Abstract: Provided herein are approaches for the detection, identification, and/or selective amplification of specific target species or target variants of nucleic acid sequences, even within an excess of unwanted similar sequences or variants. These approaches include methods, assays, and kits for suppression PCR that require, in part, DNA polymerase that lacks 5?-3? exonuclease activity, and a PCR primer, termed a forward selective primer or a nunchaku primer. The methods, assays, and kits provided herein are useful for a wide variety of applications, including cancer screening assays and kits, personalized screening assays, SNP (single nucleotide polymorphism) genotyping and identification, and downstream applications such as next generation high-throughput genomic sequencing and library construction.

Abstract: Provided herein are approaches for the detection, identification, and/or selective amplification of specific target species or target variants of nucleic acid sequences, even within an excess of unwanted similar sequences or variants. These approaches include methods, assays, and kits for suppression PCR that require, in part, DNA polymerase that lacks 5?-3? exonuclease activity, and a PCR primer, termed a forward selective primer or a nunchaku primer. The methods, assays, and kits provided herein are useful for a wide variety of applications, including cancer screening assays and kits, personalized screening assays, SNP (single nucleotide polymorphism) genotyping and identification, and downstream applications such as next generation high-throughput genomic sequencing and library construction.

Abstract: An axle beam suspension arrangement comprises a lateral link assembly which is mounted to a single support point on a vehicle body and two support points on an axle beam of the vehicle. The lateral link assembly consists of a lateral link member connected between a support point on the axle beam and the support point on the vehicle body, an assist link is provided which connects between the second support point on the axle beam and a point on the length of the lateral link, closer to the end at which the lateral link connects to the axle than the end connecting to the vehicle body. The lateral link is connected to the axle beam support point via a bush which is flexible in the axial direction of the lateral link. Thus scuff, or vertical disalignment of the vehicle wheels during bounding and rebounding is prevented while occurrence of rolling may be detected while jack-up forces applied to vehicle wheels are absorbed.