Abstract: A modular bioreactor system is provided including a platform having a seat to receive a modular tissue chamber, and one or more platform interfacial members; and a releasable modular tissue chamber including a bottom surface, an open top, and sidewalls defining a well therein, the releasable modular tissue chamber configured to be received by the seat of the platform, the tissue chamber further including an interfacial member configured to releasably connect the modular tissue chamber to the platform, wherein a tissue chamber contains human bone marrow (engineered using iPS cell derived osteoblasts, osteoclasts, endothelial cells, supporting mesenchymal cells, and hematopoietic cells in bone matrix) infused by primary leukemia cells

Abstract: A medical apparatus having an elongate tubular body with a proximal end, an opening at the proximal end, and a distal end defining an interior space therebetween and the distalmost end of the elongate tubular body is configured to matingly engage a filter assembly; a filter assembly configured to matingly engage the distal portion of the elongate tubular body and to provide a seal with the interior space thereof, the filter assembly comprising a filter membrane and a border that is sectionable for mounting on a slide for analysis on a microscope; and a base member configured to releasably engage the distalmost end of the elongate tubular body and to receive the filter assembly therein.

Abstract: An alternating vacuum and pressure system for decellularizing a bone matrix includes piping having a first end open to atmospheric pressure and a second end open to atmospheric pressure, and a subset of piping that forms a loop, a chamber in fluid, at least one heating element configured to heat the piping, a pump, and a plurality of valves. The plurality of valves can be selectively opened or closed to form one of a plurality of configurations, including a pressure configuration in which operation of the pump in a first direction causes a fluid within the piping to travel toward the chamber such that pressure is applied to the chamber and a vacuum configuration in which operation of the pump in a second direction causes the fluid within the pipe to travel away from the chamber such that a vacuum is created on the chamber.

Abstract: A system for seeding therapeutic active ingredients (TAIs) onto a porous scaffold includes a first chamber for accommodating the scaffold, a TAI storage device for storing TAIs, at least one second chamber for storing TAI media, and a gas inlet for receiving gas from a compressed source. A flow circuit is coupled to the first chamber, the TAI storage device, the second chamber and the gas inlet for delivering the TAIs, the TAI media and the gas to the scaffold. A pump pumps at least one of the TAIs, the TAI media and the gas in the flow circuit. The system also includes a processor that regulates the delivery of the TAIs, the TAI media and the gas to the scaffold via a plurality of valves.

Abstract: A medical apparatus having an elongate tubular body with a proximal end, an opening at the proximal end, and a distal end defining an interior space therebetween and the distalmost end of the elongate tubular body is configured to matingly engage a filter assembly; a filter assembly configured to matingly engage the distal portion of the elongate tubular body and to provide a seal with the interior space thereof, the filter assembly comprising a filter membrane and a border that is sectionable for mounting on a slide for analysis on a microscope; and a base member configured to releasably engage the distalmost end of the elongate tubular body and to receive the filter assembly therein.

Abstract: A method of detecting or diagnosing squamous cancers in a sample from a subject, including isolating extracellular vesicles from the sample, wherein the extracellular vesicles are cancer specific.

Abstract: Disclosed are systems and methods for culturing systemic bioengineered tumor models, including osteosarcoma cells or breast adenocarcinoma cells derived from patients. The model includes the tumor cells on host tissues such as bone, liver, lung, and heart tissue, wherein the bone, liver, lung, and heart tissue are separated by endothelial barriers. Beneath the endothelial barriers the tissues are connected via circulation containing secreted factors and cells (e.g., tumor/immune cells).

Abstract: Drilling devices, systems, kits and methods for drilling cartilage defects are disclosed. A system includes a handle device having an adjustable shaft collar rotatable to adjust a drill set distance between the adjustable shaft collar and the handle. The handle device can include a distal drill guide extending from the handle at an end opposite the adjustable shaft collar. The system includes a drill collar attachable to a drill bit and configured to remain stationary along a length of the drill bit once attached. The system includes a rotatable disk positionable between the adjustable shaft collar and the drill collar. The rotatable disk can include a central hole sized to accept the drill bit.

Abstract: Embodiments described herein relate generally to devices, apparatuses, and systems with embedded electrodes for rowing, maintaining, and/or using 3D tissues in vitro. The devices, apparatuses, and systems described herein can provide scalable, automated tissue stimulation.

