Abstract: A method is disclosed for analyzing keratinized tissue, particularly fingernails, of a subject to diagnose osteoporosis and bone fracture risk. A Raman spectrum of a sample of keratinized tissue is generated. Broad spectral background features of the spectrum are removed, preferably by using Fourier transform analysis. Peak heights of Raman features of interest, particularly the S—S bond of cystine, are measured. These peak height measurements are normalized using reference peak heights of Raman features that are invariant between normal and osteoporotic subjects, such as the CH2 bending peak.

Abstract: A programmable Raman transducer is disclosed for detecting the presence or absence of a preselected compound in a sample. The transducer, in a preferred embodiment, includes a laser source for generating laser light for illuminating the sample. Collector optics, absent a spatial filter, are used for collecting Raman-scattered light from the sample. A detector generates spectral data from the Raman-scattered light, and a digital processor compares the spectral data to a database of spectral data on selected compounds, including the preselected compound to generate a binary signal indicating presence or absence of the preselected compound.

Abstract: An apparatus is described for the real-time identification of one or more selected components of a target material. In one embodiment, an infrared spectrometer and a separate Raman spectrometer are coupled to exchange respective spectral information of the target material preferably normalized and presented in a single graph. In an alternative embodiment, both an infrared spectrometer and a Raman spectrometer are included in a single instrument and a common infrared light source is used by both spectrometers. In another embodiment, a vibrational spectrometer and a stoichiometric spectrometer are combined in a single instrument and are coupled to exchange respective spectral information of the target material and to compare the spectral information against a library of spectra to generate a real-time signal if a selected component is present in the target material.

Abstract: A method of reporting a binding event having increased sensitivity and multiplex capabilities. A reporter supra-nanoparticle assembly is provided that has an inner core made of a polymeric material, a coating on the inner core made of a polymeric material and a plurality of reporter nanoparticles, and a first active group on the surface of the coating.

Abstract: A method is disclosed for analyzing keratinized tissue, particularly fingernails, of a subject to diagnose osteoporosis and bone fracture risk. A Raman spectrum of a sample of keratinized tissue is generated. Broad spectral background features of the spectrum are removed, preferably by using Fourier transform analysis. Peak heights of Raman features of interest, particularly the S—S bond of cystine, are measured. These peak height measurements are normalized using reference peak heights of Raman features that are invariant between normal and osteoporotic subjects, such as the CH2 bending peak.

Abstract: Echelle gratings and microelectromechanical system (MEMS) digital micromirror device (DMD) detectors are used to provide rapid, small, and highly sensitive spectrometers. The new spectrometers are particularly useful for laser induced breakdown and Raman spectroscopy, but could generally be used with any form of emission spectroscopy. The new spectrometers have particular applicability in the detection of improvised explosive devices.

Abstract: An apparatus is described for the real-time identification of one or more selected components of a target material. In one embodiment, an infrared spectrometer and a separate Raman spectrometer are coupled to exchange respective spectral information of the target material preferably normalized and presented in a single graph. In an alternative embodiment, both an infrared spectrometer and a Raman spectrometer are included in a single instrument and a common infrared light source is used by both spectrometers. In another embodiment, a vibrational spectrometer and a stoichiometric spectrometer are combined in a single instrument and are coupled to exchange respective spectral information of the target material and to compare the spectral information against a library of spectra to generate a real-time signal if a selected component is present in the target material.

Abstract: A Raman spectroscopy technique allows an analyte, a paramagnetic particle, and a spectral enhancement particle to combine in solution and for the combination product to be localized by a magnetic field for analysis. The spectral enhancement particle may be comprised of an active SERS metal particle with or without a material coating. The spectral enhancement particle may function as a reporter for the presence of the analyte or merely increase the magnitude of the Raman spectrum of the analyte. The technique is applicable to both immunoassays and chemical assays. Multiple spectral enhancement particle reporters may be measured in a single assay that can detect multiple analytes using the SERS effect.

Abstract: A system, method and apparatus for taking a Raman spectrum of a sample is disclosed. In one embodiment, for example, an integrated Raman spectrometer is provided. In another embodiment, a portable Raman spectrometer is provided. In another embodiment, a Raman spectrometer is provided comprising a collimated beam tube for transmitting excitation radiation to an external optical system, such as a microscope, a telescope or a camera lens. In another embodiment, a method for correcting a Raman spectrum for background interference is provided. In yet another embodiment, a method for rejecting fluorescence in a Raman spectrometer is provided. A chemical reactor comprising a built-in Raman detector for monitoring a chemical reaction in a reaction chamber of the reactor is also provided.

Abstract: An assay method and kit for detecting a chemical. The method and kit utilize a metal surface capable of surface enhanced Raman Scattering. The metal surface may be provided in the form of one or more nanoparticles, to increase the surface enhanced Raman Scattering capability of the metal surface. The nanoparticles may be treated with one or more additives to further enhance or maintain the surface enhanced Raman Scattering capability of the nanoparticles.