Patents by Inventor Keith V. Wood

Keith V. Wood has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • NOVEL LUCIFERASE SEQUENCES UTILIZING INFRARED-EMITTING SUBSTRATES TO PRODUCE ENHANCED LUMINESCENCE
    Publication number: 20170314061
    Abstract: Provided herein are isolated polynucleotide encoding modified click beetle luciferase polypeptides that have enhanced luminescence and longer wavelength near-infrared signals. The disclosure also relates to near-infrared bioluminescence systems that include said modified click beetle luciferase polypeptides and novel luciferin derivatives, as well as methods of using said modified click beetle luciferase polypeptides and bioluminescence systems.
    Type: Application
    Filed: July 10, 2017
    Publication date: November 2, 2017
    Inventors: Lance P. Encell, Mary P. Hall, Keith V. Wood, Monika G. Wood
  • Synthetic luciferases with enhanced light output
    Patent number: 9777311
    Abstract: A polynucleotide encoding a modified luciferase polypeptide. The modified luciferase polypeptide has at least 60% amino acid sequence identity to a wild-type Oplophorus luciferase and includes at least one amino acid substitution at a position corresponding to an amino acid in a wild-type Oplophorus luciferase of SEQ ID NO:1. The modified luciferase polypeptide has at least one of enhanced luminescence, enhanced signal stability, and enhanced protein stability relative to the wild-type Oplophorus luciferase.
    Type: Grant
    Filed: October 14, 2013
    Date of Patent: October 3, 2017
    Assignee: PROMEGA CORPORATION
    Inventors: Lance P. Encell, Mary Hall, Paul Otto, Gediminas Vidugiris, Keith V. Wood, Monika G. Wood, Kristopher Zimmerman
  • COELENTERAZINE ANALOGUES
    Publication number: 20170233789
    Abstract: Described are coelenterazine analogues, methods for making the analogues, kits comprising the analogues, and methods of using the compounds for the detection of luminescence in luciferase-based assays.
    Type: Application
    Filed: February 14, 2017
    Publication date: August 17, 2017
    Inventors: Anton Shakhmin, Thomas Kirkland, Joel Walker, Thomas Machleidt, Mary Hall, Keith V. Wood
  • Luciferase sequences utilizing infrared-emitting substrates to produce enhanced luminescence
    Patent number: 9732373
    Abstract: Provided herein are isolated polynucleotide encoding modified click beetle luciferase polypeptides that have enhanced luminescence and longer wavelength near-infrared signals. The disclosure also relates to near-infrared bioluminescence systems that include said modified click beetle luciferase polypeptides and novel luciferin derivatives, as well as methods of using said modified click beetle luciferase polypeptides and bioluminescence systems.
    Type: Grant
    Filed: September 11, 2015
    Date of Patent: August 15, 2017
    Assignee: Promega Corporation
    Inventors: Lance P. Encell, Mary P. Hall, Keith V. Wood, Monika G. Wood
  • LUMINESCENCE-BASED METHODS AND PROBES FOR MEASURING CYTOCHROME P450 ACTIVITY
    Publication number: 20170191985
    Abstract: The invention provides compounds, compositions, methods, substrates, and kits useful for analyzing the metabolic activity in cells, tissue, and animals and for screening test compounds for their effect on cytochrome P450 activity. In particular, a one-step and two-step methods using luminogenic molecules, e.g. luciferins or coelenterazines, that are cytochrome P450 substrates and that are also bioluminescent enzyme, e.g., luciferase, pro-substrates are provided. The present method further provides a method for stabilizing and prolonging the luminescent signal in a luciferase-based assay using luciferase stabilizing agents such as reversible lucifetrase inhibitors.
