Patents by Inventor Kemmons A. Tubbs

Kemmons A. Tubbs has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20070128734
    Abstract: Presented herein are methods, devices and kits for the mass spectrometric immunoassay (MSIA) of proteins and their variants that are present in complex biological fluids or extracts. Protein and variant levels can be determined using quantitative methods in which the protein/variant signals are normalized to signals of internal reference standard species (either doped into the samples prior to the MSIA analysis, or other endogenous protein co-extracted with the target proteins) and the values compared to working curves constructed from samples containing known concentrations of the protein or variants.
    Type: Application
    Filed: February 6, 2007
    Publication date: June 7, 2007
    Applicant: INTRINSIC BIOPROBES, INC.
    Inventors: Urban Kiernan, Eric Niederkofler, Kemmons Tubbs, Dobrin Nedelkov, Randall Nelson
  • Publication number: 20070128663
    Abstract: Presented herein are methods, devices and kits for the mass spectrometric immunoassay (MSIA) of proteins and their variants that are present in complex biological fluids or extracts. Protein and variant levels can be determined using quantitative methods in which the protein/variant signals are normalized to signals of internal reference standard species (either doped into the samples prior to the MSIA analysis, or other endogenous protein co-extracted with the target proteins) and the values compared to working curves constructed from samples containing known concentrations of the protein or variants.
    Type: Application
    Filed: February 6, 2007
    Publication date: June 7, 2007
    Applicant: INTRINSIC BIOPROBES, INC.
    Inventors: Urban Kiernan, Eric Niederkofler, Kemmons Tubbs, Dobrin Nedelkov, Randall Nelson
  • Patent number: 7087163
    Abstract: Described is an affinity microcolumn comprising a high surface area material, which has high flow properties and a low dead volume, contained within a housing and having affinity reagents bound to the surface of the high surface area material that are either activated or activatable. The affinity reagents bound to the surface of the affinity microcolumn further comprise affinity receptors for the integration into high throughput analysis of biomolecules.
    Type: Grant
    Filed: August 16, 2004
    Date of Patent: August 8, 2006
    Assignee: Intrinsic Bioprobes, Inc.
    Inventors: Kemmons A. Tubbs, Karl F. Gruber, Randall W. Nelson
  • Patent number: 7087164
    Abstract: Described is an affinity microcolumn comprising a high surface area material, which has high flow properties and a low dead volume, contained within a housing and having affinity reagents bound to the surface of the high surface area material that are either activated or activatable. The affinity reagents bound to the surface of the affinity microcolumn further comprise affinity receptors for the integration into high throughput analysis of biomolecules.
    Type: Grant
    Filed: August 16, 2004
    Date of Patent: August 8, 2006
    Assignee: Intrinsic Bioprobes, Inc.
    Inventors: Kemmons A. Tubbs, Karl F. Gruber, Randall W. Nelson
  • Patent number: 7087165
    Abstract: Described is an affinity microcolumn comprising a high surface area material, which has high flow properties and a low dead volume, contained within a housing and having affinity reagents bound to the surface of the high surface area material that are either activated or activatable. The affinity reagents bound to the surface of the affinity microcolumn further comprise affinity receptors for the integration into high throughput analysis of biomolecules.
    Type: Grant
    Filed: August 16, 2004
    Date of Patent: August 8, 2006
    Assignee: Intrinsic Bioprobes, Inc.
    Inventors: Kemmons A Tubbs, Karl F. Gruber, Randall W. Nelson
  • Patent number: 7083723
    Abstract: Described is an affinity microcolumn comprising a high surface area material, which has high flow properties and a low dead volume, contained within a housing and having affinity reagents bound to the surface of the high surface area material that are either activated or activatable. The affinity reagents bound to the surface of the affinity microcolumn further comprise affinity receptors for the integration into high throughput analysis of biomolecules.
    Type: Grant
    Filed: August 16, 2004
    Date of Patent: August 1, 2006
    Assignee: Intrinsic Bioprobes, Inc.
    Inventors: Kemmons A. Tubbs, Karl F. Gruber, Randall W. Nelson
  • Patent number: 7083724
    Abstract: Described is an affinity microcolumn comprising a high surface area material, which has high flow properties and a low dead volume, contained within a housing and having affinity reagents bound to the surface of the high surface area material that are either activated or activatable. The affinity reagents bound to the surface of the affinity microcolumn further comprise affinity receptors for the integration into high throughput analysis of biomolecules.
    Type: Grant
    Filed: August 16, 2004
    Date of Patent: August 1, 2006
    Assignee: Intrinsic Bioprobes, Inc.
