Abstract: Provided herein are compositions, methods and uses that relate to or result from providing separation media having at least one flocculant ligand covalently attached to a base surface or support, and the separation and/or purification of biological molecules using the separation media of the present disclosure. Certain embodiments provide separation media which under certain modes of operation, enhance the separation of the molecule of interest from impurities.

Abstract: Provided herein are compositions, methods and uses that relate to or result from providing separation media having at least one flocculant ligand covalently attached to a base surface or support, and the separation and/or purification of biological molecules using the separation media of the present disclosure. Certain embodiments provide separation media which under certain modes of operation, enhance the separation of the molecule of interest from impurities.

Abstract: The present invention includes a novel method and system for identification of microorganisms in samples that proteins and other biological material from non-microorganism sources (e.g., proteins of mammalian origin) that can interfere with identification of the microorganisms. The methods and systems described herein include use of a single-use chromatography medium to purify intact proteins prior to mass spectrometry analysis. The chromatography medium and the methods described herein can rapidly and efficiently remove of a substantial portion of interfering biological material (e.g., mammalian proteins) from a crude cell lysate while preserving high signal strength and removing enough of the interfering protein(s) to allow for identification of the microorganism(s) by mass spectrometry analysis.

Abstract: Provided herein are compositions, methods and uses that relate to or result from providing separation media having at least one flocculant ligand covalently attached to a base surface or support, and the separation and/or purification of biological molecules using the separation media of the present disclosure. Certain embodiments provide separation media which under certain modes of operation, enhance the separation of the molecule of interest from impurities.

Abstract: The present invention includes a novel method and system for identification of microorganisms in samples that proteins and other biological material from non-microorganism sources (e.g., proteins of mammalian origin) that can interfere with identification of the microorganisms. The methods and systems described herein include use of a single-use chromatography medium to purify intact proteins prior to mass spectrometry analysis. The chromatography medium and the methods described herein can rapidly and efficiently remove of a substantial portion of interfering biological material (e.g., mammalian proteins) from a crude cell lysate while preserving high signal strength and removing enough of the interfering protein(s) to allow for identification of the microorganism(s) by mass spectrometry analysis.

Abstract: Described are extraction columns and systems that include extraction columns. The extraction column includes a column body that has a reservoir portion, an extraction media portion, and a collar portion. The reservoir portion includes an inlet and a reservoir. The extraction media portion includes an elongated sleeve having an inner surface defining a cavity. The extraction media portion has an inlet end in fluid communication with the reservoir and an outlet end. An extraction medium is disposed in the cavity of the elongated sleeve. The collar portion extends axially in a common direction with the elongated sleeve. The collar portion has a terminal end which is spaced apart from the outlet end of the elongated sleeve and which extends axially at least to a plane defined by the outlet end of the elongated sleeve.

Abstract: Described are extraction columns and systems that include extraction columns. The extraction column includes a column body that has a reservoir portion, an extraction media portion, and a collar portion. The reservoir portion includes an inlet and a reservoir. The extraction media portion includes an elongated sleeve having an inner surface defining a cavity. The extraction media portion has an inlet end in fluid communication with the reservoir and an outlet end. An extraction medium is disposed in the cavity of the elongated sleeve. The collar portion extends axially in a common direction with the elongated sleeve. The collar portion has a terminal end which is spaced apart from the outlet end of the elongated sleeve and which extends axially at least to a plane defined by the outlet end of the elongated sleeve.