Patents by Inventor Keiko Takihara

Keiko Takihara has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Method of preparing heart muscle cells and method of searching for remedy for heart diseases
    Publication number: 20080160553
    Abstract: The present invention relates to a method of preparing primary heart muscle cells, characterized by washing a fine fragmented heart tissue with a phosphate-buffered physiological saline to thereby eliminate non-heart muscle cells and then hemolyzing the heart tissue digested with a protease to thereby eliminate erythrocytes. According to this method, highly pure primary heart muscle cells can be conveniently obtained in a large amount from the heart of an animal embryo or newborn. By using the heart muscle thus prepared, apoptosis of heart muscle cells can be efficiently and highly sensitively detected. Thus, it is possible to efficiently screen candidate compounds for heart muscle cell apoptosis inhibitors, gp 130-mediated receptor agonists, heart muscle cell-protective signal enhancers, preventives and remedies for heart diseases.
    Type: Application
    Filed: February 7, 2008
    Publication date: July 3, 2008
    Applicant: Takeda Pharmaceutical Company Limited
    Inventors: Haruhide Kimura, Nobuyuki Koyama, Seiichi Tanida, Tadamitsu Kishimoto, Keiko Takihara, Hisao Hirota
  • Method of preparing heart muscle cells and method of searching for remedy for heart diseases
    Publication number: 20030175847
    Abstract: The present invention relates to a method of preparing primary heart muscle cells, characterized by washing a fine fragmented heart tissue with a phosphate-buffered physiological saline to thereby eliminate non-heart muscle cells and then hemolyzing the heart tissue digested with a protease to thereby eliminate erythrocytes. According to this method, highly pure primary heart muscle cells can be conveniently obtained in a large amount from the heart of an animal embryo or newborn. By using the heart muscle thus prepared, apoptosis of heart muscle cells can be efficiently and highly sensitively detected. Thus, it is possible to efficiently screen candidate compounds for heart muscle cell apoptosis inhibitors, gp 130-mediated receptor agonists, heart muscle cell-protective signal enhancers, preventives and remedies for heart diseases.
    Type: Application
    Filed: December 16, 2002
    Publication date: September 18, 2003
    Inventors: Haruhide Kimura, Nobuyuki Koyama, Seiichi Tanida, Tadamitsu Kishimoto, Keiko Takihara, Hisao Hirota