Abstract: Methods for predicting the development of sepsis in a subject at risk for developing sepsis are provided. In one method, features in a biomarker profile of the subject are evaluated. The subject is likely to develop sepsis if these features satisfy a particular value set. Methods for predicting the development of a stage of sepsis in a subject at risk for developing a stage of sepsis are provided. In one method, a plurality of features in a biomarker profile of the subject is evaluated. The subject is likely to have the stage of sepsis if these feature values satisfy a particular value set. Methods of diagnosing sepsis in a subject are provided. In one such method, a plurality of features in a biomarker profile of the subject is evaluated. The subject is likely to develop sepsis when the plurality of features satisfies a particular value set.

Abstract: Methods for predicting the development of sepsis in a subject at risk for developing sepsis are provided. In one method, features in a biomarker profile of the subject are evaluated. The subject is likely to develop sepsis if these features satisfy a particular value set. Methods for predicting the development of a stage of sepsis in a subject at risk for developing a stage of sepsis are provided. In one method, a plurality of features in a biomarker profile of the subject is evaluated. The subject is likely to have the stage of sepsis if these feature values satisfy a particular value set. Methods of diagnosing sepsis in a subject are provided. In one such method, a plurality of features in a biomarker profile of the subject is evaluated. The subject is likely to develop sepsis when the plurality of features satisfies a particular value set.

Abstract: Methods for predicting the development of sepsis in a subject at risk for developing sepsis are provided. In one method, features in a biomarker profile of the subject are evaluated. The subject is likely to develop sepsis if these features satisfy a particular value set. Methods for predicting the development of a stage of sepsis in a subject at risk for developing a stage of sepsis are provided. In one method, a plurality of features in a biomarker profile of the subject is evaluated. The subject is likely to have the stage of sepsis if these feature values satisfy a particular value set. Methods of diagnosing sepsis in a subject are provided. In one such method, a plurality of features in a biomarker profile of the subject is evaluated. The subject is likely to develop sepsis when the plurality of features satisfies a particular value set.

Abstract: Systems and methods for inflating a tire are described herein. The systems and methods use a centrifugal force acting on a piston or the inertia of the piston to compress air to inflate the tire. Compressed air is ported into the tire, maintaining the pressure of the tire.

Abstract: Methods for predicting the development of sepsis in a subject at risk for developing sepsis are provided. In one method, features in a biomarker profile of the subject are evaluated. The subject is likely to develop sepsis if these features satisfy a particular value set. Methods for predicting the development of a stage of sepsis in a subject at risk for developing a stage of sepsis are provided. In one method, a plurality of features in a biomarker profile of the subject is evaluated. The subject is likely to have the stage of sepsis if these feature values satisfy a particular value set. Methods of diagnosing sepsis in a subject are provided. In one such method, a plurality of features in a biomarker profile of the subject is evaluated. The subject is likely to develop sepsis when the plurality of features satisfies a particular value set.

Abstract: Nucleic acids sequences that can be used for nucleic acid amplification, for example quantitative nucleic acid amplification, are provided herein.

Abstract: Methods for predicting the development of sepsis in a subject at risk for developing sepsis are provided. In one method, features in a biomarker profile of the subject are evaluated. The subject is likely to develop sepsis if these features satisfy a particular value set. Methods for predicting the development of a stage of sepsis in a subject at risk for developing a stage of sepsis are provided. In one method, a plurality of features in a biomarker profile of the subject is evaluated. The subject is likely to have the stage of sepsis if these feature values satisfy a particular value set. Methods of diagnosing sepsis in a subject are provided. In one such method, a plurality of features in a biomarker profile of the subject is evaluated. The subject is likely to develop sepsis when the plurality of features satisfies a particular value set.

