Abstract: Embodiments in accordance with the present disclosure are directed to methods and apparatuses used for evaluating a protein in a sample using proteolysis and translocation. An example method includes isolating a single protein from a sample in a chamber having at least a first well and a second well separated from the first well by a membrane with a nanopore, the nanopore providing fluidic communication between the first and second wells. The single protein is cleaved into a plurality of peptide fragments via exposure to a protease in the first well, and translocated through the nanopore by applying an electric potential to the nanopore in the chamber after cleavage of the single protein by the protease. The method further includes detecting events indicative of the translocations of the plurality of peptide fragments through the nanopore and to the second well.

Abstract: A method is provided for treating a patient with a medical condition that involves angiogenesis or HIF-1 overexpression with a compound having the structure of formula (I) wherein Z, x, y, and R1 through R11 are as defined herein.

Abstract: A device and method for use with a centrifuge for fragmenting solute or particulate material contained in a liquid sample are disclosed. The device includes a substrate adapted to be supported within a centrifuge tube, and providing a microchannel defining a plurality of shear regions. Material contained in a liquid sample applied to the device, with such supported in a centrifuge tube within a centrifuge, is fragmented by shearing as the sample is forced successively through the plurality of shear regions in the microchannel, when the selected centrifugal force applied to the tube.

Abstract: The present invention relates, inter alia, to compounds that bind tubulin and exhibit anti-mitotic properties and to methods of using such compounds to inhibit abnormal cell mitosis and, in particular, to inhibit tumor cell growth. In addition, methods are presented of treating mammalian diseases associated with undesired and uncontrolled angiogenesis.