Patents by Inventor Ken Okabayashi

Ken Okabayashi has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Albumin gene-containing plasmid, transformant carrying same, production of such transformant and production of albumin
    Patent number: 5756313
    Abstract: A plasmid for a yeast host, the plasmid comprising in sequence: (1) a yeast derived promoter, (2) an albumin-encoding region placed under control of the yeast-derived promoter, (3) a transcription terminator and (4) a sequence homologous to a part of the yeast host chromosomal sequence such that the plasmid is capable of being integrated into the yeast host cell chromosome and wherein the plasmid is incapable of autonomous replication in yeast cells; a yeast host transformed with the above plasmid; a method of producing a yeast transformant which comprises transforming a yeast host by integrating at least two different plasmids each having a sequence homologous to a part of the host yeast chromosomal sequence one at a time into the chromosome of the yeast host cell; and a method of producing albumin which comprises cultivating the above transformant and recovering the thus-produced albumin.
    Type: Grant
    Filed: October 20, 1993
    Date of Patent: May 26, 1998
    Assignee: The Green Cross Corporation
    Inventors: Ken Okabayashi, Hideyuki Oi, Kazumoto Hirabayashi, Masami Miura, Miho Shimizu, Haruhide Kawabe
  • Mutant strain of Pichia pastoris which utilizes methanol in the presence of glucose
    Patent number: 5643792
    Abstract: A methylotrophic and glucotrophic mutant strain capable of producing a heterologous protein and a method for producing a heterologous protein, comprising culture of the mutant strain. The mutant strain of the present invention can be grown in a medium containing both methanol and glucose, with the effect that the growth of the strain and production of a heterologous protein proceed at the same time. Accordingly, a heterologous protein can be produced in a large amount in a short time.
    Type: Grant
    Filed: January 13, 1994
    Date of Patent: July 1, 1997
    Assignee: The Green Cross Corporation
    Inventors: Ken Okabayashi, Takao Ohmura, Kazumasa Yokoyama, Haruhide Kawabe
  • Method of preparing foreign protein in yeast, recombinant DNA, transformant
    Patent number: 5503993
    Abstract: A method for preparing foreign protein in yeast using an expression recombinant DNA comprising DNA encoding the serum albumin signal peptide adjacent to DNA encoding the foreign protein is disclosed.
    Type: Grant
    Filed: May 22, 1995
    Date of Patent: April 2, 1996
    Assignee: The Green Cross Corporation
    Inventors: Naofumi Hayasuke, Yukimitsu Nakagawa, Yutaka Ishida, Ken Okabayashi, Kohji Murakami, Kiyoshi Tsutsui, Kazuo Ikegaya, Hitoshi Minamino, Sadao Ueda, Haruhide Kawabe, Hirofumi Arimura, Atusi Masaki
  • DNA's encoding signal peptides
    Patent number: 5409815
    Abstract: Novel signal peptides capable of functioning in yeasts have the amino acid sequence:Met-A.sub.1 -A.sub.2 -X-B-C-D-E-Fwherein A.sub.1 is a peptide chain composed of 1-3 amino acids each selected from the group consisting of Arg, Ser, Lys and His, A.sub.2 is a peptide chain composed of 1-3 amino acids, X is a peptide chain composed of 8-10 hydrophobic amino acids, B is Pro or Ser, C is Gly or Pro, D Cys, Ala, Leu, Ser, Thr and Val, E is Trp or Gln and F is Ala or Gly. DNA sequences coding for these signal peptides can be used for the secretory expression of heterologous proteins in yeasts.
    Type: Grant
    Filed: February 16, 1989
    Date of Patent: April 25, 1995
    Assignee: The Green Cross Corporation
    Inventors: Yukimitsu Nakagawa, Naofumi Hayasuke, Atsushi Masaki, Yutaka Ishida, Kohji Murakami, Ken Okabayashi, Kiyoshi Tsutsui, Hitoshi Minamino, Kazuo Ikegaya, Kaoru Kobayashi, Haruhide Kawabe, Sadao Ueda