Patents by Inventor Kelvin J.A. Davies

Kelvin J.A. Davies has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Labeling of proteins with the fluorophore 7-amino-4-methylcoumarin (AMC) generated novel proteolytic substrates
    Patent number: 9389182
    Abstract: A method of measuring the degradation of intact proteins includes a step of providing a protein substrate having one or more free or exposed carboxyl groups and then reductively attaching 7-amino-4-methylcoumarin (AMC) to the protein substrate with a reducing agent. The protein substrate is contacted in a test solution with one or more proteolytic enzymes that degrade the protein substrate. The amount of AMC attached to the protein substrate is then determined by monitoring the fluorescence of free 7-amino-4-methylcoumarin that is formed during degradation of the protein substrate to protein fragments.
    Type: Grant
    Filed: August 31, 2012
    Date of Patent: July 12, 2016
    Assignee: University of Southern California
    Inventors: Kelvin J. A. Davies, Andrew M. Pickering
  • Labeling of Proteins with the Fluorophore 7-amino-4-methylcoumarin (AMC) Generated Novel Proteolytic Substrates
    Publication number: 20130059321
    Abstract: A method of measuring the degradation of intact proteins includes a step of providing a protein substrate having one or more free or exposed carboxyl groups and then reductively attaching 7-amino-4-methylcoumarin (AMC) to the protein substrate with a reducing agent. The protein substrate is contacted in a test solution with one or more proteolytic enzymes that degrade the protein substrate. The amount of AMC attached to the protein substrate is then determined by monitoring the fluorescence of free 7-amino-4-methylcoumarin that is formed during degradation of the protein substrate to protein fragments.
    Type: Application
    Filed: August 31, 2012
    Publication date: March 7, 2013
    Applicant: UNIVERSITY OF SOUTHERN CALIFORNIA
    Inventors: Kelvin J.A. Davies, Andrew M. Pickering