Abstract: A method determines the position and/or radius of the limbus and/or the position and/or radius of the pupil of a patient eye. In the method, an image of the patient eye is obtained and a plurality of different ring-shaped comparison objects having respective radii and respective centers are provided. The image is correlated with the plurality of comparison objects to yield a local best match between the image and the comparison objects when there is a coincidence of one of the ring-shaped comparison objects and a ring-shaped jump in brightness in the image having the same radius and the same center. The comparison objects having a local best match with the image are determined. Thereafter, the position of the center of the comparison object having a local best match with the image is selected as the position of the center of the limbus and/or the position of the center of the pupil.

Abstract: Methods for predicting the development of sepsis in a subject at risk for developing sepsis are provided. In one method, features in a biomarker profile of the subject are evaluated. The subject is likely to develop sepsis if these features satisfy a particular value set. Methods for predicting the development of a stage of sepsis in a subject at risk for developing a stage of sepsis are provided. In one method, a plurality of features in a biomarker profile of the subject is evaluated. The subject is likely to have the stage of sepsis if these feature values satisfy a particular value set. Methods of diagnosing sepsis in a subject are provided. In one such method, a plurality of features in a biomarker profile of the subject is evaluated. The subject is likely to develop sepsis when the plurality of features satisfies a particular value set.

Abstract: A method determines the position and/or radius of the limbus and/or the position and/or radius of the pupil of a patient eye. In the method, an image of the patient eye is obtained and a plurality of different ring-shaped comparison objects having respective radii and respective centers are provided. The image is correlated with the plurality of comparison objects to yield a local best match between the image and the comparison objects when there is a coincidence of one of the ring-shaped comparison objects and a ring-shaped jump in brightness in the image having the same radius and the same center. The comparison objects having a local best match with the image are determined. Thereafter, the position of the center of the comparison object having a local best match with the image is selected as the position of the center of the limbus and/or the position of the center of the pupil.

Abstract: A method determines the position and/or radius of the limbus and/or the position and/or radius of the pupil of a patient eye. In the method, an image of the patient eye is obtained and a plurality of different ring-shaped comparison objects having respective radii and respective centers are provided. The image is correlated with the plurality of comparison objects to yield a local best match between the image and the comparison objects when there is a coincidence of one of the ring-shaped comparison objects and a ring-shaped jump in brightness in the image having the same radius and the same center. The comparison objects having a local best match with the image are determined. Thereafter, the position of the center of the comparison object having a local best match with the image is selected as the position of the center of the limbus and/or the position of the center of the pupil.

Abstract: The invention employs an unlabeled signal primer comprising a 5? adapter sequence for detection of variations in nucleic acid target sequences. The detection system further comprises a reporter probe, the 3? end of which hybridizes to the complement of the 5? adapter sequence of the signal primer to produce a 5? overhang. Polymerase is used to fill in the overhang and synthesize the complement of the 5? overhang of the reporter probe. Synthesis of the reporter probe complement is detected, either directly or indirectly, as an indication of the presence of the target.

Abstract: The invention relates to an eye surgery microscopy system (1) having an imaging optic (14, 11) for the generation of the image of an object plane (15) and having an electronic image sensor (22), which detects the image of the object plane (15) and is connected to a computer unit (5) for the computation of the position of the center of a circular structure (44) of a patient eye (16). The computer unit (5) is designed for the computation of the position of a patient eye (16) outside of the center (52) of the circular structure (44) and provided with at least one marking (46, 48). The computer unit (5) determines the position of the at least one marking (46, 48) with reference to the computed center (52) by means of image processing via correlation with a comparison information, and an angular position of the at least one marking (46, 48) with reference to the computed center (52) by means of image processing.

Abstract: A high-sensitivity, low-background immuno-amplification assay is provided, which offers a streamlined workflow suitable for high-throughput assays of clinically relevant samples, such as blood and other bodily fluids. The assay comprises the use of two proximity members that each comprise an analyte-specific binding component conjugated to an oligonucleotide. Binding an analyte brings the oligonucleotide moieties of the proximity members in sufficiently close contact that the oligonucleotides form an amplicon. The presence of the analyte then is detected through amplification of the amplicon and detection of the amplified nucleic acids. The sensitivity of the assay of the present invention is improved by preventing spurious or non-specific amplicon formation by proximity members that are not complexed with an analyte.

