Abstract: The present invention addresses the problem of providing a novel method which is for preparing a DNA fragment for microbial cell transformation, and by which the combinatorial library of a long-chain DNA can be efficiently constructed and confirmation of the genotype of the obtained clone is facilitated.

Abstract: The present invention addresses the problem of providing a novel method which is for preparing a DNA fragment for microbial cell transformation, and by which the combinatorial library of a long-chain DNA can be efficiently constructed and confirmation of the genotype of the obtained clone is facilitated.

Abstract: Provided are: a method for preparing a DNA unit composition in which the mol number of a plurality of DNA units is more uniform, and a method for creating concatenated DNA. The method for preparing a DNA unit composition has: a step for preparing solutions which contain a plurality of DNA units to which an added sequence is linked, and preparing a solution for each type of DNA unit and a step for, after preparing each of the solutions, measuring the concentration of the DNA unit in each of the solutions in a state where the added sequence is linked to the DNA unit, and on the basis of the results thereof, fractionating each of the solutions and making the mol number of the DNA unit in each of the solutions closer to being identical to one another.

Abstract: The present invention addresses the problem of providing a novel method which is for preparing a DNA fragment for microbial cell transformation, and by which the combinatorial library of a long-chain DNA can be efficiently constructed and confirmation of the genotype of the obtained clone is facilitated.

Abstract: Provided are: a method for preparing a DNA unit composition in which the mol number of a plurality of DNA units is more uniform, and a method for creating concatenated DNA. The method for preparing a DNA unit composition has: a step for preparing solutions which contain a plurality of DNA units to which an added sequence is linked, and preparing a solution for each type of DNA unit and a step for, after preparing each of the solutions, measuring the concentration of the DNA unit in each of the solutions in a state where the added sequence is linked to the DNA unit, and on the basis of the results thereof, fractionating each of the solutions and making the mol number of the DNA unit in each of the solutions closer to being identical to one another.

Abstract: A method for making a recombinant gene includes searching a database using a nucleotide sequence of a coding region, a nucleotide sequence that encodes an amino acid sequence, or an amino acid sequence, of a gene, for one or more nucleotide sequences having homology; selecting one or more nucleotide sequences other than nucleotide sequences only derived from a genome from the selected nucleotide sequences; for ones of the selected one or more nucleotide sequences comprising an upstream or downstream nucleotide sequence, analyzing whether the upstream or downstream nucleotide sequence is a functional sequence to select one or more first functional sequences; for ones of the selected one or more nucleotide sequences comprising no upstream or downstream nucleotide sequence, analyzing whether a gene information has any description indicating a functional sequence to select one or more second functional sequences; scoring the selected functional sequences; and selecting one or more functional sequences.

Abstract: Provided are: a method for preparing a DNA unit composition in which the mol number of a plurality of DNA units is more uniform, and a method for creating concatenated DNA. The method for preparing a DNA unit composition has: a step for preparing solutions which contain a plurality of DNA units to which an added sequence is linked, and preparing a solution for each type of DNA unit; and a step for, after preparing each of the solutions, measuring the concentration of the DNA unit in each of the solutions in a state where the added sequence is linked to the DNA unit, and on the basis of the results thereof, fractionating each of the solutions and making the mol number of the DNA unit in each of the solutions closer to being identical to one another.

Abstract: The object of the present invention is to provide a method for synthesizing a double-stranded DNA fragment using a PCR method, the method being capable of accurately, easily, efficiently, and quickly synthesizing a double-stranded DNA fragment of interest, regardless of the sequence thereof. The present invention is a method for synthesizing a double-stranded DNA by connecting short double-stranded DNAs to each other by overlap extension PCR to obtain a double-stranded DNA fragment of interest, the method having a plurality of stages of annealing temperature setting in a PCR cycle of the overlap extension PCR.

Abstract: A surface treatment agent and a surface treatment method capable of imparting excellent corrosion resistance while enhancing adhesion between a surface of a metal substrate and a resin-containing layer. The surface treatment agent for treating a surface of a metal substrate includes an acrylic resin having a carboxyl group and a hydroxyl group, an oxazoline group-containing compound and metal particles containing at least one of Nb, Ca and Nd. The metal may be in the form of a niobium oxide sol.

Abstract: Provided are: a method for preparing a DNA unit composition in which the mol number of a plurality of DNA units is more uniform, and a method for creating concatenated DNA. The method for preparing a DNA unit composition has: a step for preparing solutions which contain a plurality of DNA units to which an added sequence is linked, and preparing a solution for each type of DNA unit; and a step for, after preparing each of the solutions, measuring the concentration of the DNA unit in each of the solutions in a state where the added sequence is linked to the DNA unit, and on the basis of the results thereof, fractionating each of the solutions and making the mol number of the DNA unit in each of the solutions closer to being identical to one another.

Abstract: A surface treatment agent and a surface treatment method capable of imparting excellent corrosion resistance while enhancing adhesion between a surface of a metal substrate and a resin-containing layer. The surface treatment agent for treating a surface of a metal substrate includes an acrylic resin having a carboxyl group and a hydroxyl group, an oxazoline group-containing compound and metal particles containing at least one of Nb, Ca and Nd. The metal may be in the form of a niobium oxide sol.

