Abstract: The present invention concerns a method of genetic modification of a TGB-3 wild type viral sequence for reducing or suppressing the possible deleterious effects of the agronomic properties of a transformed plant or plant cell by said TGB-3 viral sequence. The invention further relates to genetically modified TGB-3 viral sequences suitable to induce gene silencing. In particular hairpin constructs based on such sequences proved highly efficient to induce a PTGS mechanism and degradation of the whole of RNA2 thereby. When plants are transformed accordingly the spread of the virus in the plant is significantly reduced or blocked.

Abstract: The present invention concerns a method of genetic modification of a TGB-3 wild type viral sequence for reducing or suppressing the possible deleterious effects of the agronomic properties of a transformed plant or plant cell by said TGB-3 viral sequence. The invention further relates to genetically modified TGB-3 viral sequences suitable to induce gene silencing. In particular hairpin constructs based on such sequences proved highly efficient to induce a PTGS mechanism and degradation of the whole of RNA2 thereby. When plants are transformed accordingly the spread of the virus in the plant is significantly reduced or blocked.

Abstract: The present invention concerns a method of genetic modification of a TGB-3 wild type viral sequence for reducing or suppressing the possible deleterious effects of the agronomic properties of a transformed plant or plant cell by said TGB-3 viral sequence, comprising the following successive steps: submitting said sequence to point mutation(s) which allow the substitution of at least one amino-acid into a different amino-acid; selecting genetically modified TGB-3 wild type viral sequences having said point mutation(s) and which are not able to promote cell-to-cell movement of a mutant virus having a dysfunctional TGB-3 wild type viral sequence, when expressed in trans from a replicon; further selecting among said genetically modified TGB-3 viral sequences, the specifically genetically modified sequence which inhibits infection with a co-inoculated wild type virus when the mutant form was expressed from a replicon, and recovering said specifically genetically modified TGB-3 viral sequence.

Abstract: The present invention comprises a circuit and method for controlling current to a load. In an exemplary embodiment, the circuit makes the maximum charge time (“MCT”) of a battery charging circuit (100) a function of the charging current. The MCT changes as the battery charging current changes. For low current the MCT would increase and for high current the MCT would decrease. The result is that MCT will always occur a short time after the battery is fully charged. In operation, the charging current can also be made a function of the temperature of devices within the charging circuit.

Abstract: The present invention concerns a method of genetic modification of a TGB-3 wild type viral sequence for reducing or suppressing the possible deleterious effects of the agronomic properties of a transformed plant or plant cell by said TGB-3 viral sequence, comprising the following successive steps: submitting said sequence to point mutation(s) which allow the substitution of at least one amino-acid into a different amino-acid; selecting genetically modified TGB-3 wild type viral sequences having said point mutation(s) and which are not able to promote cell-to-cell movement of a mutant virus having a dysfunctional TGB-3 wild type viral sequence, when expressed in trans from a replicon; further selecting among said genetically modified TGB-3 viral sequences, the specifically genetically modified sequence which inhibits infection with a co-inoculated wild type virus when the mutant form was expressed from a replicon; and recovering said specifically genetically modified TGB-3 viral sequence.

Abstract: The present invention concerns a method of genetic modification of a TGB-3 wild type viral sequence for reducing or suppressing the possible deleterious effects of the agronomic properties of a transformed plant or plant cell by the TGB-3 viral sequence, comprising the following successive steps: submitting the sequence to point mutation(s) which allow the substitution of at least one amino-acid into a different amino-acid, selecting genetically modified TGB-3 wild type viral sequences having the point mutation(s) and which are not able to promote cell-to-cell movement of a mutant virus having a dysfunctional TGB-3 wild type viral sequence, when expressed in trans from a replicon, further selecting among the genetically modified TGB-3 viral sequences, the specifically genetically modified sequence which inhibits infection with a co-inoculated wild type virus when the mutant form was expressed from a replicon, and recovering the specifically genetically modified TGB-3 viral sequence.

Abstract: The present invention concerns a method for inducing resistance to a virus comprising a TGB3 sequence with the proviso that it is not the potato virus X, into a plant cell or plant, comprising the following steps: preparing a nucleic acid construct comprising a nucleic acid sequence corresponding to at least 70% of the nucleic acid sequence of TGB3 of said virus or its corresponding cDNA, being operably linked to one or more regulatory sequence(s) active in a plant, transforming a plant cell with the nucleic acid construct, and possibly regenerating a transgenic plant from the transformed plant cell. The present invention is also related to the plant obtained.

Abstract: A control circuit is provided which includes a single programmable terminal for controlling a plurality of modes, functions or parameters in a programmable circuit with a minimum of program elements connected to the single programmable terminal. The program elements may illustratively be resistors, capacitors, inductors or other circuit components. In a first mode, for example, two program elements control a signal generating function in the programmable circuit. In a second mode, a voltage provided internally forces a condition at the programmable terminal to control another signal generating function. In both first and second modes, the values of the program elements, selectable by the user, also determine the particular frequencies of the respective generated signals. In a third mode, the signals generated in the first and second modes are compared to provide a control function.

Abstract: A control circuit is provided which includes a single programmable terminal for controlling a plurality of modes, functions or parameters in a programmable circuit with a minimum of program elements connected to the single programmable terminal. The program elements may illustratively be resistors, capacitors, inductors or other circuit components. In a first mode, for example, two program elements control a signal generating function in the programmable circuit. In a second mode in this example, a voltage provided internally forces a condition at the programmable terminal to control another signal generating function. In both first and second modes, the values of the program elements, selectable by the user, also determine the particular frequencies of the respective generated signals. In a third mode, the signals generated in the first and second modes are compared to provide yet another a control function.