Abstract: A safety cabinet has a second operation chamber in a first operation chamber and into which clean air is supplied, a second suction opening for sucking in air in the second operation chamber and a portion of the air in the first operation chamber, and a second operation opening part that is provided facing a first operation opening part and communicates the second operation chamber with the first operation chamber. Airflow containment (air barrier) is doubled with respect to the outside of a safety cabinet. In cases where decontamination and disinfection operations are carried out according to the changeover procedures, intensive decontamination and disinfection of the second operation chamber is sufficient, and decontamination and disinfection of the first operation chamber is hardly required. Decontamination and disinfection operations in the changeover procedures and the like can be carried out in a greatly reduced time.

Abstract: A work area is disposed in a conveyance path for cells and has an inlet and an outlet, with a downflow air curtain provided at least at the inlet and the outlet. A conveyor repeatedly performs an operation that moves a placement surface having a culture vessel thereon from the inlet side into the area, moves the placement surface from the outlet side to the outside of the area, and, subsequently, moves back the placement surface to the inlet side. The cleaning device cleans the placement surface during the period from when the conveyor in the work area moves the placement surface to the outside of the area until the conveyor moves back the placement surface to the inlet side of the area.

Abstract: A safety cabinet has a second operation chamber in a first operation chamber and into which clean air is supplied, a second suction opening for sucking in air in the second operation chamber and a portion of the air in the first operation chamber, and a second operation opening part that is provided facing a first operation opening part and communicates the second operation chamber with the first operation chamber. Airflow containment (air barrier) is doubled with respect to the outside of a safety cabinet. In cases where decontamination and disinfection operations are carried out according to the changeover procedures, intensive decontamination and disinfection of the second operation chamber is sufficient, and decontamination and disinfection of the first operation chamber is hardly required. Decontamination and disinfection operations in the changeover procedures and the like can be carried out in a greatly reduced time.

Abstract: A work area is disposed in a conveyance path for cells and has an inlet and an outlet, with a downflow air curtain provided at least at the inlet and the outlet. A conveyor repeatedly performs an operation that moves a placement surface having a culture vessel thereon from the inlet side into the area, moves the placement surface from the outlet side to the outside of the area, and, subsequently, moves back the placement surface to the inlet side. The cleaning device cleans the placement surface during the period from when the conveyor in the work area moves the placement surface to the outside of the area until the conveyor moves back the placement surface to the inlet side of the area.

Abstract: Artificial dermis for transplantation includes a wound contact layer that includes a porous sheet consisting of a biodegradable material, the porous sheet satisfying the following Equation, Equation: X2/X1?0.8. In the porous sheet, when a line parallel to a wound contact face and a line perpendicular to a wound contact face of equal length are drawn, X1 represents a number of points of intersection between the line parallel to the wound contact face and pore walls; and X2 represents a number of points of intersection between the line perpendicular to the wound contact face and pore walls.

Abstract: A method for manufacturing at least one kind of eukaryotic cells or a biosynthetic substance derived from the eukaryotic cells by proliferating the cells, which includes preparing an artificially synthesized peptide for promoting proliferation of the at least one kind of eukaryotic cells, incubating the eukaryotic cells in a culture medium, and adding the synthesized peptide at least once to the culture medium during the incubation process. The synthesized peptide includes an amino acid sequence selected from SEQ ID NOS: 1 to 18, and an amino acid sequence selected from SEQ ID NOS: 19 to 97.

Abstract: A method for manufacturing at least one kind of eukaryotic cells or a biosynthetic substance derived from the eukaryotic cells by proliferating the cells, which includes preparing an artificially synthesized peptide for promoting proliferation of the at least one kind of eukaryotic cells, incubating the eukaryotic cells in a culture medium, and adding the synthesized peptide at least once to the culture medium during the incubation process. The synthesized peptide includes an amino acid sequence selected from SEQ ID NOS: 1 to 18, and an amino acid sequence selected from SEQ ID NOS: 19 to 97.

Abstract: A cell proliferation promoter includes, as an active ingredient, an artificially synthesized peptide that includes (A) an amino acid sequence constituting a membrane-permeable peptide and (B) an amino acid sequence selected from SEQ ID NOs: 19 to 103 or an amino acid sequence formed by substituting, deleting and/or adding one or several amino acid residues in the selected amino acid sequence.

Abstract: A cell proliferation promoter includes, as an active ingredient, an artificially synthesized peptide that includes (A) an amino acid sequence constituting a membrane-permeable peptide and (B) an amino acid sequence selected from SEQ ID NOs: 19 to 103 or an amino acid sequence formed by substituting, deleting and/or adding one or several amino acid residues in the selected amino acid sequence.

Abstract: Under a state where the zipper 22 of a culture bag is opened, an operator depresses the opening button 43 of an arm 40 with one finger. Consequently, a force for oscillating the arm 40 to separate a retaining portion 49 upward from the culture surface 32 around a pin 42 acts against the urging force of a leaf spring 50. Consequently, the tip 52a of an extended portion 52 ascends to a predetermined position while resisting against the urging force of the leaf spring 50. Since the lower surface 20a of the culture bag 20 is retained by a base 31 and the upper surface 20b is raised upward by the tip 52a of an extended portion 52, opening 24 of the culture bag 20 is widened. Since the width of the base 31 is larger than that of the arm 40, upper space of the culture surface 32 is not occupied by the arm 40 and a sufficiently wide work space is provided in the culture bag 20.

Abstract: A cell culture method prepares first cells that are adhesion-dependent cells and monolayered or multilayered cells on a culture surface of a culture substrate, seeds second cells that are adhesion-dependent cells and are magnetized by allowing to have magnetic particles on the first cells, induces the second cells to a predetermined position on the first cells by magnetic force, and cultures the first cells and the second cells in a cell arrangement obtained by the magnetic induction. According to this cell culture method, after a cell sheet was prepared individually, cells can be multilayered without changing a temperature, peeling the monolayered sheet and laminating the monolayered sheets.

Abstract: Epidermal keratinocytes not incorporating magnetic particles therein and epidermal keratinocytes incorporating magnetic particles therein were seeded in a 24-well ultra-low-attachment plate, and cultured for one day after a neodymium magnet was placed outside the bottom of the well. As a result, control epidermal keratinocytes not incorporating magnetic particles therein did not adhere to the bottom of the well and did not form an undifferentiated cell sheet. On the contrary, epidermal keratinocytes incorporating magnetic particles therein, while being attracted to the bottom of the well by magnetic force, formed a multilayered cell sheet. This cell sheet could be easily recovered by removing the neodymium magnet placed outside the bottom of the well, then bringing a magnet having a hydrophilic film attached thereto close to the cell sheet from upside and to attract it via this hydrophilic film, and lifting it.

Abstract: This invention relates to an instrument for regenerating a living organism tissue or organ, characterized in that a support (A) formed from a biodegradable material or a bioabsorbable material includes a sponge-like fine matrix (B) formed from a biodegradable material or a bioabsorbable material and a linear guide channel (C) for a living organism tissue or organ.

Abstract: This invention relates to an instrument for regenerating a living organism tissue or organ, characterized in that a support (A) formed from a biodegradable material or a bioabsorbable material includes a sponge-like fine matrix (B) formed from a biodegradable material or a bioabsorbable material and a linear guide channel (C) for a living organism tissue or organ.