Abstract: Methods for the recovery of nucleic acids from a nucleic acid-containing material are provided, by which nucleic acids can be rapidly and easily recovered at a high purity without deteriorating the yield. The methods are composed of step 1 for promoting the release of nucleic acids from a nucleic acid-containing material, step 2 for mixing the released nucleic acids with an accelerator substance for the binding of nucleic acids to a solid phase, step 3 for making the mixture in contact with a solid phase bondable to nucleic acids, step 4 for isolating the solid phase from a liquid, step 6 for washing the solid phase with a solution containing a salt, and a step 6 for eluting the nucleic acids from the solid phase. Accordingly, nucleic acids at a suitable purity for genetic tests or gene analyses can be rapidly and easily recovered without the use of hazardous substances.

Abstract: There are beforehand prepared a monomer having a reaction residue and a polynucleotide probe set comprising plural kinds of polynucleotide probes having a residue bonded to the reaction residue. The monomer is mixed with each kind of polynucleotide probes comprising any plural probes selected from the polynucleotide probe set. Each kind of the resultant mixtures is added to each of different small holes to make the mixture into gel matrix. Thus, a polynucleotide probe chip is produced. Sample DNA is forcibly migrated in the gels by electrophoresis. Laser light is projected onto the side face of the chip. The fluorescence emitted from the whole surface of the chip is collectively detected with a high-sensitive two-dimensional detector. Thus, the polynucleotide probe chip, holding various kinds of DNA probes, for detecting DNA can be provided. This chip has high hybridization-efficiency and makes high-sensitivity and high-speed DNA detection possible.

Abstract: Methods and apparatus are provided for the recovery of nucleic acids from a nucleic acid-containing material by which nucleic acids can be rapidly and easily recovered at a high purity without deteriorating the yield. The methods are composed of step 1 for promoting the release of nucleic acids from a nucleic acid-containing material, step 2 for mixing the released nucleic acids with an accelerator substance for the binding of nucleic acids to a solid phase, step 3 for bringing the mixture in contact with a solid phase bondable to nucleic acids, step 4 for isolating the solid phase from a liquid, step 5 for washing the solid phase with a solution containing a salt, and a step 6 for eluting the nucleic acids from the solid phase. Accordingly, nucleic acids at a suitable purity for genetic tests or gene analyses can be rapidly and easily recovered without the use of hazardous substances.

Abstract: A DNA sample added with a plurality of fluorescent marks is irradiated with excitation lights having a plurality of wavelengths and when fluorescence intensities are detected separately, the excitation light and a position on the sample are relatively changed over a desired area once to thereby detect the plurality of fluorescent marks at high speed.

Abstract: Processing of applying ultraviolet rays to a front face of an insulating film material formed on a wafer W is performed, whereby a contact angle of the front face thereof becomes smaller. Accordingly, when an insulating film material is applied on the aforesaid front face, the material smoothly spreads, and projections and depressions never occur on a front face of an upper layer insulating film material. Thereby, it is possible to form the insulating film thick and flatter on a substrate.

Abstract: Disclosed is a test chamber capable of performing a stain step without moving the test chamber itself under a microscope. The test chamber for observing and testing a smeared specimen, with cells and a tissue specimen comprises an observation portion (fixing portion) for observing the specimen and reagent storage portions, each storing a different reagent (stain liquid, rinse liquid) for stain from others therein, and sends the reagent to the specimen in response to stimulation from the outside to perform the stain.

Abstract: A sample and a binding enhancer are supplied to a treating container using a pipette tip connected to a connecting nozzle. A mixture in the treating container is sucked into a nucleic acid trapping pipette tip, and nucleic acid in the mixture is trapped by a solid phase substance contained in the pipette tip. After the solid phase substance is washed with a washing solution, an eluting solution is sucked into the nucleic acid trapping pipette tip, and a liquid containing the eluted nucleic acid is discharged to a purified product container.

Abstract: Different probes each having a specific base sequence are immobilized to each of independent areas formed on the surface of a substrate, complementary polynucleotides in a sample solution are hybridized to the probes, and each of the independent areas on the substrate is heated and then cooled in sequence, and hence the solution is recovered to extract different polynucleotides separately corresponding to individual probes.

