Abstract: The present invention relates generally to methods for genetic modification of hematopoietic cells. In particular, the invention relates to use of Prostaglandin E2 (PGE2), poloxamer, and protamine sulfate to enhance transduction by a recombinant retroviral vector. The compositions and methods of the present disclosure are particularly suitable for gene therapy applications, including the treatment of monogenic genetic diseases and disorders.

Abstract: The present disclosure provides methods for manufacturing a recombinant lentiviral vectors in an adherent bioreactor, for example, by calcium-phosphate transfection of cells grown in adherent mode on low-compaction macrocarriers in an iCELLis® bioreactor system.

Abstract: In one embodiment, a method for applying body modifications to an avatar in a virtual world environment is defined. The method includes an operation to detect movement of the avatar into a facility that is a virtual world representation of a body modification facility. In another operation the method requests submission of a body modification to be applied to the avatar. The method also includes an operation to receive the submission of the body modification where the submission is a graphic illustration of the body modification. The method sends the submission of the body modification to a review process in order to monitor body modifications within the virtual world environment. In another operation, the method applies the body modification to the avatar once the body modification has passed the review process.

Abstract: A carrier vehicle for transporting disabled cars has a support platform including a support deck between a pair of side boards, the deck being formed from a number of extruded aluminum panels and the side boards each being an aluminum extrusion. The panels are interconnected together by integral locking connectors at corresponding ends and have feet at the end of downwardly depending legs which are supported at the lateral sides by lips on the side boards which together with an upper lip forms a recess for receiving the sides of the deck panels. The construction interconnects the panels together and with the side boards to limit or preclude relative vertical movement thereby providing a strong load bearing structure and minimizing the number of welds required.

Abstract: A carrier vehicle for carrying at least one car or the like on a platform slidable and tiltable relative to a fixed bed includes hold-down devices for locking the platform against tilting when the platform is in a rest position in the forward end of the bed. Hydraulic cylinders selectively slide the platform and tilt the platform by separate control rods which are manually actuated. The vehicle includes a safety device for preventing tilting of the platform when the platform is locked by the hold-down devices so as not to break the hold-down devices and for preventing the platform from tilting downwardly onto the hold-down devices when the platform has been slid forwardly but not tilted completely for locking by the hold-down devices. The safety device includes a cam plate fixed to the control rod which effects tilting and a stop plate fastened to a rail forming a portion of the platform.