    Type: Application
    Filed: January 18, 2017
    Publication date: July 6, 2017
    Inventors: James J. Cali, Dieter Klaubert, William Daily, Samuel Kin Sang Ho, Susan Frackman, Erika Hawkins, Keith V. Wood
  • Compositions and methods for stabilizing luminogenic substrates
    Patent number: 9676997
    Abstract: Disclosed herein are compositions and methods for stabilizing a luminogenic substrate such as coelenterazine or a functional analog thereof. The functional analog may be furimazine. The composition may include the luminogenic substrate, a thionucleoside, and an organic solvent, in which the thionucleoside is present in an amount effective to stabilize the luminogenic composition against decomposition. The method provided herein stabilizes the luminogenic substrate against decomposition by contacting the luminogenic substrate with an effective amount of the thionucleoside in the presence of the organic solvent. Also provided herein is a kit containing the composition.
    Type: Grant
    Filed: May 26, 2015
    Date of Patent: June 13, 2017
    Assignee: PROMEGA CORPORATION
    Inventors: Thomas Kirkland, Keith V. Wood, Mary Hall, Marie Schwinn
  • POLYNUCLEOTIDES ENCODING MUTANT HYDROLASE PROTEINS WITH ENHANCED KINETICS AND FUNCTIONAL EXPRESSION
    Publication number: 20170137793
    Abstract: The invention provides a mutant hydrolase protein with enhanced kinetics and functional expression, as well as polynucleotides encoding the mutant proteins and methods of using the polynucleotides and mutant proteins.
    Type: Application
    Filed: January 24, 2017
    Publication date: May 18, 2017
    Inventors: Aldis Darzins, Lance P. Encell, Rachel Friedman Ohana, Paul Otto, Michael R. Slater, Gediminas Vidugiris, Keith V. Wood, Monika G. Wood, Kate Qin Zhao, Kris Zimmerman
  • Luciferase-based assays
    Patent number: 9631225
    Abstract: A method and kit are provided for enhancing the tolerance of an assay reagent to compounds in an assay sample, the assay reagent including a luciferase enzyme. The method includes contacting the luciferase with a tolerance enhancement agent in an amount sufficient to substantially protect luciferase enzyme activity from interference of the compound and minimize interference by at least about 10% relative to an assay not having tolerance enhancement agent.
    Type: Grant
    Filed: October 14, 2014
    Date of Patent: April 25, 2017
    Assignee: PROMEGA CORPORATION
    Inventors: Erika Hawkins, James J. Cali, Samuel Kin Sang Ho, Martha A. O'Brien, Richard Somberg, Robert F. Bulleit, Keith V. Wood
  • COVALENT TETHERING OF FUNCTIONAL GROUPS TO PROTEINS AND SUBSTRATES THEREFOR
    Publication number: 20170097356
    Abstract: A mutant hydrolase optionally fused to a protein of interest is provided. The mutant hydrolase is capable of forming a bond with a substrate for the corresponding nonmutant (wild-type) hydrolase which is more stable than the bond formed between the wild-type hydrolase and the substrate and has at least two amino acid substitutions relative to the wild-type hydrolase. Substrates for hydrolases comprising one or more functional groups are also provided, as well as methods of using the mutant hydrolase and the substrates of the invention. Also provided is a fusion protein capable of forming a stable bond with a substrate and cells which express the fusion protein.
    Type: Application
    Filed: August 16, 2016
    Publication date: April 6, 2017
    Inventors: Aldis Darzins, Lance P. Encell, Dieter Klaubert, Georgyi V. Los, Mark McDougall, Keith V. Wood, Monika G. Wood, Chad Zimprich
  • COVALENT TETHERING OF FUNCTIONAL GROUPS TO PROTEINS
    Publication number: 20170088877
    Abstract: A mutant hydrolase optionally fused to a protein of interest is provided. The mutant hydrolase is capable of forming a bond with a substrate for the corresponding nonmutant (wild-type) hydrolase which is more stable than the bond formed between the wild-type hydrolase and the substrate. Substrates for hydrolases comprising one or more functional groups are also provided, as well as methods of using the mutant hydrolase and the substrates of the invention. Also provided is a fusion protein capable of forming a stable bond with a substrate and cells which express the fusion protein.