Abstract: Processing of applying ultraviolet rays to a front face of an insulating film material formed on a wafer W is performed, whereby a contact angle of the front face thereof becomes smaller. Accordingly, when an insulating film material is applied on the aforesaid front face, the material smoothly spreads, and projections and depressions never occur on a front face of an upper layer insulating film material. Thereby, it is possible to form the insulating film thick and flatter on a substrate.

Abstract: Different probes each having a specific base sequence are immobilized to each of independent areas formed on the surface of a substrate, complementary polynucleotides in a sample solution are hybridized to the probes, and each of the independent areas on the substrate is heated and then cooled in sequence, and hence the solution is recovered to extract different polynucleotides separately corresponding to individual probes.

Abstract: Different probes each having a specific base sequence are immobilized to each of independent areas formed on the surface of a substrate, complementary polynucleotides in a sample solution are hybridized to the probes, and each of the independent areas on the substrate is heated and then cooled in sequence, and hence the solution is recovered to extract different polynucleotides separately corresponding to individual probes.

Abstract: Different probes each having a specific base sequence are immobilized to each of independent areas formed on the surface of a substrate, complementary polynucleotides in a sample solution are hybridized to the probes, and each of the independent areas on the substrate is heated and then cooled in sequence, and hence the solution is recovered to extract different polynucleotides separately corresponding to individual probes.

Abstract: Different probes each having a specific base sequence are immobilized to each of independent areas formed on the surface of a substrate, complementary polynucleotides in a sample solution are hybridized to the probes, and each of the independent areas on the substrate is heated and then cooled in sequence, and hence the solution is recovered to extract different polynucleotides separately corresponding to individual probes.

Abstract: A sample and a binding enhancer are supplied to a treating container using a pipette tip connected to a connecting nozzle. A mixture in the treating container is sucked into a nucleic acid trapping pipette tip, and nucleic acid in the mixture is trapped by a solid phase substance contained in the pipette tip. After the solid phase substance is washed with a washing solution, an eluting solution is sucked into the nucleic acid trapping pipette tip, and a liquid containing the eluted nucleic acid is discharged to a purified product container.

Abstract: Different probes each having a specific base sequence are immobilized to each of independent areas formed on the surface of a substrate, complementary polynucleotides in a sample solution are hybridized to the probes, and each of the independent areas on the substrate is heated and then cooled in sequence, and hence the solution is recovered to extract different polynucleotides separately corresponding to individual probes.

Abstract: A device comprises a means for radiating ultrasound from one face of a chamber holding a sample solution containing particles to be concentrated, and a reflective face inclined to the face irradiated with the ultrasound. The frequency of the ultrasound radiated from the ultrasound radiating means is changed periodically and asymmetrically with the passage of time.

Abstract: A rectangular-shaped holder containing sample solution is moved within a temperature adjusting box by a feeder. Then, the holder is moved by another feeder to the direction substantially perpendicular to the longitudinal direction of the holder by a length equal to the width of the single holder. The holders thus sequentially fed within the temperature adjusting box are disposed within the box in a closely contacted state from one another. There are three holders within the temperature adjusting box in advance and four holders are stayed within the box. Rectangular-shaped four heaters are disposed within the temperature adjusting box such that the heating temperature thereof is controlled by a temperature adjusting controller. A compensation heater controlled by a temperature compensating controller compensates for a heat quantity shifted to the holder having been newly housed within the temperature adjusting box from the holders already housed within the temperature adjusting box.

Abstract: A stirrer for mixing a sample solution. In order to make a structure wherein drops are not liable to remain in a channel without an increase in flow resistance in a microtube, ultrasonic vibrators are arranged in the stirring tube and plural sample solutions to be mixed are stirred and mixed by an acoustic streaming from ultrasounds generated by the vibrators.

Abstract: A cell separation module for separating and collecting cells having a charge and cells having no charge comprises a holder and a throwaway cell separation chamber held by the holder. Ultrasound is introduced into the cell separation chamber so as to allow force for causing cells to be put together to act into the cell separation chamber. In addition, an electrostatic force is allowed to act in the cell separation chamber so as to cause the cell to move by the charges of the cells. Competition between both the forces is used to separate the cells correspondingly to the freshness of the cells.

Abstract: Different probes each having a specific base sequence are immobilized to each of independent areas formed on the surface of a substrate, complementary polynucleotides in a sample solution are hybridized to the probes, and each of the independent areas on the substrate is heated and then cooled in sequence, and hence the solution is recovered to extract different polynucleotides separately corresponding to individual probes.