Abstract: A reduction in wake effects in large wind farms through wake-aware control can improve farm efficiency. The wake of a wind turbine presents complications for nearby turbines, depending on the atmospheric conditions, turbine characteristics, and turbine siting. The nascent field of wind farm flow control seeks to reduce the deleterious effects of the wake momentum deficit by leveraging the turbine as a flow actuator though the intelligent scheduling of either the blade pitch, rotor speed, or nacelle yaw.

Abstract: Methods, compositions and kits for quantitatively determining specified amounts of 11dhTxB2 in microliter to milliliter quantities of a given sample, wherein, the sample is a biological fluid. Specifically, the biological fluid is a quantity of 1 ml or less of urine from a human subject. The methods may be in the form of consolidated assays that can be run in a high throughput, automation format, such as an enzyme-linked immunosorbent assay (ELISA). Further, the ELISA may be modified into a chemiluminescence assay in order to increase sensitivity and linear range and to reduce the reaction time.

Abstract: Disclosed are compositions and methods for making differentiable amplicon species at unequal ratios using a single amplification system in a single vessel. The number of differentiable amplicons and their ratios to one another are chosen to span the required linear dynamic range for the amplification reaction and to accommodate limitations of the measuring system used to determine the amount of amplicon generated. Unequal amounts of distinguishable amplicon species are generated by providing unequal amounts of one or more amplification reaction components (e.g., distinguishable amplification oligomers, natural and unnatural NTP in an NTP mix, or the like). The amount of target nucleic acid present in a test sample is determined using the linear detection range generated from detection of one or more amplicon species having an amount within the dynamic range of detection.

Abstract: Disclosed are compositions and methods for making differentiable amplicon species at unequal ratios using a single amplification system in a single vessel. The number of differentiable amplicons and their ratios to one another are chosen to span the required linear dynamic range for the amplification reaction and to accommodate limitations of the measuring system used to determine the amount of amplicon generated. Unequal amounts of distinguishable amplicon species are generated by providing unequal amounts of one or more amplification reaction components (e.g., distinguishable amplification oligomers, natural and unnatural NTP in an NTP mix, or the like). The amount of target nucleic acid present in a test sample is determined using the linear detection range generated from detection of one or more amplicon species having an amount within the dynamic range of detection.

Abstract: The present invention relates to reagents for use in deactivating nucleic acids and methods of making and using the same.

Abstract: Disclosed are compositions and methods for making differentiable amplicon species at unequal ratios using a single amplification system in a single vessel. The number of differentiable amplicons and their ratios to one another are chosen to span the required linear dynamic range for the amplification reaction and to accommodate limitations of the measuring system used to determine the amount of amplicon generated. Unequal amounts of distinguishable amplicon species are generated by providing unequal amounts of one or more amplification reaction components (e.g., distinguishable amplification oligomers, natural and unnatural NTP in an NTP mix, or the like). The amount of target nucleic acid present in a test sample is determined using the linear detection range generated from detection of one or more amplicon species having an amount within the dynamic range of detection.

Abstract: Disclosed are compositions and methods for making differentiable amplicon species at unequal ratios using a single amplification system in a single vessel. The number of differentiable amplicons and their ratios to one another are chosen to span the required linear dynamic range for the amplification reaction and to accommodate limitations of the measuring system used to determine the amount of amplicon generated. Unequal amounts of distinguishable amplicon species are generated by providing unequal amounts of one or more amplification reaction components (e.g., distinguishable amplification oligomers, natural and unnatural NTP in an NTP mix, or the like). The amount of target nucleic acid present in a test sample is determined using the linear detection range generated from detection of one or more amplicon species having an amount within the dynamic range of detection.

Abstract: Disclosed are compositions and methods for making differentiable amplicon species at unequal ratios using a single amplification system in a single vessel. The number of differentiable amplicons and their ratios to one another are chosen to span the required linear dynamic range for the amplification reaction and to accommodate limitations of the measuring system used to determine the amount of amplicon generated. Unequal amounts of distinguishable amplicon species are generated by providing unequal amounts of one or more amplification reaction components (e.g., distinguishable amplification oligomers, natural and unnatural NTP in an NTP mix, or the like). The amount of target nucleic acid present in a test sample is determined using the linear detection range generated from detection of one or more amplicon species having an amount within the dynamic range of detection.

Abstract: The present invention relates to reagents for use in deactivating nucleic acids and methods of making and using the same.

Abstract: Disclosed are compositions and methods for making differentiable amplicon species at unequal ratios using a single amplification system in a single vessel. The number of differentiable amplicons and their ratios to one another are chosen to span the required linear dynamic range for the amplification reaction and to accommodate limitations of the measuring system used to determine the amount of amplicon generated. Unequal amounts of distinguishable amplicon species are generated by providing unequal amounts of one or more amplification reaction components (e.g., distinguishable amplification oligomers, natural and unnatural NTP in an NTP mix, or the like). The amount of target nucleic acid present in a test sample is determined using the linear detection range generated from detection of one or more amplicon species having an amount within the dynamic range of detection.

Abstract: Disclosed are compositions and methods for making differentiable amplicon species at unequal ratios using a single amplification system in a single vessel. The number of differentiable amplicons and their ratios to one another are chosen to span the required linear dynamic range for the amplification reaction and to accommodate limitations of the measuring system used to determine the amount of amplicon generated. Unequal amounts of distinguishable amplicon species are generated by providing unequal amounts of one or more amplification reaction components (e.g., distinguishable amplification oligomers, natural and unnatural NTP in an NTP mix, or the like). The amount of target nucleic acid present in a test sample is determined using the linear detection range generated from detection of one or more amplicon species having an amount within the dynamic range of detection.

