Abstract: A substance identification method includes combining substances into four or more intermediate subpools in wells of a subpool plate and repooling the intermediate subpools into a number of final screening pools based on a repooling design providing the subpooled substances in at least three different final screening pools. The repooling design determines coordinates locating well positions for the substances. Another substance identification method includes using a two-dimensional array of wells arranged in rows and a number of columns that is at least 1.5 times the rows. Substances in the wells are combined into a number of screening pools. Individual screening pools include substances from wells having a row identifier in common with one other well. A pooling design provides the pooled substances in two different screening pools. The pooling design determines coordinates locating well positions for the substances.

Abstract: This application pertains to construction of pooled biological material such as DNA, RNA, proteins and the like that are able to be screened by a wide variety of methods such as PCR (Polymerase Chain Reaction), DNA/DNA hybridization, DNA/RNA hybridization, RNA/RNA hybridization, single strand DNA probing, protein/protein hybridization and a wide variety of additional methods. Our new method for construction of pools and superpools for screening differs in that the complete set is systematically divided into a variety of smaller subsets which are then re-pooled to make the final screening pools. This pooled material can be from individual samples or a population of samples. In order to reduce the analysis time, materials and expense, the pooling of high resolution small pools in a matrix allows for a lower number of user experiments to have higher resolution (as if the researcher had analyzed the complete set of small pools).

Abstract: This application pertains to construction of pooled biological material such as DNA, RNA, proteins and the like that are able to be screened by a wide variety of methods such as PCR (Polymerase Chain Reaction), DNA/DNA hybridization, DNA/RNA hybridization, RNA/RNA hybridization, single strand DNA probing, protein/protein hybridization and a wide variety of additional methods. Our new method for construction of pools and superpools for screening differs in that the complete set is systematically divided into a variety of smaller subsets which are then re-pooled to make the final screening pools. This pooled material can be from individual samples or a population of samples. In order to reduce the analysis time, materials and expense, the pooling of high resolution small pools in a matrix allows for a lower number of user experiments to have higher resolution (as if the researcher had analyzed the complete set of small pools).

Abstract: Embodiments of a remediation reactor and mixer/contactor blade for the reactor are shown and described, the reactor (11) being for containing a liquid slurry, suspension or settled bed of solid particles containing microorganisms. Inside the vessel is a supply conduit and at least one generally horizontal stirrer blade (27) in fluid connection with the supply conduit. The stirrer blade is a mixer/contactor which has a leading side having openings through which fluid may pass. The stirrer blade rotates in the vessel, and this rotation is made easier by the hydraulic forces of fluid flowing out from the stirrer blade. The flowing fluid creates a fluidization zone in the slurry, suspension or settled bed at or near the leading edge of the stirrer blade. The fluidization zone is less dense than the rest of the sediment bed, and the stirrer blade tends to rotate into the fluidization zone.

Abstract: Embodiments of a slurry reactor and slurry mixer/contactor are shown and described. Each embodiment of the reactor includes a vessel for containing a liquid slurry or suspension of particles and microorganisms. In the inside of the vessel, generally along its centerline, is a vertical conduit extending from near the top to near the bottom. At the bottom of the vertical conduit is at least one generally horizontal, hollow, stirrer blade in fluid connection with the vertical conduit. The stirrer blade is a mixer/contactor which has openings near its leading edge so fluid may pass therethrough. The stirrer blade may rotate around the vertical conduit if the conduit is fixed, or the conduit, with the stirrer blade fixed to it, may rotate around in the vessel. In any event, the rotation of the stirrer blade is caused by the hydraulic forces of fluid flowing out from the stirrer blade. The flowing fluid creates a fluidization zone in the slurry sediment bed at the leading edge of the stirrer.

Abstract: A dynamic bed reactor is disclosed in which a compressible open cell foam matrix is periodically compressed and expanded to move a liquid or fluid through the matrix. In preferred embodiments, the matrix contains an active material such as an enzyme, biological cell, chelating agent, oligonucleotide, adsorbent or other material that acts upon the liquid or fluid passing through the matrix. The active material may be physically immobilized in the matrix, or attached by covalent or ionic bonds. Microbeads, substantially all of which have diameters less than 50 microns, can be used to immobilize the active material in the matrix and further improve reactor efficiency. A particularly preferred matrix is made of open cell polyurethane foam, which adsorbs pollutants such as polychlorophenol or o-nitrophenol.

Abstract: An apparatus for heating and cooling which features a unique energy recovery ventilation apparatus and method. During energy recovery ventilation, a cycling damper controls the flow of warm and cool air to maintain the inside air temperature when inside air is cycled to the outside. The outside air is then warmed or cooled when drawn to the inside.