analysis as follows: (1) Ratio of 2,3,6-trimethyl-1,4,5-triacetyl-glucitol to 2,3,4-trimethyl-1,5,6-triacetyl-glucitol is in the range of 1:0.6 to 1:4; (2) Total content of 2,3,6-trimethyl-1,4,5-triacetyl-glucitol and 2,3,4-trimethyl-1,5,6-triacetyl-glucitol is 60% or higher in the partially methylated glucitol acetates; (3) Content of 2,4,6-trimethyl-1,3,5-triacetyl-glucitol is 0.5% or higher but less than 10% in the partially methylated glucitol acetates; and (4) Content of 2,4-dimethyl-1,3,5,6-tetraacetyl-glucitol is 0.5% or higher in the partially methylated glucitol acetates; a novel ?-glucosyltransferase which forms the branched ?-glucan, processes for producing them, and their uses.Type: GrantFiled: April 23, 2008Date of Patent: December 4, 2012Assignee: Hayashibara Co., Ltd.Inventors: Hikaru Watanabe, Takuo Yamamoto, Tomoyuki Nishimoto, Keiji Tsusaki, Kazuyuki Oku, Hiroto Chaen, Shigeharu Fukuda
-
Patent number: 8227232Abstract: Object: To provide a thermostable L-ribose isomerase. Means for Resolution: The thermostable L-ribose isomerase with MW. 32,000 (by SDS-PAGE), optimal temperature of 45° C., optimal pH of pH 9.0 (glycine-NaOH buffer), and stable physicochemical properties such as temperature stability up to 45° C. during thermal treatment at pH 9.0 for 10 minutes, and with an action to isomerize L-ribose to generate L-ribulose or of inversely to isomerize L-ribulose to generate L-ribose.Type: GrantFiled: March 26, 2007Date of Patent: July 24, 2012Assignees: National University Corporation Kagawa Univeristy, Rare Sugar Production Technical Research Laboratories, LLC., Matsutani Chemical Industry Co., Ltd.Inventors: Ken Izumori, Kenji Morimoto, Goro Takata, Masaaki Tokuda, Yoshio Tsujisaka, Kei Takeshita, Keiji Tsusaki, Kazuhiro Okuma
-
Publication number: 20110112043Abstract: A method of utilizing the physiological activity of a rare saccharide, wherein physiological-activity sensitive cells are treated with the rare saccharide to modify the function of the cells. A composition containing, as an active ingredient, a rare saccharide which is introduced into physiological-activity sensitive cells and has an effect of modifying the function of the cells. The cells are human cells. The composition is a functional food, a drug, or a cosmetic. The rare saccharide is a rare saccharide belonging to aldose and/or ketose. The aldose is D-allose, and the cells are selected from the group consisting of cancer-cell proliferation inhibitory activity sensitive cells and active-oxygen production inhibitory activity sensitive cells. The ketose is D-psicose, and the cells are selected from the group consisting of chemokine secretion inhibitory activity sensitive cells, microglia migration inhibitory activity sensitive cells, and hypoglycemic activity sensitive cells.Type: ApplicationFiled: January 14, 2011Publication date: May 12, 2011Applicants: NATIONAL UNIVERSITY CORPORATION KAGAWA UNIVERSITY, MATSUTANI CHEMICAL INDUSTRY CO., LTD.Inventors: Ken Izumori, Masaaki Tokuda, Toshifumi Itano, Osamu Miyamoto, Toshihiko Ishida, Koji Murao, Taizo Tasaka, Gan Muneuchi, Fuminori Yamaguchi, Terukazu Tanaka, Masaaki Ueki, Kazuyuki Hirooka, Tomohiko Taminato, Mohammad Akram Hossain, Keiji Tsusaki, Takeo Takahashi, Mitsuhiro Nagata, Yutaka Ishida
-
Patent number: 7906487Abstract: A method of utilizing the physiological activity of a rare saccharide, wherein physiological-activity sensitive cells are treated with the rare saccharide to modify the function of the cells. A composition containing, as an active ingredient, a rare saccharide which is introduced into physiological-activity sensitive cells and has an effect of modifying the function of the cells. The cells are human cells. The composition is a functional food, a drug, or a cosmetic. The rare saccharide is a rare saccharide belonging to aldose and/or ketose. The aldose is D-allose, and the cells are selected from the group consisting