Abstract: Disclosed are compositions and methods for making differentiable amplicon species at unequal ratios using a single amplification system in a single vessel. The number of differentiable amplicons and their ratios to one another are chosen to span the required linear dynamic range for the amplification reaction and to accommodate limitations of the measuring system used to determine the amount of amplicon generated. Unequal amounts of distinguishable amplicon species are generated by providing unequal amounts of one or more amplification reaction components (e.g., distinguishable amplification oligomers, natural and unnatural NTP in an NTP mix, or the like). The amount of target nucleic acid present in a test sample is determined using the linear detection range generated from detection of one or more amplicon species having an amount within the dynamic range of detection.

Abstract: A rotational sensing system is disclosed that includes a first sensing device including a first portion fixed to a body to rotate therewith in unison and provide a plurality of first device signals, a second sensing device including a second portion mechanically coupled to the body to rotate with a mechanical turn ratio relative to the body and provide a plurality of second device signals, and signal processing circuitry responsive to the first device signals to represent rotation of the first portion relative to the body with a virtual turn ratio different than unity and the mechanical turn ratio. This signal processing circuitry also includes logic operable to provide an output representative of rotational position of the body over an angular range spanning more than 360° as a function of the first device signals and the second device signals based on the virtual turn ratio.

Abstract: Compositions, methods and devices for detecting nucleic acids. The invention particularly regards composite arrays of immobilized amplification primers and hybridization probes. Also disclosed are compositions and methods for covalently immobilizing oligonucleotides and other biological molecules to glass and plastic surfaces.

Abstract: A method of sensing absolute position of a structure includes: generating a signal pattern to repetitively provide a changing voltage to each of two or more tracks of a sensor, capacitively coupling an electrode of the sensor to the tracks to determine a first electrode position along the tracks by detecting a first group of signals emitted in response to the signal pattern, moving at least one of the electrode and the tracks relative to another of the electrode and the tracks to result in a second electrode position along the tracks different from the first electrode position, and detecting a second group of signals emitted in response to the signal pattern with the electrode capacitively coupled to the tracks to determine the second electrode position.

Abstract: One embodiment of the present invention comprises a sensor with a face having several tracks spaced apart from one another. One of these tracks has a first electrode and a second electrode separated by an electrically nonconductive gap. Also included is a detection device extending across the tracks to receive signals by capacitive coupling. Sensor circuitry electrically coupled to the tracks and the detection device is structured to generate a first number of bits from a sequential signal pattern applied to the tracks in accordance with an established sequence. The circuitry is also structured to generate a second number of bits as a function of a first signal and a second signal. The first bits and second bits represent a position of the detection device along the tracks with the first bits being more numerically significant than the second bits.

Abstract: One embodiment of the present invention includes a sensor with a face having several tracks spaced apart from one another. One of these tracks has a first electrode and a second electrode separated by an electrically nonconductive gap. Also included is a detection device extending across the tracks to receive signals by capacitive coupling. Sensor circuitry electrically coupled to the tracks and the detection device is structured to generate a first number of bits from a sequential signal pattern applied to the tracks in accordance with an established sequence. The circuitry is also structured to generate a second number of bits as a function of a first signal and a second signal. The first bits and second bits represent a position of the detection device along the tracks with the first bits being more numerically significant than the second bits.

Abstract: One embodiment of the present invention comprises a sensor with a face having several tracks spaced apart from one another. One of these tracks has a first electrode and a second electrode separated by an electrically nonconductive gap. Also included is a detection device extending across the tracks to receive signals by capacitive coupling. Sensor circuitry electrically coupled to the tracks and the detection device is structured to generate a first number of bits from a sequential signal pattern applied to the tracks in accordance with an established sequence. The circuitry is also structured to generate a second number of bits as a function of a first signal and a second signal. The first bits and second bits represent a position of the detection device along the tracks with the first bits being more numerically significant than the second bits.

Abstract: Unitary hybridization probes having stem-and-loop structures, wherein the stem portion of the structure comprises a pair of interactive arms that are substantially prevented from interacting with target polynucleotides. One arm of the invented parallel-stem hybridization probe has a backbone polarity opposite that of the target-complementary loop sequence of the probe. Rather than interacting in an antiparallel fashion, the arms of parallel-stem hybridization probes interact in a parallel fashion. The arms of the invented dual inversion probes interact in a conventional antiparallel fashion, but have backbone polarities opposite that of the target-complementary loop portion of the probe. Arm portions of the inversion probes do not substantially contribute to sequence-dependent stabilization of probe:target hybrids. Incorporating inversion linkages into the structures of these probes dramatically simplifies the process of designing stem-and-loop hybridization probes.

Abstract: A process of fragmenting and labeling a synthetic or natural nucleic acid, comprising the steps of providing a mixture containing a nucleic acid, a labeling agent containing a detectable label, and at least one multivalent metal cation in a substantially aqueous solution; chemically fragmenting the nucleic acid in the mixture to produce a multiplicity of nucleic acid fragments; and attaching at least one label to at least one of the nucleic acid fragments to produce a detectably labeled nucleic acid fragment.