Abstract: Disclosed herein are methods of detecting HIV-1 nucleic acids in a sample (such as from a sample containing or suspected to contain HIV-1 nucleic acid). In some examples, the methods include loop-mediated isothermal amplification (LAMP) or reverse transcription-LAMP (RT-LAMP). In some examples, the methods include contacting a sample with one or more sets of LAMP primers specific for HIV-1 (such as LAMP primers specific for an HIV-1 integrase nucleic acid or LAMP primers specific for an HIV-1 reverse transcriptase nucleic acid) under conditions sufficient to produce an amplification product and detecting the amplification product. Sets of LAMP primers for detection of HIV-1 integrase nucleic acids (such as SEQ ID NOs: 8-14 or 8-27) and HIV-1 reverse transcriptase nucleic acids (such as SEQ ID NOs: 1-7) are provided herein.

Abstract: Disclosed herein are methods of detecting HIV-1 nucleic acids in a sample (such as from a sample containing or suspected to contain HIV-1 nucleic acid). In some examples, the methods include loop-mediated isothermal amplification (LAMP) or reverse transcription-LAMP (RT-LAMP). In some examples, the methods include contacting a sample with one or more sets of LAMP primers specific for HIV-1 (such as LAMP primers specific for an HIV-1 integrase nucleic acid or LAMP primers specific for an HIV-1 reverse transcriptase nucleic acid) under conditions sufficient to produce an amplification product and detecting the amplification product. Sets of LAMP primers for detection of HIV-1 integrase nucleic acids (such as SEQ ID NOs: 8-14 or 8-27) and HIV-1 reverse transcriptase nucleic acids (such as SEQ ID NOs: 1-7) are provided herein.

Abstract: Disclosed herein are methods of detecting HIV-2 nucleic acids in a sample (such as from a sample infected with or suspected to be infected with HIV-2). In some examples, the methods include LAMP or RT-LAMP, while in other examples, the methods include hybridization of a probe to an HIV-2 nucleic acid, including, but not limited to real-time PCR. Sets of LAMP primers for detection of HIV-2 Group A and Group B nucleic acids are provided herein. Sets of probes and primers for real-time PCR detection of HIV-2 nucleic acids are also provided herein. Finally, primers for amplification of HIV-2 nucleic acids are provided. Also disclosed are isolated HIV-2 nucleic acids, vectors including the HIV-2 nucleic acids, and cells transformed with vectors including HIV-2 nucleic acids.

Abstract: Methods and compositions for detection of HIV nucleic acids in a sample, such as a biological sample obtained from a human subject, are provided according to embodiments of the present invention which include providing a reaction mixture including at least one LAMP, accelerated LAMP, RT-LAMP or RT-accelerated LAMP assay primer set specific for HIV-I or HIV-2 nucleic acids and the biological sample to be tested for presence of HIV-I and/or HIV-2 nucleic acids; incubating the reaction mixture under conditions suitable to produce a LAMP assay reaction product; and detecting the reaction product. Primers and primer sets for use in LAMP assays of HIV-I or HIV-2 nucleic acids are provided.

Abstract: Methods and compositions for detection of HIV nucleic acids in a sample, such as a biological sample obtained from a human subject, are provided according to embodiments of the present invention which include providing a reaction mixture including at least one LAMP, accelerated LAMP, RT-LAMP or RT-accelerated LAMP assay primer set specific for HIV-I or HIV-2 nucleic acids and the biological sample to be tested for presence of HIV-I and/or HIV-2 nucleic acids; incubating the reaction mixture under conditions suitable to produce a LAMP assay reaction product; and detecting the reaction product. Primers and primer sets for use in LAMP assays of HIV-I or HIV-2 nucleic acids are provided.