Patents by Inventor Keisuke Sekine
Keisuke Sekine has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20230357865Abstract: The present invention provides a marker gene capable of detecting the undifferentiated cells that remain or become included in a differentiated cell population. Undifferentiated cells present in a differentiated cell population are detected by using at least one gene selected from the group consisting of LINC00678 and PRDM14 as an undifferentiation marker. A method of detecting undifferentiated cells; a method of using the gene as an undifferentiation marker; and a kit for detecting undifferentiated cells. A method of selecting an undifferentiated cell clone is also provided.Type: ApplicationFiled: November 12, 2020Publication date: November 9, 2023Applicants: Public University Corporation Yokohama City University, Eiken Kagaku Kabushiki KaishaInventors: Hideki Taniguchi, Keisuke Sekine, Ryota Yasui
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Publication number: 20230257813Abstract: The present invention provides a sensitive and simple detection method, and a kit therefor, which detects residual undifferentiated human pluripotent stem cells in an intermediate product and/or a final product of a regenerative medical products, using an isothermal nucleic acid amplification method, and specifically, a LAMP method. A method for detecting presence or absence of undifferentiated cells in a non-undifferentiated cell population, wherein RNA derived from an undifferentiation marker gene exhibiting a significant difference in expression level between the undifferentiated cells and the non-undifferentiated cells in a sample containing a nucleic acid derived from the cell population of interest is detected by the isothermal nucleic acid amplification method.Type: ApplicationFiled: November 12, 2020Publication date: August 17, 2023Applicants: Public University Corporation Yokohama City University, Eiken Kagaku Kabushiki KaishaInventors: Hideki Taniguchi, Keisuke Sekine, Ryota Yasui, Atsuka Matsui
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Publication number: 20210254185Abstract: The present invention provides a marker gene capable of detecting the undifferentiated cells that remain or become included in a differentiated cell population. Undifferentiated cells present in a differentiated cell population are detected by using at least one gene selected from the group consisting of ESRG, VSNL1, THY1, SFRP2, SPP1, USP44 and CNMD as an undifferentiation marker. A method of detecting undifferentiated cells; a method of using the gene as an undifferentiation marker; and a kit for detecting undifferentiated cells. A method of selecting an undifferentiated cell clone is also provided.Type: ApplicationFiled: June 14, 2019Publication date: August 19, 2021Applicant: Public University Corporation Yokohama City UniversityInventors: Hideki Taniguchi, Keisuke Sekine
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Publication number: 20200362315Abstract: The present invention solves the following problems [1] to [3] found in conventional methods of preparing a three dimensional structure (organ primordium) by coculturing functional cells with umbilical cord-derived vascular endothelial cells and bone marrow-derived mesenchymal cells: [1] the quality of resultant organ primordia varies greatly depending on donors; [2] the growth capacities of cell sources are limited; and [3] it is difficult to secure immunocompatibility because cells are derived from different sources. An organ bud prepared from vascular cells, mesenchymal cells and tissue or organ cells, wherein each of the vascular cell, the mesenchymal cell and the tissue or organ cell has been induced from pluripotent stem cells. A method of preparing an organ bud, comprising culturing vascular cells, mesenchymal cells and tissue or organ cells in vitro, wherein each of the vascular cell, the mesenchymal cell and the tissue or organ cell has been induced from pluripotent stem cells.Type: ApplicationFiled: November 30, 2018Publication date: November 19, 2020Applicant: Public University Corporation Yokohama City UniversityInventors: Hideki Taniguchi, Takanori Takebe, Keisuke Sekine
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Patent number: 10647955Abstract: An operation isolator forms an aseptic space. An incubator is connected to the operation isolator, in which cells are stored and cultured. A storage chamber stores articles used in the operation isolator. In order to carry articles from the outside into the storage chamber, a decontamination pass-box is provided. The storage chamber and the operation isolator are directly or indirectly connected to each other.Type: GrantFiled: September 18, 2015Date of Patent: May 12, 2020Assignees: PUBLIC UNIVERSITY CORPORATION YOKOHAMA CITY UNIVERSITY, BIOMEDICA SOLUTIONS CO., LTD., SHIBUYA CORPORATIONInventors: Hideki Taniguchi, Takanori Takebe, Keisuke Sekine, Atsushi Nakao, Takashi Hiromatsu, Hironobu Sunayama, Tetsuya Nishimura
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Patent number: 10513685Abstract: Provided is a method that achieves control of embryoid body size and can induce differentiation in a state where the embryoid body size is controlled, by using a cell culture chamber having a plurality of microchambers formed therein. A culture method for causing differentiation of pluripotent mammalian cells uses a cell culture chamber (10) having a plurality of microchambers (11) formed on a culture surface. The cell culture chamber (10) has a culture surface formed of spaces in which the microchambers (11) have a space structure with a height of 10 ?m to 500 ?m and a bottom area of 100 ?m2 to 1 mm2. The culture method for causing differentiation of pluripotent mammalian cells includes culturing pluripotent mammalian cells to obtain a cell population at least partially differentiated into endoderm lineage cells, by using the cell culture chambers (10).Type: GrantFiled: March 23, 2011Date of Patent: December 24, 2019Assignees: CORNING INCORPORATED, PUBLIC UNIVERSITY CORPORATION YOKOHAMA CITY UNIVERSITYInventors: Yoko Itchoda, Go Tazaki, Masaya Hosoda, Motohiro Fukuda, Hideki Taniguchi, Yun-Wen Zheng, Keisuke Sekine