    Inventors: Kemmons A. Tubbs, Karl F. Gruber, Randall W. Nelson
  • Publication number: 20060127948
    Abstract: Presented herein are methods, devices and kits for the mass spectrometric immunoassay (MSIA) of proteins present in complex biological fluids or extracts. Pipettor tips containing porous solid supports that are covalently derivatized with affinity ligand and used to extract specific proteins and their variants from various biological fluids. Nonspecifically bound compounds are rinsed from the extraction devices using a series of buffer and water rinses, after which the wild type protein (and/or its variants) are eluted directly onto a target in preparation for analysis such as matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). Mass spectrometry of the eluted sample then follows with the retained proteins identified via accurate molecular mass determination.
    Type: Application
    Filed: October 24, 2005
    Publication date: June 15, 2006
    Applicant: INTRINSIC BIOPROBES, INC.
    Inventors: Urban Kiernan, Eric Niederkofler, Kemmons Tubbs, Dobrin Nedelkov, Randall Nelson
  • Publication number: 20060121533
    Abstract: Presented herein are methods, devices and kits for the mass spectrometric immunoassay (MSIA) of proteins present in complex biological fluids or extracts. Pipettor tips containing porous solid supports that are covalently derivatized with affinity ligand and used to extract specific proteins and their variants from various biological fluids. Nonspecifically bound compounds are rinsed from the extraction devices using a series of buffer and water rinses, after which the wild type protein (and/or its variants) are eluted directly onto a target in preparation for analysis such as matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). Mass spectrometry of the eluted sample then follows with the retained proteins identified via accurate molecular mass determination.
    Type: Application
    Filed: October 24, 2005
    Publication date: June 8, 2006
    Applicant: INTRINSIC BIOPROBES, INC.
    Inventors: Urban Kiernan, Eric Niederkofler, Kemmons Tubbs, Dobrin Nedelkov, Randall Nelson
  • Publication number: 20050164409
    Abstract: Presented herein are methods, devices and kits for the mass spectrometric immunoassay (MSIA) of proteins and their variants that are present in complex biological fluids or extracts. Protein and variant levels can be determined using quantitative methods in which the protein/variant signals are normalized to signals of internal reference standard species (either doped into the samples prior to the MSIA analysis, or other endogenous protein co-extracted with the target proteins) and the values compared to working curves constructed from samples containing known concentrations of the protein or variants.
    Type: Application
    Filed: December 10, 2004
    Publication date: July 28, 2005
    Inventors: Urban Kiernan, Eric Niederkofler, Kemmons Tubbs, Dobrin Nedelkov, Randall Nelson
  • Publication number: 20050032116
    Abstract: Presented herein are affinity-based mass spectrometric methods and assays for analysis of insulin like growth factors 1 and 2 (IGF-1 and IGF-2) present in complex biological mixtures and fluids. IGF-1 and IGF-2 were assayed from human plasma via BIA/MS, utilizing antibodies as ligands for affinity retrieval. Detection of both targeted and non-targeted IGFs in the mass spectra indicated possible protein complex retrieval by the individual antibodies. Plasma samples were investigated under variable denaturing conditions to confirm the detection of both free and bound IGFs. In a MSIA approach to IGF detection, pipettor tips containing porous solid supports covalently derivatized with anti-IGF antibodies were used to extract specific IGFs from plasma in preparation for mass spectrometry. Single or multiplex IGF-1 and IGF-2 assays were performed, resulting in detection of wild-type IGF-1 and 2, and a truncated IGF-2 variant, missing its N-terminal Alanine (also detected in the BIA/MS experiments).
    Type: Application
    Filed: January 12, 2004
    Publication date: February 10, 2005
    Inventors: Randall Nelson, Dobrin Nedelkov, Urban Kiernan, Kemmons Tubbs
  • Publication number: 20050003372
    Abstract: Described is an affinity microcolumn comprising a high surface area material, which has high flow properties and a low dead volume, contained within a housing and having affinity reagents bound to the surface of the high surface area material that are either activated or activatable. The affinity reagents bound to the surface of the affinity microcolumn further comprise affinity receptors for the integration into high throughput analysis of biomolecules.
    Type: Application
    Filed: August 16, 2004
    Publication date: January 6, 2005
    Inventors: Kemmons Tubbs, Karl Gruber, Randall Nelson
  • Publication number: 20050003373
    Abstract: Described is an affinity microcolumn comprising a high surface area material, which has high flow properties and a low dead volume, contained within a housing and having affinity reagents bound to the surface of the high surface area material that are either activated or activatable. The affinity reagents bound to the surface of the affinity microcolumn further comprise affinity receptors for the integration into high throughput analysis of biomolecules.
    Type: Application
    Filed: August 16, 2004
    Publication date: January 6, 2005
    Inventors: Kemmons Tubbs, Karl Gruber, Randall Nelson
  • Publication number: 20050003555
    Abstract: Described is an affinity microcolumn comprising a high surface area material, which has high flow properties and a low dead volume, contained within a housing and having affinity reagents bound to the surface of the high surface area material that are either activated or activatable. The affinity reagents bound to the surface of the affinity microcolumn further comprise affinity receptors for the integration into high throughput analysis of biomolecules.
