Abstract: The present invention relates to a method for screening a nucleic acid aptamer comprising: (a) contacting a target molecule immobilized on a solid phase support with a nucleic acid aptamer candidate; (b) collecting the nucleic acid aptamer candidate binding with the target molecule by a capillary electrophoresis; and (c) amplifying the nucleic acid aptamer candidate by PCR.

Abstract: The present invention relates to a method for screening a nucleic acid aptamer comprising: (a) contacting a target molecule immobilized on a solid phase support with a nucleic acid aptamer candidate; (b) collecting the nucleic acid aptamer candidate binding with the target molecule by a capillary electrophoresis; and (c) amplifying the nucleic acid aptamer candidate by PCR.

Abstract: A scaffold material for cell culturing using a nucleic acid aptamer that can be chemically synthesized, containing no animal-derived components, and having high biocompatibility is provided. A cell scaffold material containing an E-cadherin binding nucleic acid aptamer.

Abstract: [Problem] To provide a novel nucleic acid aptamer for a vascular endothelial growth factor receptor, said nucleic acid aptamer being useful for the diagnosis and treatment of various diseases associated with VEGFs that can regulate angiogenesis and receptors for the VEGFs, e.g., tumor angiogenesis, diabetic retina and chronic rheumatoid arthritis. [Solution] A nucleic acid aptamer characterized by comprising a nucleotide sequence represented by any one of SEQ ID NOs: 1 to 5, and also characterized by being capable of bonding to a human VEGF receptor specifically. In a preferred embodiment of the nucleic acid aptamer, a primer recognition sequence, a fluorescent label, or a biotin molecule, an avidin molecule, a streptavidin molecule or other specific binding tag peptide may be linked to the 5?- or 3?-terminal of the nucleic acid aptamer for the purpose of making it possible to detect the nucleic acid aptamer easily.

Abstract: [Problem] To provide a novel nucleic acid aptamer for a vascular endothelial growth factor receptor, said nucleic acid aptamer being useful for the diagnosis and treatment of various diseases associated with VEGFs that can regulate angiogenesis and receptors for the VEGFs, e.g., tumor angiogenesis, diabetic retina and chronic rheumatoid arthritis. [Solution] A nucleic acid aptamer characterized by comprising a nucleotide sequence represented by any one of SEQ ID NOs: 1 to 5, and also characterized by being capable of bonding to a human VEGF receptor specifically. In a preferred embodiment of the nucleic acid aptamer, a primer recognition sequence, a fluorescent label, or a biotin molecule, an avidin molecule, a streptavidin molecule or other specific binding tag peptide may be linked to the 5?- or 3?-terminal of the nucleic acid aptamer for the purpose of making it possible to detect the nucleic acid aptamer easily.

Abstract: A solution including a single-stranded nucleic acid (10) having a target base (11) related to SNP or the like is mixed with a solution including two kinds of single-stranded detecting nucleic acids (20a) and (20b) complementary to partial sequences that sandwich the target base (11) between them to hybridize the target nucleic acid (10) and the detecting nucleic acids (20a) and (20b). Thus, a gap part (21) is intentionally formed at a position opposed to the target base (11). Then, a receptor molecule (30) having hydrogen bonding characteristics and fluorescence emitting characteristics is inserted to the gap part (21) as a hydrophobic space. Then, the change of fluorescent strength of the receptor molecule (30) depending on the difference of the target base (11) is detected to detect a single nucleotide substitution.