Abstract: A portable toilet particularly suited to be a roll-out toilet positions a toilet with its holding tank at a rear wall, a sink at an opposed front wall, a urinal on one of the side walls and an access door through the opposing sidewall to provide access to the enclosure formed by the, front, rear and side walls. By connecting the drain from the sink to the urinal so that the water draining from the sink must pass through the urinal and then pass from the urinal to a toilet holding tank via a drain line connecting the urinal to a toilet holding tank the sink drain water is collected and will tend to wash and thereby clean the urinal.

Abstract: A portable toilet particularly suited to be a roll-out toilet positions a toilet with its holding tank at a rear wall, a sink at an opposed front wall , a urinal on one of the side walls and an access door through the opposing sidewall to provide access to the enclosure formed by the, front, rear and side walls. By connecting the drain from the sink to the urinal so that the water draining from the sink must pass through the urinal and then pass from the urinal to a toilet holding tank via a drain line connecting the urinal to a toilet holding tank the sink drain water is collected and will tend to wash and thereby clean the urinal .

Abstract: A polynucleotide encoding chitin synthase (CHS1), an enzyme essential for cell wall synthesis and yeast cell growth, is provided. A maltose responsive promoter (MRP) isolated using the promoter library of the invention is also described.The present invention also provides a vector for isolation of a eukaryotic regulatory polynucleotide, i.e., promoter. The vector is useful in the method of the invention which comprises identifying a eukaryotic regulatory polynucleotide, i.e., promoter region, by complementing the growth of an auxotrophic host cell containing the vector of the invention, which includes a promoter region operably linked to a promoterless auxotrophic gene. The vector is introduced into the host cell chromosome by targeted integration. Also provided is a library containing host cells having the vector of the invention integrated in the chromosome of the host cell.

Abstract: A polynucleotide encoding chitin synthase (CHS1), an enzyme essential for cell wall synthesis and yeast cell growth, is provided. A maltose responsive promoter (MRP) isolated using the promoter library of the invention is also described.The present invention also provides a vector for isolation of a eukaryotic regulatory polynucleotide, i.e., promoter. The vector is useful in the method of the invention which comprises identifying a eukaryotic regulatory polynucleotide, i.e., promoter region, by complementing the growth of an auxotrophic host cell containing the vector of the invention, which includes a promoter region operably linked to a promoterless auxotrophic gene. The vector is introduced into the host cell chromosome by targeted integration. Also provided is a library containing host cells having the vector of the invention integrated in the chromosome of the host cell.