Abstract: The technology relates in part to methods and compositions for analyzing nucleic acid. In some aspects, the technology relates to methods and compositions for preparing a nucleic acid library from single-stranded nucleic acid fragments.

Abstract: A piston seal assembly for a gas turbine engine includes a seal composed of a nickel-based superalloy; a component in contact with the seal and defining a seal-counterface; and a coating on the seal at the seal-counterface, wherein the coating is a metal alloy binder phase and a hard particle phase distributed through the binder phase.

Abstract: A seal assembly for a gas turbine engine includes a seal composed of a nickel-based superalloy; a component in contact with the seal and defining a seal-counterface; and a coating on the seal at the seal-counterface, wherein the coating is a ternary oxide.

Abstract: Methods are provided that, for example, include (a) combining ssDNA containing a modified nucleotide (e.g., a ssDNA with a modified nucleotide proximate to its 5? end) with a DNA cleavage enzyme capable of cleaving the ssDNA at the modified nucleotide (e.g., to generate a first ssDNA fragment having a 3?OH and a second ssDNA fragment having the modified nucleotide); wherein the ratio of enzyme to DNA substrate is less than 1:1 molar ratio (m/m); and (b) cleaving at least 95% of the ssDNA at the modified nucleotide. In some embodiments, a method may comprise (a) combining (i) a ssDNA comprising a modified nucleotide (e.g., proximate to its 5? end) with (ii) a DNA cleavage enzyme capable of cleaving the ssDNA at the modified nucleotide (e.g., to generate (after cleavage) a first ssDNA fragment having a 3?OH and a second ssDNA fragment comprising the modified nucleotide) wherein the ratio of enzyme to DNA substrate is less than 1:1 molar ratio and cleaving at least 95% of the ssDNA at the modified nucleotide.

Abstract: The invention relates to a method and apparatus for evaluating reaction molecular byproducts of pyrotechnic reactions. A closed calorimetry bomb holds pyrotechnic material, which is detonated by a charge. The calorimetry bomb is vented directly into a gas chromatography machine, where gas phase molecules are separated based on their polarity. The separated molecules are then injected into a mass spectrometer and characterized by their mass fragmentation. The remaining residual solids within the bomb are extracted and injected into a liquid chromatography instrument where they are separated by their polarity. The separated molecules are then injected into a mass spectrometer and characterized by their mass fragmentation pattern. The method provides a complete picture of the reaction pathways and products to aid in regulatory compliance of incorporating energetic materials into real-world applications, particularly those in the family of PFAS containing compositions.

Abstract: Disclosed is a Bulk Acoustic Wave (BAW) assist filter structure with a BAW resonator stacked onto an integrated passive device (IPD). In exemplary aspects disclosed herein, the BAW filter structure includes a transducer with electrodes and a piezoelectric layer between the electrodes. The IPD is electrically coupled to the BAW resonator and provides a high frequency of operation. In such a configuration, the BAW assist filter structure has a low insertion loss and mitigates electrical length parasitic loss due to the close electrically proximity of the BAW resonator stacked onto the IPD. Further, the BAW assist filter structure is able to filter high frequencies and provides improved filter performance and greater flexibility in design of a filter transfer function.

Abstract: A method for generating assembly instructions may include receiving inputs from a user, such that the inputs may include an indication of components of a product package. The method may also include retrieving assembly attribute data for each component from a first storage component, such that the assembly attribute data includes information regarding compatibility properties between two or more of the plurality of components. The method may then involve retrieving model data for each component from a second storage component, such that the model data is representative of physical properties of a respective component. The method may also include generating the assembly instructions for assembling the components together based on the attribute data and the model data, such that the assembly instructions include visualizations representative of at least a portion of a process for assembling the plurality of components together. The assembly instructions are then presented via an electronic display.

Abstract: A method for regulating use of stored diluent in a polymerization process so as to maximize use of diluent recycled diluent recovered from the process, and so minimize use of stored diluent, is disclosed. Also disclosed is apparatus, particularly as an arrangement of controllers and valves for handling the flow of diluent through the process that can be used to implement the disclosed process.

Abstract: A kit for dispensing disposable stethoscope covers and other instrument covers is provided. The kit may include a cartridge and a dispenser for automatic touch-free dispensing of disposable stethoscope covers. The kit further includes a first spool having a roll of backing member with disposable stethoscope covers disposed thereon and a second spool for receiving the spent backing member after a disposable stethoscope cover has been removed, self-contained within the cartridge. The cartridge is inserted within the dispenser so that a user may insert a stethoscope head through a window of the dispenser to attach to a disposable stethoscope cover. Alternatively, the cartridge or cassette may be comprised of disposable instrument covers so that a user may insert the instrument near or through the window of the dispenser for application of the instrument cover directly to the instrument. The kit may further include a proximity sensor for detecting a user.

Abstract: The present invention is a method and compositions for forming a library for nucleic acids sequencing simultaneously from DNA and RNA present in a sample.