    Type: Application
    Filed: December 12, 2016
    Publication date: March 30, 2017
    Inventors: Keith V. Wood, Georgyi V. Los, Robert F. Bulleit, Dieter Klaubert, Mark McDougall, Chad Zimprich
  • Polynucleotides encoding mutant hydrolase proteins with enhanced kinetics and functional expression
    Patent number: 9593316
    Abstract: The invention provides a mutant hydrolase protein with enhanced kinetics and functional expression, as well as polynucleotides encoding the mutant proteins and methods of using the polynucleotides and mutant proteins.
    Type: Grant
    Filed: May 22, 2014
    Date of Patent: March 14, 2017
    Assignee: Promega Corporation
    Inventors: Aldis Darzins, Lance P. Encell, Rachel Friedman Ohana, Paul Otto, Michael R. Slater, Gediminas Vidugiris, Keith V. Wood, Monika G. Wood, Kate Qin Zhao, Kris Zimmerman
  • Luminescence-based methods and probes for measuring cytochrome P450 activity
    Patent number: 9574223
    Abstract: The invention provides compounds, compositions, methods, substrates, and kits useful for analyzing the metabolic activity in cells, tissue, and animals and for screening test compounds for their effect on cytochrome P450 activity. In particular, a one-step and two-step methods using luminogenic molecules, e.g. luciferins or coelenterazines, that are cytochrome P450 substrates and that are also bioluminescent enzyme, e.g., luciferase, pro-substrates are provided. The present method further provides a method for stabilizing and prolonging the luminescent signal in a luciferase-based assay using luciferase stabilizing agents such as reversible luciferase inhibitors.
    Type: Grant
    Filed: June 30, 2014
    Date of Patent: February 21, 2017
    Assignee: PROMEGA CORPORATION
    Inventors: James J. Cali, Dieter Klaubert, William Daily, Samuel Kin Sang Ho, Susan Frackman, Erika Hawkins, Keith V. Wood
  • Covalent tethering of functional groups to proteins
    Patent number: 9540402
    Abstract: A mutant hydrolase optionally fused to a protein of interest is provided. The mutant hydrolase is capable of forming a bond with a substrate for the corresponding nonmutant (wild-type) hydrolase which is more stable than the bond formed between the wild-type hydrolase and the substrate. Substrates for hydrolases comprising one or more functional groups are also provided, as well as • methods of using the mutant hydrolase and the substrates of the invention. Also provided is a fusion protein capable of forming a stable bond with a substrate and cells which express the fusion protein.
    Type: Grant
    Filed: December 29, 2014
    Date of Patent: January 10, 2017
    Assignee: PROMEGA CORPORATION
    Inventors: Keith V. Wood, Georgyi V. Los, Robert F. Bulleit, Dieter Klaubert, Mark McDougall, Chad Zimprich
  • CELL-PERMEABLE, CELL-COMPATIBLE, AND CLEAVABLE LINKERS FOR COVALENT TETHERING OF FUNCTIONAL ELEMENTS
    Publication number: 20160355523
    Abstract: Provided herein are cell-permeable, cell-compatible, and chemoselectively-cleavable linkers for tethering (e.g., covalently) functional elements (e.g., a cellular interaction element and a capture element), and methods of use (e.g., intracellular capture and extracellular release of cellular targets) therewith.
    Type: Application
    Filed: June 3, 2016
    Publication date: December 8, 2016
    Inventors: Sergiy Levin, Rachel Friedman Ohana, Thomas Kirkland, Keith V. Wood
  • PERMUTED AND NONPERMUTED LUCIFERASE BIOSENSORS
    Publication number: 20160340654
    Abstract: A modified luciferase protein which is a sensor for molecules including cAMP, cGMP, calcium, chelators thereof, kinases, or phosphatases is provided. Also provided is a circularly permuted anthozoan luciferase protein and a decapod crustacean luciferase protein, optionally containing one or more heterologous amino acid sequences, at least one of which directly or indirectly interacts with a molecule of interest. Further provided is a modified anthozoan luciferase protein and a decapod crustacean luciferase protein containing an insertion of one or more heterologous amino acid sequences, at least one of which directly or indirectly interacts with a molecule of interest.
