Abstract: Frac sand from a well completion operation is transported to a recycling facility by truck. The frac sand is passed into a washing facility where is it separated from frac sand carrier residue, clays, drilling mud and metal particles. The wash water used in the washing facility is settled and re-used. Mud from the settled wash water is removed and re-used in drilling operations. The frac sand is removed from the washing facility by an inclined dehydration screw, and discharged into a counterflow hot air drying facility. Dust from the drying facility is collected and removed. Hot frac sand from the drying facility is passed through a chiller, and collected. The collected material is sized, and stored for re-use.

Abstract: The invention is directed to methods for the non-radioactive labeling, detection, quantitation and isolation of nascent proteins translated in a cellular or cell-free translation system. tRNA molecules are misaminoacylated with non-radioactive markers which may be non-native amino acids, amino acid analogs or derivatives, or substances recognized by the protein synthesizing machinery. Markers may comprise cleavable moieties, detectable labels, reporter properties wherein markers incorporated into protein can be distinguished from unincorporated markers, or coupling agents which facilitate the detection and isolation of nascent protein from other components of the translation system. The invention also comprises proteins prepared using misaminoacylated tRNAs which can be utilized in pharmaceutical compositions for the treatment of diseases and disorders in humans and other mammals, and kits which may be used for the detection of diseases and disorders.

Abstract: Frac sand from a well completion operation is transported to a recycling facility by truck. The frac sand is passed into a washing facility where is it separated from frac sand carrier residue, clays, drilling mud and metal particles. The wash water used in the washing facility is settled and re-used. Mud from the settled wash water is removed and re-used in drilling operations. The frac sand is removed from the washing facility by an inclined dehydration screw, and discharged into a counterflow hot air drying facility. Dust from the drying facility is collected and removed. Hot frac sand from the drying facility is passed through a chiller, and collected. The collected material is sized, and stored for re-use.