Abstract: A storage control apparatus configured to control a storage in a storage apparatus includes memory which stores assignment information that includes an assignment state of memory areas of the storage; and a processor which obtains performance information that indicates response performance for accessing the storage, and controls based on the obtained performance information, execution of an initialization process for a memory area in the storage, identified by the assignment information, and subject to initialization.

Abstract: A surface treatment agent for aluminum heat exchangers, which includes a zirconium element, vanadium element, fluorine element, aluminum element and an acrylic polymer, with the concentration of zirconium element in terms of zirconium being 100-100,000 ppm by mass, the concentration of vanadium element in terms of vanadium being 50-100,000 ppm by mass, the fluorine element concentration being 125-125,000 ppm by mass, the concentration of aluminum element in terms of aluminum being 5-10,000 ppm by mass and the concentration of the acrylic polymer being 100-100,000 ppm by mass. The surface treatment agent has a pH of 0.5-3, and suppresses odor generated from an aluminum heat exchanger, and the generation of white rust that deposits on the surface of an aluminum fin.

Abstract: An apparatus for detecting deterioration of a lightning arrester includes: a high-frequency power supply; a pair of detecting terminals 12 connected to the high-frequency power supply via a resistance for measurement and touched to the terminals of the arrester; a voltage detecting circuit for obtaining a terminal voltage of the resistance for measurement when the detecting terminals touch the terminals of the arrester; and a determining circuit for comparing the detected voltage with a reference value to determine whether the detected voltage is good or bad. A voltage having a frequency sufficiently higher than the operation frequency of a power line is generated by the high-frequency power supply and applied to the arrester, and change in electrical characteristics due to deterioration of zinc oxide elements is detected as change in current including capacitive current, and then it is determined whether the detected change is good or bad.

Abstract: An apparatus for detecting deterioration of a lightning arrester includes: a high-frequency power supply; a pair of detecting terminals 12 connected to the high-frequency power supply via a resistance for measurement and touched to the terminals of the arrester; a voltage detecting circuit for obtaining a terminal voltage of the resistance for measurement when the detecting terminals touch the terminals of the arrester; and a determining circuit for comparing the detected voltage with a reference value to determine whether the detected voltage is good or bad. A voltage having a frequency sufficiently higher than the operation frequency of a power line is generated by the high-frequency power supply and applied to the arrester, and change in electrical characteristics due to deterioration of zinc oxide elements is detected as change in current including capacitive current, and then it is determined whether the detected change is good or bad.

Abstract: A unitary seat assembly for mounting a pair of seats on a bicycle includes a central longitudinal bar portion and a first and second vertical bar portion having different lengths which extend from the central bar portion. Bicycle seats are mounted on the ends of said first and second bar portions, one of said seats being above the pedals of the bicycle while the second seat is positioned either behind or in front of the first seat. The first seat is set at a height to permit the full downward extension of the rider's legs when pedaling while the second seat is set at a height low enough to permit the rider's legs to comfortably reach the ground.

Abstract: A unitary seat assembly for mounting a pair of seats on a bicycle includes a central longitudinal bar portion and a first and second vertical bar portion having different lengths which extend from the central bar portion. Bicycle seats are mounted on the ends of said first and second bar portions, one of said seats being above the pedals of the bicycle while the second seat is positioned either behind or in front of the first seat. The first seat is set at a height to permit the full downward extension of the riders legs when pedaling while the second seat is set at a height low enough to permit the rider's legs to comfortably reach the ground. The higher of the two seats may be pivotally mounted to facilitate moving to the lower seat.

Abstract: The present invention is to provide a metal surface-treating method which is capable of forming a zinc phosphate coat suitable for the cationic electrodeposition coating of a metallic shaped product, particularly a metallic shaped product having both an iron type metallic surface and a zinc type metallic surface and is suited to a closed system.

Abstract: This invention provides a metal surface-treating method which comprises a chemical conversion step of dipping a substrate in an acidic aqueous zinc phosphate solution, and using an aqueous zinc nitrite solution as an accelerator, said aqueous zinc nitrite solution being substantially free of calcium ion and containing 0 to 6500 ppm of sodium ion and 0 to 20 ppm of sulfate ion in case of assuming the concentration of zinc nitrite [Zn(NO2)2] therein to be 10 weight % as NO2.

Abstract: A unitary seat assembly for mounting a pair of seats on a bicycle including a central longitudinal bar portion and a first and second vertical bar portion having different lengths which extend from the central bar portion. Bicycle seats are mounted on the ends of said first and second bar portions, one of said seats being above the pedals of the bicycle while the second seat is positioned either behind or in front of the first seat. The first seat is set at a height to permit the full downward extension of the riders legs when pedaling while the second seat is set at a height low enough to permit the rider's legs to comfortably reach the ground. The higher of the two seats may be pivotally mounted to facilitate moving to the lower seat.