Patents by Inventor Keith Leonard

Keith Leonard has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 11236305
    Abstract: Methods of producing stem cell conditioned media to treat mammalian injuries or insults. In at least one embodiment of a method for isolating non-endothelial adipocyte-depleted stromal cells of the present disclosure, the method comprises, comprising dissociating subcutaneous adipose tissue isolated from a mammal into a cell suspension, removing adipocytes from said cell suspension, resulting in a non-endothelial adipocyte-depleted cell suspension, and culturing the non-endothelial adipocyte-depleted cell suspension in a media containing growth factors VEGF, bFGF, EGF, and IGF, such that a mixed population of cells comprising a first population of further differentiated non-endothelial adipocyte-depleted CD34+/VE-cadherin? cells and a second population of further differentiated non-endothelial adipocyte-depleted CD34+/VE-cadherin+ cells are obtained and expanded.
    Type: Grant
    Filed: February 11, 2019
    Date of Patent: February 1, 2022
    Inventor: Keith Leonard March
  • Publication number: 20200308547
    Abstract: Stem cell conditioned media and methods of producing the same to treat mammalian injuries or insults. In at least one embodiment of a method of producing a stem cell conditioned media of the present disclosure, the method comprises the steps of culturing at least one stem cell in a first cell culture medium, replacing some or all of the first cell culture medium with a second cell culture medium and further culturing the at least one stem cell in the second cell culture medium, and collecting a quantity of the second cell culture medium after a culture duration, wherein the quantity of the second cell culture medium contains a cell culture byproduct effective to treat a mammalian insult or injury. A stem cell culture supernatant containing at least one factor capable of exerting effective neuroprotection to treat a mammalian neural injury or insult.
    Type: Application
    Filed: June 16, 2020
    Publication date: October 1, 2020
    Applicant: NeuroFx, Inc.
    Inventors: Keith Leonard March, Brian H. Johnstone, Yansheng Du
  • Patent number: 10683482
    Abstract: Methods of producing stem cell conditioned media to treat mammalian injuries or insults. In at least one embodiment of a method of producing a stem cell conditioned media of the present disclosure, the method comprises the steps of culturing at least one stem cell in a first cell culture medium, replacing some or all of the first cell culture medium with a second cell culture medium and further culturing the at least one stem cell in the second cell culture medium, and collecting a quantity of the second cell culture medium after a culture duration, wherein the quantity of the second cell culture medium contains a cell culture byproduct effective to treat a mammalian insult or injury.
    Type: Grant
    Filed: January 2, 2018
    Date of Patent: June 16, 2020
    Assignee: NeuroFx, Inc.
    Inventors: Keith Leonard March, Brian H. Johnstone, Yansheng Du
  • Publication number: 20190169573
    Abstract: Methods of producing stem cell conditioned media to treat mammalian injuries or insults. In at least one embodiment of a method for isolating non-endothelial adipocyte-depleted stromal cells of the present disclosure, the method comprises, comprising dissociating subcutaneous adipose tissue isolated from a mammal into a cell suspension, removing adipocytes from said cell suspension, resulting in a non-endothelial adipocyte-depleted cell suspension, and culturing the non-endothelial adipocyte-depleted cell suspension in a media containing growth factors VEGF, bFGF, EGF, and IGF, such that a mixed population of cells comprising a first population of further differentiated non-endothelial adipocyte-depleted CD34+/VE?cadherin? cells and a second population of further differentiated non-endothelial adipocyte-depleted CD34+/VE?cadherin+ cells are obtained and expanded.
    Type: Application
    Filed: February 11, 2019
    Publication date: June 6, 2019
    Inventor: Keith Leonard March
  • Publication number: 20180290096
    Abstract: A non-extruded filter article, including: an activated carbon honeycomb substrate having a plurality of flow-through channels and porous walls, and the activated carbon substrate comprises a carbon in from 90 to 99.9 wt. % of the article, and the porous walls have a percentage porosity of from 40% to 65%. Also disclosed is a non-extrusion method of making the article and a method of using the article.
    Type: Application
    Filed: June 8, 2018
    Publication date: October 11, 2018
    Inventors: Keith Leonard House, Benedict Yorke Johnson
  • Patent number: 10046264
    Abstract: A non-extruded filter article, including: an activated carbon honeycomb substrate having a plurality of flow-through channels and porous walls, and the activated carbon substrate comprises a carbon in from 90 to 99.9 wt. % of the article, and the porous walls have a percentage porosity of from 40% to 65%. Also disclosed is a non-extrusion method of making the article and a method of using the article.