Abstract: An anatomically-shaped, human bone graft may be cultivated ex vivo using a bioreactor capable of perfusing large complex porous scaffolds. Scaffolds derived from image-based modeling of a target are seeded with human mesenchymal stem cells and cultivated. A bioreactor configured to house complex three-dimensional scaffold geometries provides controlled flow for perfusion of the cells. Dense uniform cellular growth can be attained throughout the entire scaffold as a result of the medium perfusion. In an embodiment, the bioreactor has a mold into which perfusion medium is pumped under pressure and multiple ports through which the medium exits the mold.

Abstract: A medical apparatus having an elongate tubular body with a proximal end, an opening at the proximal end, and a distal end defining an interior space therebetween and the distalmost end of the elongate tubular body is configured to matingly engage a filter assembly, a filter assembly configured to matingly engage the distal portion of the elongate tubular body and to provide a seal with the interior space thereof, the filter assembly comprising a filter membrane and a border that is sectionable for mounting on a slide for analysis on a microscope; and a base member configured to releasably engage the distalmost end of the elongate tubular body and to receive the filter assembly therein.

Abstract: An anatomically-shaped, human bone graft may be cultivated ex vivo using a bioreactor capable of perfusing large complex porous scaffolds. Scaffolds derived from image-based modeling of a target are seeded with human mesenchymal stem cells and cultivated. A bioreactor configured to house complex three-dimensional scaffold geometries provides controlled flow for perfusion of the cells. Dense uniform cellular growth can be attained throughout the entire scaffold as a result of the medium perfusion. In an embodiment, the bioreactor has a mold into which perfusion medium is pumped under pressure and multiple ports through which the medium exits the mold.

Abstract: A cardiac organoid containing 3-D matter of adult human heart tissue.

Abstract: A medical apparatus and method of preparing one or more cell blocks. The medical apparatus comprises at least one elongate tubular body having a proximal end and a distal end and filter assembly comprising a filter membrane and a border configured to engage the tubular body. The filter membrane and the border are each formed from material that is sectionable.

Abstract: A cardiac organoid containing 3-D matter of adult human heart tissue.

Abstract: A bioreactor is provided that permits engineering of multiple different tissues. The bioreactor has a series of flow paths that permit application of tissue-specific media while simultaneously innervating the various different tissues with a common media. The flow paths for the various medias are designed to prevent mixing of the various media as they simultaneously innervate the tissue.

Abstract: A medical apparatus is provided including an elongated body and defining a loading chamber between a proximal end and a distal end thereof for storing a liquid therein, the elongated body defining an outlet at the distal end thereof; a cell block filter assembly including a cell block body and defining a well portion matingly engageable with the distal end of the elongated body for receiving fluid from the outlet of the elongated body and defining a well opening on a bottom thereof; a filter membrane disposed across the well opening on the bottom of the well portion; and a cover member positionable over the well portion wherein the entire cell block filter assembly is sliceable into slices having a thickness suitable for mounting on a laboratory slide.

Abstract: An integrated modular microphysiological system is provided which includes a two or more chambers and a vascular network which includes at least one channel. The chamber can be configured for culturing a tissue and includes a layer of endothelial cells which forms an endothelial barrier within the well. The endothelial barrier can be in fluid contact with at least one of the at least one channels in the vascular network. The endothelial barrier can also be in fluid contact with a fluid in the chamber.

Abstract: A system is provided for evaluating the function of the neuromuscular junction (NMJ) of a subject, which includes a platform including first and second culture chambers separated by a channel; the platform supporting a microtissue culture including: human skeletal myoblasts derived from the subject in the first chamber; a neurosphere derived from the subject, expressing an optogenetic protein in the second chamber, and a hydrogel in the channel to allow axonal sprouting and growth between the myoblasts and neurosphere. A light source is provided for optical stimulation pulses applied to the microtissue culture for activation of the optogenetic protein; and image recordation device for capturing images of the culture in response to the optical stimulation.

Abstract: A system for seeding therapeutic active ingredients (TAIs) onto a porous scaffold includes a first chamber for accommodating the scaffold, a TAI storage device for storing TAIs, at least one second chamber for storing TAI media, and a gas inlet for receiving gas from a compressed source. A flow circuit is coupled to the first chamber, the TAI storage device, the second chamber and the gas inlet for delivering the TAIs, the TAI media and the gas to the scaffold. A pump pumps at least one of the TAIs, the TAI media and the gas in the flow circuit. The system also includes a processor that regulates the delivery of the TAIs, the TAI media and the gas to the scaffold via a plurality of valves.