Abstract: Methods of administering lipid nanoparticle compositions including poly(ethyloxazoline)-lipid conjugates (PEOZ-lipid LNPs) without triggering a PEOZ-associated immune response. In some aspects, the PEOZ-lipid LNPs trigger a protective response based on the encapsulated payload but do not trigger an IgM or IgG response. also provides an absent or. In other aspects, the PEOZ-lipid LNPs trigger a protective response based on the encapsulated payload but trigger an IgM and IgG response that is markedly reduced (as compared to a comparable PEG-lipid currently used in LNP vaccine delivery systems). PEOZ-DMA LNPs incorporating payloads such as oligonucleotides payloads mRNA, DNA, and siRNA for delivery into living cells is also contemplated.

Abstract: Poly(oxazoline) conjugates with pendant cationic groups (cationic POZ) and lipid nanoparticles (LNPs) including cationic POZ used to facilitate delivery of an encapsulated payload. LNPs and polyplexes including cationic POZ and a nucleic acid payload such as, but not limited to, mRNA or modified mRNA are disclosed. Such LNPs have no immunogenicity or reduced immunogenicity as compared to a corresponding LNP containing an ionizable lipid.

Abstract: A method is disclosed for the Agrobacterium-mediated germline transformation of soybean, comprising infecting split soybean seeds, with a portion of the embryonic axis, with Agrobacterium tumefaciens containing a transgene. The method can further comprise regenerating the explants produced from the transformation of the split soybean seeds comprising a portion of embryonic axis in vitro on selection medium.

Abstract: This invention pertains in part to the development of a vaccine for poultry against necrotic enteritis (NE). The vaccine utilizes a protective antigen that is a mutated, full-length, non-toxic Clostridium perfringens (Cp) ?-toxin protein (Mcpa). Utility of this vaccine was demonstrated by reduction of lesion severity in NE challenge trails, for example. Also disclosed herein are novel approaches for producing this vaccine in significant quantities. One exemplified approach involves producing NE vaccine (mutated alpha toxin) in bacterial expression systems, preferably utilizing the Pseudomonas fluorescens system, for commercial use in controlling NE in the poultry industry. The subject vaccines can be administered preferably to chickens in several different ways. A novel, Type VI alpha toxin from chicken isolates of Cp is also disclosed.

Abstract: The present invention relates to nucleic acid and amino acid sequences that confer herbicide resistance in plants, as well as herbicide resistance in plants, plant seeds, plant tissues and plant cells comprising such sequences. In a preferred embodiment, the sequences of the present invention confer a tolerant phenotype in plants in response to a chronic and/or acute inhibitin dose of auxinic herbicides. The present invention also provides homologous sequences with a high degree of functional similarity.

Abstract: Regulatory seqeunces derived from the maize per5 gene have utility in plant biotechnology.

Abstract: Regulatory sequences derived from the maize per5 gene have utility in plant biotechnology.

Abstract: Regulatory sequences derived from a maize root preferential cationic peroxidase gene (Per5), including the promoter, introns, and the 3′ untranslated region, are useful to control expression of recombinant genes in plants.