Abstract: Systems, methods and algorithms for automatically performing optimization of an electroporation system. A system according to the present invention typically includes a cuvette holding assembly configured to hold a plurality of electroporation cuvettes, wherein each cuvette includes a first and second electrode, and a shocking chamber configured to hold the cuvette holding assembly, the chamber having a commutator assembly configured to provide an electrical contact to the first electrode of each of the plurality of cuvettes in turn. The system also typically includes a control system communicably coupled to the shocking chamber, wherein the control system controls the commutator to automatically contact the first electrode of each cuvette in an order and to provide a potential across the cuvette electrodes when contact is made.

Abstract: A shocking chamber for performing electroporation is constructed in the form of a base and a hinged lid which when lowered forms an enclosure with the base that fully encloses a cuvette and impresses a high voltage across the cuvette, the voltage connection becoming disengaged upon the simple raising of the lid. The base contains two pairs of electrical leads, one pair engaging the cuvette with spring-loaded contacts that provide electrical connections to the cuvette while helping to secure the cuvette in place, and the other pair joined to high-voltage terminals. A shunt built into the lid bridges the two pairs of leads in the base when the lid is closed and pivots out of the way to clear all leads when the lid is opened. These and other features of the construction provide the user with a safe and secure means of forming the high-voltage electrical connections used in electroporation in an apparatus that can be operated with one hand.

Abstract: A shocking chamber for performing electroporation is constructed in the form of a base and a hinged lid which when lowered forms an enclosure with the base that fully encloses a cuvette and impresses a high voltage across the cuvette, the voltage connection becoming disengaged upon the simple raising of the lid. The base contains two pairs of electrical leads, one pair engaging the cuvette with spring-loaded contacts that provide electrical connections to the cuvette while helping to secure the cuvette in place, and the other pair joined to high-voltage terminals. A shunt built into the lid bridges the two pairs of leads in the base when the lid is closed and pivots out of the way to clear all leads when the lid is opened. These and other features of the construction provide the user with a safe and secure means of forming the high-voltage electrical connections used in electroporation in an apparatus that can be operated with one hand.

Abstract: Systems, methods and algorithms for automatically performing optimization of an electroporation system. A system according to the present invention typically includes a cuvette holding assembly configured to hold a plurality of electroporation cuvettes, wherein each cuvette includes a first and second electrode, and a shocking chamber configured to hold the cuvette holding assembly, the chamber having a commutator assembly configured to provide an electrical contact to the first electrode of each of the plurality of cuvettes in turn. The system also typically includes a control system communicably coupled to the shocking chamber, wherein the control system controls the commutator to automatically contact the first electrode of each cuvette in an order and to provide a potential across the cuvette electrodes when contact is made.

Abstract: A vacuum aspirator apparatus uses circulating water from a reservoir while avoiding or reducing temperature rise in the circulating water. The apparatus consists of a reservoir with aspiration and cooling circuits, both of which are external to the reservoir, the aspiration circuit containing one or more aspirators with vacuum ports at which a vacuum can be drawn.

Abstract: A non-dispersive infrared gas analyzer especially adapted for measuring the concentrations of HC, CO and CO.sub.2 in a vehicle exhaust includes a sample chamber for holding a sample gas, a radiation emitter for directing a beam through the chamber and a detector for indicating the amount of radiation absorbed. A plurality of filters each of which transmit radiation at an absorption band of a gas component to be detected are alternatingly positioned in the radiation path to produce a time-multiplexed signal having concentration information for all gases. The time-multiplexed detector output is corrected for gain and offset errors "on-the-fly", without requiring demultiplexing, through the use of an analog multiplier circuit. The correction factors are themselves determined without demultiplexing the detector output signal.

Abstract: A non-dispersive infrared gas analyzer especially adapted for measuring the concentrations of HC, CO and CO.sub.2 in a vehicle exhaust includes a sample chamber for holding a sample gas, a radiation emitter for directing a beam through the chamber and a detector for indicating the amount of radiation absorbed. A plurality of filters, each of which transmit radiation at an absorption band of a gas component to be detected, are alternatingly positioned in the radiation path to produce a time-multiplexed signal having concentration information for all gases. A calibration assembly is provided which includes a plurality of calibration cells selectively positionable in the radiation path in order to provide an internal calibration standard for two-point calibration of each gas channel. The cells contain one or more of the gas components to be measured at concentrations and pressures predetermined to compensate for the difference in optical path length between the calibration cells and the sample chamber.

Abstract: Apparatus for inserting and removing several filters from an optical path and for determining at all times the instantaneous positional relationship of each filter with respect to the optical path includes a set of filters mounted on a carrier which is attached to a resonant piezoelectric system, and further includes reference tracks also located on the carrier and extending in the direction of the oscillatory motion and including a sequence of binary indicia progressing in the direction of the oscillatory motion. The reference tracks are read optically to produce a series of binary electrical signals which are processed to yield the instantaneous position of the carrier and filters with respect to a fixed optical path.