Abstract: Metallic substrate treatment methods and articles comprising a phosphonate functionalized layer are provided. The method comprises contacting a metallic substrate comprising at least one of aluminum and an aluminum alloy with a fluid to form a phosphonate functionalized layer on at least a region of the metallic substrate. The fluid comprises at least one of a phosphonate containing acid and a derivative thereof. At least one of the phosphonate containing acid and the derivative thereof comprises a pKa of a first acidic proton. The fluid comprises a pH at least 0.5 pH value greater than the pKa of the first acidic proton. The article comprises a metallic substrate comprising aluminum or an aluminum alloy and a phosphonate functionalized layer on at least a region of the metallic substrate.

Abstract: A bottom pan seal system for sealing a space between a floor of a slide-out room and a floor of a main cabin of a recreational vehicle, the bottom pan seal system having a bottom pan seal having a first leg having an outboard end and an inboard end. A second leg extends perpendicularly from the first leg. A wiper seal extends perpendicularly from the first leg opposite the second leg adjacent the outboard end. A flap seal extending perpendicularly from the second leg. A wear bar can be attached to three inboard end. The inboard end can have a wiper seal extending perpendicularly from the first leg and parallel to the wiper seal adjacent to the outboard end.

Abstract: A medical system comprising a guiding catheter having an inflation lumen. The inflation lumen extends within a handle and a shaft of the guiding catheter. An inflatable sleeve has a proximal end and a distal end, with the ends attached to the guiding catheter. A sealed interior portion of the inflatable sleeve is bounded by the inflatable sleeve and the shaft of the guiding catheter, and in fluid communication with a distal port of the inflation lumen. The system further comprises a delivery catheter defining a delivery lumen and configured for sliding engagement within the guide lumen of the guiding catheter.

Abstract: A method to prevent, inhibit or treat one or more neurological symptoms associated with a central nervous system disorder, e.g. MPSI or MPSII by, for example, intrathecally, intracerebroventricularly or intravenously administering a rAAV encoding a gene product associated with the disease, e.g., administering to an adult mammal in which the gene product is absent, defective or present at a reduced level relative to a mammal without the disease.

Abstract: A slide seal for an RV to seal the space in between the corner of a slide-out room of the RV and a wall of the RV, the slide seal having a base to fasten to the wall, a flap seal extending from the base at one end, a bulb seal extending perpendicular to the base at an opposite end, a horn extending from the bulb seal, curving upwardly from the bulb seal and away from the flap seal, and a notch created through the flap seal and the base to create two sections of the base and flap seal so that the first section of the flap seal and base can be bent at approximately a 90 degree angle relative to the second section of the flap seal and base to fit into the corner space in between the slide-out room and the RV wall.

Abstract: A vehicle seat storage system includes a center passenger seat disposed between side passenger seats. The center seat includes a seat base and a seat back. A reclining mechanism mounted to one of side frame members of the seat base allows the seat back to move relative to the seat base. Seat anchors are disposed outwardly of the side frame members for securing a child seat. The center seat has a seating condition where the center seat is attached to a vehicle floor, and a stowed condition where the center seat is unattached from the floor with the seat back folded against the seat base. A vehicle interior sidewall includes a recessed portion configured to receive the center seat in the stowed condition. A lock mechanism provided in the recessed portion releasably engages the center seat when positioned in the recessed portion.

Abstract: A system and method for broadcast response generally employing RDS, DARC, or similar technology is provided, including a method for responding to a broadcast comprising extracting an event identifier from a broadcast signal; detecting a response by a user to the broadcast signal; polling a communications device to determine a user identifier; and communicating the event identifier and the user identifier when the user response is detected.

Abstract: A broadcast response system provides, e.g., a radio broadcast listener with the ability to obtain media content such as music or speech while listening to the radio. The user can respond to items in the radio broadcast such as advertisements, fund raising drives, or interactive listener polls during the broadcast. Data such as song title and artist, author or publisher and the IP address for the location where the digital version of the content is stored, can be transmitted using, e.g., the RBDS/RDS data stream. A reference number representing song title and artist, author or publisher and the IP address for the location where the digital version of the content is stored can also be employed for ease of implementation. This reference number can reside in a lookup table to be accessed by broadcast response server.

Abstract: The disclosure herein provides systems and methods for a media enhancement system configured to associate a secondary media signal (for example, the secondary media signal can comprise an advertisement) to a primary media signal (for example, a radio broadcast). The disclosure herein additionally provides systems and methods for a media enhancement system that enables the generating, transmitting, displaying, and/or responding to a plurality of associated and/or unassociated secondary media signals, based on a primary media content from a primary media signal, user characteristics (for example, demographic and/or geographic information), and/or third-party preferences (for example, the goals of advertisers). The secondary media signals can be used to enhance the primary media content already being provided to the user on a user device. The secondary media signals can also be used to create psychological associations or relationships with the primary media content already being provided to the user.

Abstract: A piston seal assembly for a gas turbine engine includes a seal composed of a nickel-based superalloy; a component in contact with the seal and defining a seal-counterface; and a coating on the seal at the seal-counterface, wherein the coating is a metal alloy binder phase and a hard particle phase distributed through the binder phase.