of cancer-cell proliferation inhibitory activity sensitive cells and active-oxygen production inhibitory activity sensitive cells. The ketose is D-psicose, and the cells are selected from the group consisting of chemokine secretion inhibitory activity sensitive cells, microglia migration inhibitory activity sensitive cells, and hypoglycemic activity sensitive cells.Type: GrantFiled: May 22, 2003Date of Patent: March 15, 2011Assignees: Fushimi Pharmaceutical Co., Ltd., Teikoku Seiyaku Co., Ltd., National University Corporation Kagawa University, Kabushiki Kaisha Hayashibara Seibutsu Kagaku KenkyujoInventors: Ken Izumori, Masaaki Tokuda, Toshifumi Itano, Osamu Miyamoto, Toshihiko Ishida, Koji Murao, Taizo Tasaka, Gan Muneuchi, Fuminori Yamaguchi, Terukazu Tanaka, Masaaki Ueki, Kazuyuki Hirooka, Tomohiko Taminato, Mohammad Akram Hossain, Keiji Tsusaki, Takeo Takahashi, Mitsuhiro Nagata, Yutaka Ishida
-
Patent number: 7811797Abstract: The object of the present invention is to provide an ?-isomaltosylglucosaccharide-forming enzyme, process of the same, cyclotetrasaccharide, and saccharide composition comprising the saccharide which are obtainable by using the enzyme; and is solved by establishing an ?-isomaltosylglucosaccharide-forming enzyme which forms a saccharide, having a glucose polymerization degree of at least three and having both the ?-1,6 glucosidic linkage as a linkage at the non-reducing end and the ?-1,4 glucosidic linkage other than the linkage at the non-reducing end, by catalyzing the ?-glucosyl-transfer from a saccharide having a glucose polymerization degree of at least two and having the ?-1,4 glucosidic linkage as a linkage at the non-reducing end without substantially increasing the reducing power; ?-isomaltosyl-transferring method using the enzyme; method for forming ?-isomaltosylglucosaccharide; process for producing a cyclotetrasaccharide having the structure of cyclo{66)-?-D-glucopyranosyl-(163)-?-D-glucopyranosyl-Type: GrantFiled: May 9, 2007Date of Patent: October 12, 2010Assignee: Kabushiki Kaisha Hayashibara Seibutsu Kagaku KenkyujoInventors: Michio Kubota, Keiji Tsusaki, Takanobu Higashiyama, Shigeharu Fukuda, Toshio Miyake
-
Publication number: 20100167350Abstract: Problem: To provide a microorganism with an ability to produce deoxy polyol dehydrogenase. Means for Resolution: A microorganism belonging to genus Enterobacter with an ability to produce a dehydrogenase for deoxy polyol of the same structure at the positions C2 and C3 as that of ribitol or L-iditol. The bacterial cell IK7 of the genus Enterobacter (accession No. NITE P-271). A method for producing deoxy ketose comprising allowing a culture containing the deoxy polyol dehydrogenase obtained by the culturing of the microorganism of the invention or allowing the deoxy polyol dehydrogenase to react with a solution containing deoxy polyol of the same structure at the positions C2 and C3 as that of ribitol or L-iditol to oxidize deoxy polyol to produce the corresponding deoxy ketose and then collecting the deoxy ketose. The deoxy polyol is 1-deoxy-D-allitol, while the corresponding deoxy ketose is 1-deoxy-L-psicose.Type: ApplicationFiled: March 27, 2007Publication date: July 1, 2010Applicants: NAT'L UNIVERSITY CORPORATION KAGAWA UNIVERSITY, RARE SUGAR PRODUCTION TECHNICAL RESEARCH LABS., MATSUTANI CHEMICAL INDUSTRY CO., LTD.Inventors: Ken Izumori, Kenji Morimoto, Goro Takata, Masaaki Tokuda, Yoshio Tsujisaka, Kei Takeshita, Keiji Tsusaki, Kazuhiro Okuma
-
