Abstract: A transcript mapping method according to an embodiment of the invention is described hereinafter and combines short tag based (SAGE and MPSS) efficiency with the accuracy of full-length cDNA (flcDNA) for comprehensive characterization of transcriptomes. This method is also referred to as Gene Identification Signature (GIS) analysis. In this method, the 5? and 3? ends of full-length cDNA clones are initially extracted into a ditag structure, with the ditag concatemers of the ditag being subsequently sequenced in an efficient manner, and finally mapped to the genome for defining the gene structure. As a GIS ditag represents the 5? and 3? ends of a transcript, it is more informative than SAGE and MPSS tags. Segment lengths between 5? and 3? tag pairs are obtainable including orientation, ordering and chromosome family for efficient transcript mapping and gene location identification.

Abstract: A transcript mapping method according to an embodiment of the invention is described hereinafter and combines short tag based (SAGE and MPSS) efficiency with the accuracy of full-length cDNA (flcDNA) for comprehensive characterization of transcriptomes. This method is also referred to as Gene Identification Signature (GIS) analysis. In this method, the 5? and 3? ends of full-length cDNA clones are initially extracted into a ditag structure, with the ditag concatemers of the ditag being subsequently sequenced in an efficient manner, and finally mapped to the genome for defining the gene structure. As a GIS ditag represents the 5? and 3? ends of a transcript, it is more informative than SAGE and MPSS tags. Segment lengths between 5? and 3? tag pairs are obtainable including orientation, ordering and chromosome family for efficient transcript mapping and gene location identification.

Abstract: A transcript mapping method according to an embodiment of the invention is described hereinafter and combines short tag based (SAGE and MPSS) efficiency with the accuracy of full-length cDNA (flcDNA) for comprehensive characterization of transcriptomes. This method is also referred to as Gene Identification Signature (GIS) analysis. In this method, 5? and 3? terminal tags are obtained from a transcript and the terminal tags are for identifying 5? and 3? sites located on a genome sequence. Further, occurring segments and thus feasible gene locations are identified along the genome sequence after the 5? and 3? sites are identified. A data structure is also generated for indexing the genome sequence so that the terminal tags can be mapped to the genome sequence.

Abstract: An infant feeding apparatus is formed of a filled reservoir, sealed and coupled to a nipple by way of a special coupling element. Gentle tactile manipulation breaks a seal and creates a flow path such that the contents of the reservoir are permitted to pass through the nipple tip. As the package is made of discardable materials it is expendable after use. These packages are designed as one-time use, disposable packages. They are prepared with integrated food; ready for consumption. In best versions, a highly unique cap and nipple design enables a plurality of functions desirable in packages of this nature. These systems include: a cap which drives various function related to flow control; a specialized nipple element; a base element provides a coupling to both the reservoir envelope containing food and to the cap which maintains a sanitized seal but is removable and replaceable under simple tactile manipulation.

Abstract: An antenna assembly for implementing an antenna internally in a mobile communications device. The antenna assembly includes a substantially planar antenna having terminals extending substantially perpendicular outward from the antenna and disposed adjacent and opposite one another. The antenna assembly also includes a connector having conducting surfaces that are disposed substantially parallel, on opposite surfaces of the connector. The connector may be mounted on a spacer that is mounted in the communications device, with the connector and conducting surfaces substantially perpendicular to the spacer. The antenna is placed on the spacer with the terminals of the antenna extending in the direction of the spacer along both sides of the connector and the terminals each contact one of the conducting surfaces of the connector. The antenna assembly may be secured in the device using a cover congruent with the outer covering of the device.

Abstract: A new category of mobile communications antenna is implemented in a single layer of conducting material. Wire-slot sections, including wire-tabs defining slots in the material, partially extend around the perimeter of at least one patch-tab section of the antenna. The perimeter of the at least one patch-tab section forms one edge of each slot, and the wire-tab of a wire-slot section forms a second edge of the slot. The wire-tabs of the wire-slot sections are separated from the patch-tab section by the slots and merge into the patch-tab section at a desired point. The length of each of the wire-slot sections may vary. A portion of each of a pair of the wire-tabs of the wire-slot sections functions as an input feed. The patch-tab section may be implemented as a single tab or as a plurality of tabs separated from one another by a slot.

Abstract: A fluid level controller having a thermal expansion element in the form of cylinder made from polytetrafluoroethylene. The cylinder is located in a container which is connected to a supply of cryogenic fluid by a ball valve. When the cryogenic fluid in the container reaches the level of the cylinder and cools it, the cylinder shrinks and causes a lever to close the ball valve. When the cryogenic fluid falls below the level of the cylinder, the cylinder expands and the lever opens the ball valve and permits cryogenic fluid to flow through the valve and into the container. The cylinder has a heating element disposed in its interior to hasten the response of the cylinder to a change in the fluid level.