    Type: Application
    Filed: May 31, 2016
    Publication date: November 24, 2016
    Inventors: Brock Binkowski, Frank Fan, Susan Wigdal, Keith V. Wood, Monika G. Wood
  • STABILIZED FORMULATION FOR LUMINESCENT DETECTION OF LUCIFERASE AND NUCLEOSIDE PHOSPHATES
    Publication number: 20160326570
    Abstract: Methods, kits and compositions containing a mixture of D-luciferin and L-luciferin for light generation with luciferase are disclosed that have improved stability when stored over time. The mixture of D-luciferin and L-luciferin can be used to detect the presence or amount of ATP or of luciferase in a sample.
    Type: Application
    Filed: July 20, 2016
    Publication date: November 10, 2016
    Inventors: Michael P. Valley, James J. Cali, Brock Binkowski, Christopher Todd Eggers, Keith V. Wood
  • Stabilized formulation for luminescent detection of luciferase and nucleoside phosphates
    Patent number: 9487814
    Abstract: Methods, kits and compositions containing a mixture of D-luciferin and L-luciferin for light generation with luciferase are disclosed that have improved stability when stored over time. The mixture of D-luciferin and L-luciferin can be used to detect the presence or amount of ATP or of luciferase in a sample.
    Type: Grant
    Filed: February 21, 2014
    Date of Patent: November 8, 2016
    Assignee: PROMEGA CORPORATION
    Inventors: Michael P. Valley, James J. Cali, Brock Binkowski, Christopher Todd Eggers, Keith V. Wood
  • Covalent tethering of functional groups to proteins and substrates therefor
    Patent number: 9416353
    Abstract: A mutant hydrolase optionally fused to a protein of interest is provided. The mutant hydrolase is capable of forming a bond with a substrate for the corresponding nonmutant (wild-type) hydrolase which is more stable than the bond formed between the wild-type hydrolase and the substrate and has at least two amino acid substitutions relative to the wild-type hydrolase. Substrates for hydrolases comprising one or more functional groups are also provided, as well as methods of using the mutant hydrolase and the substrates of the invention. Also provided is a fusion protein capable of forming a stable bond with a substrate and cells which express the fusion protein.
    Type: Grant
    Filed: June 2, 2014
    Date of Patent: August 16, 2016
    Assignee: PROMEGA CORPORATION
    Inventors: Aldis Darzins, Lance P. Encell, Dieter Klaubert, Georgyi V. Los, Mark McDougall, Keith V. Wood, Monika G. Wood, Chad Zimprich
  • LUMINOGENIC AND FLUOROGENIC COMPOUNDS AND METHODS TO DETECT MOLECULES OR CONDITIONS
    Publication number: 20160176830
    Abstract: A method to detect the presence of amount of at least one molecule in a sample which employs a derivative of luciferin or a derivative of a fluorophore is provided. Compounds and compositions for carrying out the methods of the invention are also provided.
    Type: Application
    Filed: November 25, 2014
    Publication date: June 23, 2016
    Inventors: James J. Cali, William Daily, Erika Hawkins, Dieter Klaubert, Jianquan Liu, Poncho Meisenheimer, Michael Scurria, John W. Shultz, James Unch, Michael P. Valley, Keith V. Wood, Wenhui Zhou
  • Vectors for directional cloning
    Patent number: 9371531
    Abstract: The invention provides vectors and methods for directional cloning.
    Type: Grant
    Filed: January 14, 2013
    Date of Patent: June 21, 2016
    Assignee: PROMEGA CORPORATION
    Inventors: Michael R. Slater, Ethan Edward Strauss, Keith V. Wood, James Robert Hartnett