    Type: Application
    Filed: August 16, 2004
    Publication date: January 6, 2005
    Inventors: Kemmons Tubbs, Karl Gruber, Randall Nelson
  • Publication number: 20040265843
    Abstract: Described is an affinity microcolumn comprising a high surface area material, which has high flow properties and a low dead volume, contained within a housing and having affinity reagents bound to the surface of the high surface area material that are either activated or activatable. The affinity reagents bound to the surface of the affinity microcolumn further comprise affinity receptors for the integration into high throughput analysis of biomolecules.
    Type: Application
    Filed: August 16, 2004
    Publication date: December 30, 2004
    Inventors: Kemmons A. Tubbs, Karl F. Gruber, Randall W. Nelson
  • Publication number: 20040265844
    Abstract: Described is an affinity microcolumn comprising a high surface area material, which has high flow properties and a low dead volume, contained within a housing and having affinity reagents bound to the surface of the high surface area material that are either activated or activatable. The affinity reagents bound to the surface of the affinity microcolumn further comprise affinity receptors for the integration into high throughput analysis of biomolecules.
    Type: Application
    Filed: August 16, 2004
    Publication date: December 30, 2004
    Inventors: Kemmons A. Tubbs, Karl F. Gruber, Randall W. Nelson
  • Patent number: 6783672
    Abstract: Described is an affinity microcolumn comprising a high surface area material, which has high flow properties and a low dead volume, contained within a housing and having affinity reagents bound to the surface of the high surface area material that are either activated or activatable. The affinity reagents bound to the surface of the affinity microcolumn further comprise affinity receptors for the integration into high throughput analysis of biomolecules.
    Type: Grant
    Filed: January 15, 2002
    Date of Patent: August 31, 2004
    Inventors: Kemmons A. Tubbs, Karl F. Gruber, Randall W. Nelson
  • Publication number: 20030027216
    Abstract: Presented herein are methods, devices and kits for the mass spectrometric immunoassay (MSIA) of proteins present in complex biological fluids or extracts Pipettor tips containing porous solid supports that are covalently derivatized with affinity ligand and used to extract specific proteins and their variants from various biological fluids Nonspecifically bound compounds are rinsed from the extraction devices using a series of buffer and water rinses, after which the wild type protein (and/or its variants) are eluted directly onto a target in preparation for analysis such as matrix-assisted laser desorption/ionization time-of-light mass spectrometry (MALDI-TOF MS). Mass spectrometry of the eluted sample then follows with the retained proteins identified via accurate molecular mass determination.
    Type: Application
    Filed: July 2, 2002
    Publication date: February 6, 2003
    Inventors: Urban A. Kiernan, Eric Niederkofler, Kemmons A. Tubbs, Dobrin Nedelkov, Randall W. Nelson
  • Publication number: 20020164818
    Abstract: Presented herein is the construction of pipettor tips (termed MSIA-Tips) containing porous solid supports that are constructed, covalently derivatized with affinity ligand, and used to extract specific proteins and their variants from various biological fluids by repeatedly flowing the fluids through the MSIA-Tips. A second protein species (a mass-shifted variant of the targeted protein doped into the samples at a constant concentration) is co-extracted with the endogenous protein and variants and is used as a quantitative internal reference standard (IRS). Nonspecifically bound compounds are rinsed from the MSIA-Tip using a series of buffer and water rinses, after which the wild type protein, protein variants and the IRS are eluted from the MSIA-Tips directly onto a target in preparation for analysis such as MALDI-TOF. Mass spectrometry of the eluted sample then follows with the retained proteins identified via accurate molecular mass determination.
    Type: Application
    Filed: May 14, 2001
    Publication date: November 7, 2002
    Inventors: Karl F. Gruber, Kemmons A. Tubbs, Randall W. Nelson
  • Publication number: 20020110904
    Abstract: The present invention provides an integrated system capable of selectively retrieving and concentrating specific biomolecules from biological media for subsequent high-performance analyses, quantifying targeted proteins, recognizing variants of targeted biomolecules (e.g., splice variants, point mutations and post-translational modifications) and elucidating their nature, analyzing for, and identifying, ligands interacting with targeted biomolecules, and high-throughput screening of large populations of samples using a single, unified, economical, multiplexed and parallel processing platform. The preferred embodiment of the integrated system comprises molecular traps, such as affinity microcolumns, derivatized mass spectrometer targets, mass spectrometers capable of multi-sample input and robotics with processing/data analysis interactive database. The present invention also includes methods and processes for use of the individual components and the integrated system in biological applications.
    Type: Application
    Filed: January 15, 2002
    Publication date: August 15, 2002
    Inventors: Randall W. Nelson, Kemmons A. Tubbs, Karl A. Gruber