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Publication number: 20180352792Abstract: A revised technique for preparing human tissues and organs aiming at application to medical use is provided. With this technique, function per cell is greatly improved and great reduction in production cost is realized. A method of preparing an organ bud, comprising culturing vascular endothelial cells, mesenchymal cells and a tissue or organ cell in vitro in the presence of blood cells. Also provided are an organ bud prepared by the above method and a method of preparing a tissue or an organ using the organ bud. Using the same organ bud, there are provided a method of transplanting an organ bud, a method of regeneration or function recovery of a tissue or an organ, a method of preparing a non-human chimeric animal, and a method of evaluating a drug.Type: ApplicationFiled: December 19, 2016Publication date: December 13, 2018Applicant: PUBLIC UNIVERSITY CORPORATION YOKOHAMA CITY UNIVERSITYInventors: Hideki TANIGUCHI, Yunzhong NIE, Yun-Wen ZHENG, Keisuke SEKINE, Takanori TAKEBE
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Publication number: 20170306285Abstract: An operation isolator forms an aseptic space. An incubator is connected to the operation isolator, in which cells are stored and cultured. A storage chamber stores articles used in the operation isolator. In order to carry articles from the outside into the storage chamber, a decontamination pass-box is provided. The storage chamber and the operation isolator are directly or indirectly connected to each other.Type: ApplicationFiled: September 18, 2015Publication date: October 26, 2017Applicants: Public University Corporation Yokohama City University, Biomedica Solution Co., Ltd., SHIBUYA CORPORATIONInventors: Hideki TANIGUCHI, Takanori TAKEBE, Keisuke SEKINE, Atsushi NAKAO, Takashi HIROMATSU, Hironobu SUNAYAMA, Tetsuya NISHIMURA
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Patent number: 9789709Abstract: An image forming apparatus includes a sheet feeder, a carriage, a cutter, a cutter unit, and a controller. A first standby position and a second standby position are disposed on both ends of a range of movement of the carriage. The carriage does not contact the cutter unit at each of the first standby position and the second standby position. The controller is configured to control the carriage and the cutter unit to overlappingly move. In an image recording condition in which images are consecutively recorded in a plurality of pages on a sheet, the controller is configured to change a direction of movement of the carriage at a leading end of a page so that the direction of movement of the carriage is the same as a direction of movement of the cutter unit at a sheet cutting position at which the cutter cuts the sheet.Type: GrantFiled: April 12, 2016Date of Patent: October 17, 2017Assignee: RICOH COMPANY, LTD.Inventors: Soichi Saiga, Keisuke Sekine, Tomonori Naito, Shinichiro Naruse
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Publication number: 20170285002Abstract: It is intended to develop a technique that can reproduce a microenvironment of cancer tissue and to construct a novel drug discovery screening system of high precision. It is also intended to provide a method for reconstituting human cancer tissue using primary human cancer cells that retain the properties of human tumor. The present invention provides a reconstituted cancer organoid reproducing a cancer microenvironment. The present invention also provides a method for preparing a cancer organoid from cancer tissue, a xenograft prepared from the cancer organoid, a method for preparing the xenograft, a method for evaluating treatment resistance of cancer, a method for evaluating invasion or metastasis of cancer, a method for evaluating recurrence of cancer, and a method for conducting prognostic prediction of cancer.Type: ApplicationFiled: March 16, 2017Publication date: October 5, 2017Applicant: Public University Corporation Yokohama City UniversityInventors: Hideki TANIGUCHI, Yasuharu UENO, Takanori TAKEBE, Keisuke SEKINE, Ryo OKUDA
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Publication number: 20160297212Abstract: An image forming apparatus includes a sheet feeder, a carriage, a cutter, a cutter unit, and a controller. A first standby position and a second standby position are disposed on both ends of a range of movement of the carriage. The carriage does not contact the cutter unit at each of the first standby position and the second standby position. The controller is configured to control the carriage and the cutter unit to overlappingly move. In an image recording condition in which images are consecutively recorded in a plurality of pages on a sheet, the controller is configured to change a direction of movement of the carriage at a leading end of a page so that the direction of movement of the carriage is the same as a direction of movement of the cutter unit at a sheet cutting position at which the cutter cuts the sheet.Type: ApplicationFiled: April 12, 2016Publication date: October 13, 2016Applicant: RICOH COMPANY, LTD.Inventors: Soichi SAIGA, Keisuke SEKINE, Tomonori NAITO, Shinichiro NARUSE
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Publication number: 20130071932Abstract: Provided is a method that achieves control of embryoid body size and can induce differentiation in a state where the embryoid body size is controlled, by using a cell culture chamber having a plurality of microchambers formed therein. A culture method for causing differentiation of pluripotent mammalian cells uses a cell culture chamber (10) having a plurality of microchambers (11) formed on a culture surface. The cell culture chamber (10) has a culture surface formed of spaces in which the microchambers (11) have a space structure with a height of 10 ?m to 500 ?m and a bottom area of 100 ?m2 to 1 mm2. The culture method for causing differentiation of pluripotent mammalian cells includes culturing pluripotent mammalian cells to obtain a cell population at least partially differentiated into endoderm lineage cells, by using the cell culture chambers (10).Type: ApplicationFiled: March 23, 2011Publication date: March 21, 2013Applicants: Public University Corporation Yokohama City University, KURARAY Co., Ltd.Inventors: Yoko Itchoda, Go Tazaki, Masaya Hosoda, Motohiro Fukuda, Hideki Taniguchi, Yun-Wen Zheng, Keisuke Sekine