    Type: Grant
    Filed: September 30, 2015
    Date of Patent: August 14, 2018
    Assignee: Corning Incorporated
    Inventors: Keith Leonard House, Benedict Yorke Johnson
  • Publication number: 20180187159
    Abstract: Methods of producing stem cell conditioned media to treat mammalian injuries or insults. In at least one embodiment of a method of producing a stem cell conditioned media of the present disclosure, the method comprises the steps of culturing at least one stem cell in a first cell culture medium, replacing some or all of the first cell culture medium with a second cell culture medium and further culturing the at least one stem cell in the second cell culture medium, and collecting a quantity of the second cell culture medium after a culture duration, wherein the quantity of the second cell culture medium contains a cell culture byproduct effective to treat a mammalian insult or injury.
    Type: Application
    Filed: January 2, 2018
    Publication date: July 5, 2018
    Applicant: NeuroFx, Inc.
    Inventors: Keith Leonard March, Brian H. Johnstone, Yansheng Du
  • Patent number: 9908803
    Abstract: Transparent glass sheets having increased mechanical strength include an inner layer surrounded by surface compressive layers wherein the difference of the coefficient of thermal expansion of the inner layer and the surface compressive layer is greater than 50×10?7° C.?1 and wherein the surface compressive layer has a compressive stress of at least about 300 MPa.
    Type: Grant
    Filed: August 2, 2016
    Date of Patent: March 6, 2018
    Assignee: Corning Incorporated
    Inventors: Dana Craig Bookbinder, Keith Leonard House, Pushkar Tandon
  • Patent number: 9908098
    Abstract: A flow-through filter article, as defined herein, including: a honeycomb and at least a portion of the honeycomb having a surface modification comprising: a first adsorbent comprising an activated carbon; a second adsorbent for a volatile organic compound, as defined herein; and a binder. Also disclosed is a method of making and using the filter article.
    Type: Grant
    Filed: September 30, 2015
    Date of Patent: March 6, 2018
    Assignee: Corning Incorporated
    Inventors: Keith Leonard House, Zhihui Lv
  • Patent number: 9856455
    Abstract: Methods of producing stem cell conditioned media to treat mammalian injuries or insults. In at least one embodiment of a method of producing a stem cell conditioned media of the present disclosure, the method comprises the steps of culturing at least one stem cell in a first cell culture medium, replacing some or all of the first cell culture medium with a second cell culture medium and further culturing the at least one stem cell in the second cell culture medium, and collecting a quantity of the second cell culture medium after a culture duration, wherein the quantity of the second cell culture medium contains a cell culture byproduct effective to treat a mammalian insult or injury.
    Type: Grant
    Filed: November 23, 2015
    Date of Patent: January 2, 2018
    Assignee: NeuroFx, Inc.
    Inventors: Keith Leonard March, Brian H. Johnstone, Yansheng Du
  • Publication number: 20170211045
    Abstract: Adipose tissue-derived stromal cells and methods of isolating and using the same. In at least one embodiment of isolated adipose tissue-derived stromal cells of the present disclosure, the cells are isolated by performing adipose tissue resection or suction on a mammalian patient, dissecting tissue obtained from said tissue resection or suction and dissociating said tissue into a cell suspension, removing adipocytes from the cell suspension, culturing the adipocyte-depleted cell suspension in EGM-2-MV media, and isolating adipose tissue-derived stromal cells secreting vascular endothelial growth factor (VEGF), hepatocyte growth factor (HGF), and granulocyte-colony stimulating factor (G-CSF).
    Type: Application
    Filed: January 1, 2017
    Publication date: July 27, 2017
    Inventor: Keith Leonard March
  • Publication number: 20160340221
    Abstract: Transparent glass sheets having increased mechanical strength include an inner layer surrounded by surface compressive layers wherein the difference of the coefficient of thermal expansion of the inner layer and the surface compressive layer is greater than 50×107° C.?1 and wherein the surface compressive layer has a compressive stress of at least about 300 MPa.
    Type: Application
    Filed: August 2, 2016
    Publication date: November 24, 2016
    Inventors: Dana Craig Bookbinder, Keith Leonard House, Pushkar Tandon
  • Publication number: 20160307368
    Abstract: A compressed format provides more efficient storage for light-field pictures. A specialized player is configured to project virtual views from the compressed format. According to various embodiments, the compressed format and player are designed so that implementations using readily available computing equipment are able to project new virtual views from the compressed data at rates suitable for interactivity. Virtual-camera parameters, including but not limited to focus distance, depth of field, and center of perspective, may be varied arbitrarily within the range supported by the light-field picture, with each virtual view expressing the parameter values specified at its computation time. In at least one embodiment, compressed light-field pictures containing multiple light-field images may be projected to a single virtual view, also at interactive or near-interactive rates.
