Patents by Inventor Kenji Mitsushima

Kenji Mitsushima has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Cephalosporin acetylhydrolase gene and protein encoded by said gene
    Patent number: 5338676
    Abstract: A cephalosporin acetylhydrolase gene, a recombinant DNA molecule prepared by introducing said gene into a vector used in an E. coli host-vector system, E. coli cells transfomed with said recombinant DNA molecule, a protein having the amino acid sequence encoded by said gene, an enzyme which is a multimer of said protein, and a process for preparing said protein or enzyme are provided, said cephalosporin acetylhydrolase being an enzyme useful for converting cephalosporins such as cephalosporin C and 7-ACA into deacetylated ones such as deacetylcephalosporin C and deacetyl 7-ACA which are useful as an intermediate for preparing a variety of derivatized cephalosporin antibiotics.
    Type: Grant
    Filed: November 25, 1992
    Date of Patent: August 16, 1994
    Assignee: Shionogi & Co., Ltd.
    Inventors: Kenji Mitsushima, Akio Takimoto, Shigeo Yaqi, Takayasu Sonoyama
  • Cephalosporin acetylhydrolase gene from Bacillus subtilis
    Patent number: 5281525
    Abstract: A cephalosporin acetylhydrolase gene, a recombinant DNA molecule prepared by introducing said gene into a vector used in an E. coli host-vector system, E. coli cells transformed with said recombinant DNA molecule, a protein having the amino acid sequence encoded by said gene, an enzyme which is a multimer of said protein, and a process for preparing said protein or enzyme are provided, said cephalosporin acetylhydrolase being an enzyme useful for converting cephalosporins such as cephalosporin C and 7-ACA into deacetylated ones such as deacetylcephalosporin C and deacetyl 7-ACA which are useful as an intermediate for preparing a variety of derivatized cephalosporin antibiotics.
    Type: Grant
    Filed: April 22, 1991
    Date of Patent: January 25, 1994
    Assignee: Shionogi & Co., Ltd.
    Inventors: Kenji Mitsushima, Akio Takimoto, Shigeo Yagi, Takayasu Sonoyama