Abstract: Non-human animal cells and non-human animals comprising a humanized TTR locus comprising a beta-slip mutation and methods of using such non-human animal cells and non-human animals are provided. Non-human animal cells or non-human animals comprising a humanized TTR locus comprising a beta-slip mutation express a human transthyretin protein or a chimeric transthyretin protein, fragments of which are from human transthyretin. Methods are provided for using such non-human animals comprising a humanized TTR locus to assess in vivo efficacy of human-TTR-targeting reagents such as nuclease agents designed to target human TTR.

Abstract: Tau reporter compositions, tau reporter cells, and tau reporter animals are provided that comprise a four-repeat (4R) tau isoform linked to a first reporter protein and a three-repeat (3R) tau isoform linked to a second reporter protein that is different from the first reporter protein. Methods are provided for making such tau reporter cells and tau reporter animals and for using such tau reporter cells and tau reporter animals for assessing the activity of tau-targeting reagents.

Abstract: Non-human animal cells and non-human animals comprising a humanized TTR locus comprising a beta-slip mutation and methods of using such non-human animal cells and non-human animals are provided. Non-human animal cells or non-human animals comprising a humanized TTR locus comprising a beta-slip mutation express a human transthyretin protein or a chimeric transthyretin protein, fragments of which are from human transthyretin. Methods are provided for using such non-human animals comprising a humanized TTR locus to assess in vivo efficacy of human-TTR-targeting reagents such as nuclease agents designed to target human TTR.

Abstract: A gutter system is described which combines a leaf screen, rain gutter, drip edge and soffit vent into a unitary, molded plastic structure. The gutter system forms the fascia along a roof eaves. The gutter fascia can be molded with a wood grained texture or left smooth, and can be molded of any desired color. Because it is molded from a flexible, plastic material, the gutter can be folded flat, allowing multiple sections to be stacked and packed into a relatively small shipping carton.

Abstract: A gutter system is described which combines a leaf screen, rain gutter, drip edge and soffit vent into a unitary, molded plastic structure. The gutter system forms the fascia along a roof eaves. The gutter fascia can be molded with a wood grained texture or left smooth, and can be molded of any desired color. Because it is molded from a flexible, plastic material, the gutter can be folded flat, allowing multiple sections to be stacked and packed into a relatively small shipping carton.