Patent number: 7718404Abstract: The object of the present invention is to provide an ?-isomaltosylglucosaccharide-forming enzyme, process of the same, cyclotetrasaccharide, and saccharide composition comprising the saccharide which are obtainable by using the enzyme; and is solved by establishing an ?-isomaltosylglucosaccharide-forming enzyme which forms a saccharide, having a glucose polymerization degree of at least three and having both the ?-1,6 glucosidic linkage as a linkage at the non-reducing end and the ?-1,4 glucosidic linkage other than the linkage at the non-reducing end, by catalyzing the ?-glucosyl-transfer from a saccharide having a glucose polymerization degree of at least two and having the ?-1,4 glucosidic linkage as a linkage at the non-reducing end without substantially increasing the reducing power; ?-isomaltosyl-transferring method using the enzyme; method for forming ?-isomaltosylglucosaccharide; process for producing a cyclotetrasaccharide having the structure of cyclo{66)-?-D-glucopyranosyl-(163)-?-D-glucopyranosyl-Type: GrantFiled: May 9, 2007Date of Patent: May 18, 2010Assignee: Kabushiki Kaisha Hayashibara Seibutsu Kagaku KenkyujoInventors: Michio Kubota, Keiji Tsusaki, Takanobu Higashiyama, Shigeharu Fukuda, Toshio Miyake
-
Publication number: 20100120710Abstract: The present invention has objects to provide a glucan useful as water-soluble dietary fiber, its preparation and uses. The present invention solves the above objects by providing a branched ?-glucan, which is constructed by glucose molecules and characterized by methylation analysis as follows: (1) Ratio of 2,3,6-trimethyl-1,4,5-triacetyl-glucitol to 2,3,4-trimethyl-1,5,6-triacetyl-glucitol is in the range of 1:0.6 to 1:4; (2) Total content of 2,3,6-trimethyl-1,4,5-triacetyl-glucitol and 2,3,4-trimethyl-1,5,6-triacetyl-glucitol is 60% or higher in the partially methylated glucitol acetates; (3) Content of 2,4,6-trimethyl-1,3,5-triacetyl-glucitol is 0.5% or higher but less than 10% in the partially methylated glucitol acetates; and (4) Content of 2,4-dimethyl-1,3,5,6-tetraacetyl-glucitol is 0.5% or higher in the partially methylated glucitol acetates; a novel ?-glucosyltransferase which forms the branched ?-glucan, processes for producing them, and their uses.Type: ApplicationFiled: April 23, 2008Publication date: May 13, 2010Inventors: Hikaru Watanabe, Takuo Yamamoto, Tomoyuki Nishimoto, Keiji Tsusaki, Kazuyuki Oku, Hiroto Chaen, Shigeharu Fukuda
-
Publication number: 20100105885Abstract: Providing 1- or 6-deoxy products corresponding to all of aldohexoses, ketohexoses and sugar alcohols, as based on Deoxy-Izumoring, as well as a method for systematically producing those products. A method for producing deoxyketohexose and a derivative thereof using a deoxyketohexose isomerase derived from Pseudomonas cichorii ST-24 (FERM BP-2736), comprising epimerizing 1-deoxy D-ketohexose or 6-deoxy D-ketohexose or 1-deoxy L-ketohexose or 6-deoxy L-ketohexose at position 3 to produce the individually corresponding 1-deoxy D-ketohexose or 6-deoxy D-ketohexose or 1-deoxy L-ketohexose or 6-deoxy L-ketohexose as an intended product.Type: ApplicationFiled: November 20, 2007Publication date: April 29, 2010Applicants: NAT'L UNIVERSITY CORPORATION KAGAWA UNIVERSITY, RARE SUGAR PRODUCTION TECHNICAL RESEARCH LABS., MATSUTANI CHEMICAL INDUSTRY CO., LTD., KABUSHIKI KAISHA HAYASHIBARA SEIBUTSU KAGAKU KENKYUJOInventors: Ken Izumori, Masaaki Tokuda, George Fleet, Yoshio Tsujisaka, Kei Takeshita, Keiji Tsusaki, Kazuhiro Okuma
-
Publication number: 20090325231Abstract: Object: To provide a thermostable L-ribose isomerase. Means for Resolution: The thermostable L-ribose isomerase with MW. 32,000 (by SDS-PAGE), optimal temperature of 45° C., optimal pH of pH 9.0 (glycine-NaOH buffer), and stable physicochemical properties such as temperature stability up to 45° C. during thermal treatment at pH 9.0 for 10 minutes, and with an action to isomerize L-ribose to generate L-ribulose or of inversely to isomerize L-ribulose to generate L-ribose.Type: ApplicationFiled: March 26, 2007Publication date: December 31, 2009Applicants: NAT'L UNIVERSITY CORPORATION KAGAWA UNIVERSITY, RARE SUGAR PRODUCTION TECHNICAL RESEARCH LABS., MATSUTANI CHEMICAL INDUSTRY CO., LTD., KABUSHIKI KAISHA HAYASHIBARA SEIBUTSU KAGAKU KENKUJOInventors: Ken Izumori, Kenji Morimoto, Goro Takata, Masaaki Tokuda, Yoshio Tsujisaka, Kei Takeshita, Keiji Tsusaki, Kazuhiro Okuma
