Abstract: An image system for detecting chemiluminescence in a sample uses a highly binned, short exposure initial image to calculate the exposure time for a final image of the sample. After calculation of the exposure time, at least two final images are taken, with saturated pixels removed and replaced in a first image with corresponding unsaturated pixels from a second image. The corresponding pixels are adjusted to reflect the different intensity levels between the first and second images, and the first image becomes the final image reflecting the detected chemiluminescence.

Abstract: Methods, apparatuses, and systems for imaging biological/chemical samples are provided. A calibrated imaging system can allow a user to obtain an optimal focus setting (position) for any effective distance (e.g. a zoom setting). The optimal focus can be determined from a functional approximation that defines a relationship between effective distance and focus setting. A user can input a size, and an imaging system can determine the appropriate effective distance and focus. An imaging system can also determine a size based on any effective distance. A flat-field correction can also be determined for any effective distance or size.

Abstract: The present invention provides a transmissive density and/or reflective density reference step tablet and methods of using thereof for calibrating a densitometer or the like.

Abstract: An image system for detecting chemiluminescence in a sample uses a highly binned, short exposure initial image to calculate the exposure time for a final image of the sample. After calculation of the exposure time, at least two final images are taken, with saturated pixels removed and replaced in a first image with corresponding unsaturated pixels from a second image. The corresponding pixels are adjusted to reflect the different intensity levels between the first and second images, and the first image becomes the final image reflecting the detected chemiluminescence.

Abstract: Methods, apparatuses, and systems for imaging biological/chemical samples are provided. A calibrated imaging system can allow a user to obtain an optimal focus setting (position) for any effective distance (e.g. a zoom setting). The optimal focus can be determined from a functional approximation that defines a relationship between effective distance and focus setting. A user can input a size, and an imaging system can determine the appropriate effective distance and focus. An imaging system can also determine a size based on any effective distance. A flat-field correction can also be determined for any effective distance or size.

Abstract: The optical imaging of two-dimensional solute zone arrays in electrophoresis gels is corrected for nonuniformities in the optical system such as those arising from the light source or from light dispersion underneath the gel. The correction is achieved by the use of a reference plate that responds to a light source uniformly along its length and width by being either uniformly light absorptive or uniformly light transmissive, or by emitting light upon excitation. Thus, any nonuniformities or deviations in the image of the reference plate arise only from nonuniformities or deviations within the optical system. Analogous corrections are made in other two-dimensional assay images, such as microarrays and microtiter plates.