    Type: Application
    Filed: April 12, 2016
    Publication date: October 20, 2016
    Inventors: Kurt Akeley, Nikhil Karnad, Keith Leonard, Colvin Pitts
  • Patent number: 9434633
    Abstract: Transparent glass sheets having increased mechanical strength include an inner layer surrounded by surface compressive layers wherein the difference of the coefficient of thermal expansion of the inner layer and the surface compressive layer is greater than 50×10?7° C.?1 and wherein the surface compressive layer has a compressive stress of at least about 300 MPa.
    Type: Grant
    Filed: June 28, 2011
    Date of Patent: September 6, 2016
    Assignee: CORNING INCORPORATED
    Inventors: Dana Craig Bookbinder, Keith Leonard House, Pushkar Tandon
  • Publication number: 20160158289
    Abstract: The present disclosure provides methods for treating patients with acute or chronic lung disease or injury to the lungs including injury caused by exposure to cigarette smoke other irritants or another cause of pulmonary distress. Typical conditions that can be treated include conditions that cause inflammation in the lung or the death of lung endothelial cells. Treatment of other conditions such as compromised bone marrow function and cachexia can also be treated by the inventive methods disclosed herein. These methods including contacting Adipose Stem Cells (ASC) or media conditioned by contact with ASC (ASC-CM) or various factors secreted by the same including the media or components of the media with lung tissue and cells. In some instances the ASC used is harvested from the patient's own adipose tissue while in other instances the source is an exogenous donor.
    Type: Application
    Filed: February 11, 2016
    Publication date: June 9, 2016
    Inventors: Keith Leonard March, Irina Petrache
  • Publication number: 20160145576
    Abstract: Methods of producing stem cell conditioned media to treat mammalian injuries or insults. In at least one embodiment of a method of producing a stem cell conditioned media of the present disclosure, the method comprises the steps of culturing at least one stem cell in a first cell culture medium, replacing some or all of the first cell culture medium with a second cell culture medium and further culturing the at least one stem cell in the second cell culture medium, and collecting a quantity of the second cell culture medium after a culture duration, wherein the quantity of the second cell culture medium contains a cell culture byproduct effective to treat a mammalian insult or injury.
    Type: Application
    Filed: November 23, 2015
    Publication date: May 26, 2016
    Inventors: Keith Leonard March, Brian H. Johnstone, Yansheng Du
  • Publication number: 20160096163
    Abstract: A flow-through filter article, as defined herein, including: a honeycomb and at least a portion of the honeycomb having a surface modification comprising: a first adsorbent comprising an activated carbon; a second adsorbent for a volatile organic compound, as defined herein; and a binder. Also disclosed is a method of making and using the filter article.
    Type: Application
    Filed: September 30, 2015
    Publication date: April 7, 2016
    Inventors: Keith Leonard House, Zhihui Lv
  • Publication number: 20160096137
    Abstract: A non-extruded filter article, including: an activated carbon honeycomb substrate having a plurality of flow-through channels and porous walls, and the activated carbon substrate comprises a carbon in from 90 to 99.9 wt. % of the article, and the porous walls have a percentage porosity of from 40% to 65%. Also disclosed is a non-extrusion method of making the article and a method of using the article.
    Type: Application
    Filed: September 30, 2015
    Publication date: April 7, 2016
    Inventors: Keith Leonard House, Benedict Yorke Johnson
  • Patent number: 9192632
    Abstract: Methods of producing stem cell conditioned media to treat mammalian injuries or insults. In at least one embodiment of a method of producing a stem cell conditioned media of the present disclosure, the method comprises the steps of culturing at least one stem cell in a first cell culture medium, replacing some or all of the first cell culture medium with a second cell culture medium and further culturing the at least one stem cell in the second cell culture medium, and collecting a quantity of the second cell culture medium after a culture duration, wherein the quantity of the second cell culture medium contains a cell culture byproduct effective to treat a mammalian insult or injury. In another embodiment, the step of culturing comprises culturing the at least one stem cell in EGM2MV.
    Type: Grant
    Filed: May 22, 2012
    Date of Patent: November 24, 2015
    Assignee: NeuroFx, Inc.
    Inventors: Keith Leonard March, Brian H. Johnstone, Yansheng Du
  • Publication number: 20150164953
    Abstract: The disclosure of the present application provides compositions and methods of treatment with stem cells. In at least one embodiment of a method for treating a patient with an insulin-related disorder, the method comprises the step of administering a cell-based composition to a patient with an insulin-related disorder to treat the insulin-related disorder, the cell-based composition comprising at least one mammalian stem cell and optionally at least one islet cell, the at least one mammalian stem cell capable of prolonging an effective life of the at least one islet cell.
    Type: Application
    Filed: December 9, 2014
    Publication date: June 18, 2015
    Inventors: Keith Leonard March, Carmella Evans Molina