Abstract: Methods for predicting the development of sepsis in a subject at risk for developing sepsis are provided. In one method, features in a biomarker profile of the subject are evaluated. The subject is likely to develop sepsis if these features satisfy a particular value set. Methods for predicting the development of a stage of sepsis in a subject at risk for developing a stage of sepsis are provided. In one method, a plurality of features in a biomarker profile of the subject is evaluated. The subject is likely to have the stage of sepsis if these feature values satisfy a particular value set. Methods of diagnosing sepsis in a subject are provided. In one such method, a plurality of features in a biomarker profile of the subject is evaluated. The subject is likely to develop sepsis when the plurality of features satisfies a particular value set.

Abstract: A high-sensitivity, low-background immuno-amplification assay is provided, which offers a streamlined workflow suitable for high-throughput assays of clinically relevant samples, such as blood and other bodily fluids. The assay comprises the use of two proximity members that each comprise an analyte-specific binding component conjugated to an oligonucleotide. Binding an analyte brings the oligonucleotide moieties of the proximity members in sufficiently close contact that the oligonucleotides form an amplicon. The presence of the analyte then is detected through amplification of the amplicon and detection of the amplified nucleic acids. The sensitivity of the assay of the present invention is improved by preventing spurious or non-specific amplicon formation by proximity members that are not complexed with an analyte.

Abstract: A method determines the position and/or radius of the limbus and/or the position and/or radius of the pupil of a patient eye. In the method, an image of the patient eye is obtained and a plurality of different ring-shaped comparison objects having respective radii and respective centers are provided. The image is correlated with the plurality of comparison objects to yield a local best match between the image and the comparison objects when there is a coincidence of one of the ring-shaped comparison objects and a ring-shaped jump in brightness in the image having the same radius and the same center. The comparison objects having a local best match with the image are determined. Thereafter, the position of the center of the comparison object having a local best match with the image is selected as the position of the center of the limbus and/or the position of the center of the pupil.

Abstract: The invention relates to an eye surgery microscopy system (1) having an imaging optic (14, 11) for the generation of the image of an object plane (15) and having an electronic image sensor (18), which detects the image of the object plane (15) and is connected to a computer unit (5) for the computation of the position of the center of a circular structure (44) of a patient eye (16). The computer unit (5) is designed for the computation of the position of a patient eye (16) outside of the center (52) of the circular structure (44) and provided with at least one marking (46, 48). The computer unit (5) determines the position of the at least one marking (46, 48) with reference to the computed center (52) by means of image processing via correlation with a comparison information, and an angular position of the at least one marking (46, 48) with reference to the computed center (52) by means of image processing.

Abstract: Methods for predicting the development of sepsis in a subject at risk for developing sepsis are provided. In one method, features in a biomarker profile of the subject are evaluated. The subject is likely to develop sepsis if these features satisfy a particular value set. Methods for predicting the development of a stage of sepsis in a subject at risk for developing a stage of sepsis are provided. In one method, a plurality of features in a biomarker profile of the subject is evaluated. The subject is likely to have the stage of sepsis if these feature values satisfy a particular value set. Methods of diagnosing sepsis in a subject are provided. In one such method, a plurality of features in a biomarker profile of the subject is evaluated. The subject is likely to develop sepsis when the plurality of features satisfies a particular value set.

Abstract: The invention employs an unlabeled signal primer comprising a 5? adapter sequence for detection of variations in nucleic acid target sequences. The detection system further comprises a reporter probe, the 3? end of which hybridizes to the complement of the 5? adapter sequence of the signal primer to produce a 5? overhang. Polymerase is used to fill in the overhang and synthesize the complement of the 5? overhang of the reporter probe. Synthesis of the reporter probe complement is detected, either directly or indirectly, as an indication of the presence of the target.