-
Publication number: 20080032350Abstract: The object of the present invention is to provide an ?-isomaltosylglucosaccharide-forming enzyme, process of the same, cyclotetrasaccharide, and saccharide composition comprising the saccharide which are obtainable by using the enzyme; and is solved by establishing an ?-isomaltosylglucosaccharide-forming enzyme which forms a saccharide, having a glucose polymerization degree of at least three and having both the ?-1,6 glucosidic linkage as a linkage at the non-reducing end and the ?-1,4 glucosidic linkage other than the linkage at the non-reducing end, by catalyzing the ?-glucosyl-transfer from a saccharide having a glucose polymerization degree of at least two and having the ?-1,4 glucosidic linkage as a linkage at the non-reducing end without substantially increasing the reducing power; ?-isomaltosyl-transferring method using the enzyme; method for forming ?-isomaltosylglucosaccharide; process for producing a cyclotetrasaccharide having the structure of cyclo{66)-?-D-glucopyranosyl-(163)-?-D-glucopyranosyl-Type: ApplicationFiled: May 9, 2007Publication date: February 7, 2008Applicant: KABUSHIKI KAISHA HAYASHIBARA SEIBUTSU KAGAKU KENKYUJOInventors: Michio Kubota, Keiji Tsusaki, Takanobu Higashiyama, Shigeharu Fukuda, Toshio Miyake
-
Publication number: 20080003651Abstract: The object of the present invention is to provide an ?-isomaltosylglucosaccharide-forming enzyme, process of the same, cyclotetrasaccharide, and saccharide composition comprising the saccharide which are obtainable by using the enzyme; and is solved by establishing an ?-isomaltosylglucosaccharide-forming enzyme which forms a saccharide, having a glucose polymerization degree of at least three and having both the ?-1,6 glucosidic linkage as a linkage at the non-reducing end and the ?-1,4 glucosidic linkage other than the linkage at the non-reducing end, by catalyzing the ?-glucosyl-transfer from a saccharide having a glucose polymerization degree of at least two and having the ?-1,4 glucosidic linkage as a linkage at the non-reducing end without substantially increasing the reducing power; ?-isomaltosyl-transferring method using the enzyme; method for forming ?-isomaltosylglucosaccharide; process for producing a cyclotetrasaccharide having the structure of cyclo{66)-?-D-glucopyranosyl-(163)-?-D-glucopyranosyl-Type: ApplicationFiled: May 9, 2007Publication date: January 3, 2008Applicant: KABUSHIKI KAISHA HAYASHIBARA SEIBUTSU KAGAKU KENKYUJOInventors: Michio Kubota, Keiji Tsusaki, Takanobu Higashiyama, Shigeharu Fukuda, Toshio Miyake
-
Patent number: 7241606Abstract: The object of the present invention is to provide an ?-isomaltosylglucosaccharide-forming enzyme, process of the same, cyclotetrasaccharide, and saccharide composition comprising the saccharide which are obtainable by using the enzyme; and is solved by establishing an ?-isomaltosylglucosaccharide-forming enzyme which forms a saccharide, having a glucose polymerization degree of at least three and having both the ?-1,6 glucosidic linkage as a linkage at the non-reducing end and the ?-1,4 glucosidic linkage other than the linkage at the non-reducing end, by catalyzing the ?-glucosyl-transfer from a saccharide having a glucose polymerization degree of at least two and having the ?-1,4 glucosidic linkage as a linkage at the non-reducing end without substantially increasing the reducing power; ?-isomaltosyl-transferring method using the enzyme; method for forming ?-isomaltosylglucosaccharide; process for producing a cyclotetrasaccharide having the structure of cyclo{?6)-?-D-glucopyranosyl-(1?3)-?-D-glucopyranosyl-Type: GrantFiled: July 25, 2001Date of Patent: July 10, 2007Assignee: Kabushiki Kaisha Hayashibara Seibutsu Kagaku KenkyujoInventors: Michio Kubota, Keiji Tsusaki, Takanobu Higashiyama, Shigeharu Fukuda, Toshio Miyake