Abstract: In one example in accordance with the present disclosure, a fluid ejection controller is described. The fluid ejection controller includes a firing board to pass control signals to a fluid ejection device to eject fluid from the fluid ejection device. A mount pivotally holds the firing board between a disengaged position where electrical pins of the firing board are not in contact with electrical pads of the fluid ejection device and an engaged position where the electrical pins are in contact with the electrical pads. The mount includes a slot to receive the fluid ejection device and at least one biasing spring to bias the firing board away from the fluid ejection device during insertion of the fluid ejection device. The fluid ejection controller also includes a handle coupled to a cam shaft to move the firing board between the disengaged position and the engaged position.

Abstract: Methods and devices useful for determining the analyte concentration of a sample using output currents obtained from an input potential in the kinetic potential region of a redox mediator are disclosed. Preferably, the input potential used to generate the output currents from the kinetic potential region of the redox mediator is continually increasing with time after initiating the analysis. A method of selecting an initial input potential within the kinetic potential region of a redox mediator based on the sensitivity of an individual or batch of subcutaneously insertable test sensors also is described. A method of selecting an analysis input potential within the kinetic potential region of redox mediator based on the sensitivity of an individual subcutaneously inserted test sensor also is described where the analysis input potential is increased with insertion time.

Abstract: An apparatus for measuring a characteristic of a sample using a centrifuge and optical components is disclosed. The centrifuge may be a standard benchtop centrifuge. The optical components may be sized and dimensioned to fit, along with the sample, inside the centrifuge.

Abstract: Described herein are systems and methods for creating a transport tube comprising a plurality of passages configured to deliver an composition from a source into the human subcutaneous space.

Abstract: Described herein are systems and methods for sensing an analyte concentration and delivering a composition to a subcutaneous space of a subject comprising a sensing cannula, which comprises an electrode for detecting the analyte concentration in the subcutaneous space.

Abstract: A fluid ejection system, in an example, includes at least one nozzle of at least one die from which a fluid is ejected and at least one nanowell at which the at least one nozzle ejects an amount of fluid. A method of dispensing a fluid, in an example, includes addressing at least one nanowell with at least one nozzle of at least one die, and depositing a fluid in the nanowell with the at least one nozzle.

Abstract: A device for delivery of an insulin or insulin analog formulation and measurement of subcutaneous glucose concentration may comprise a hollow tube, and an amperometric glucose sensor located proximal to a distal end of the hollow tube. The amperometric glucose sensor may comprise a redox mediator and an enzyme comprising glucose oxidase or glucose dehydrogenase. An applied bias potential may allow an electrode layer of the amperometric glucose sensor to undergo substantially no electropolymerization of an excipient of the insulin or insulin analog formulation during continuous operation of amperometric glucose sensor. A sensitivity of the amperometric glucose sensor to the subcutaneous glucose concentration may be maintained in presence of the insulin or insulin analog formulation.

Abstract: By combining analyte concentration monitoring electrodes and infusion functions into a single subcutaneous element, the described sensing cannulae having a rectangular cross-section avoids the need for two separate devices for insulin delivery and glucose concentration determination. The substantially flat, thus planar, surface of the sensing cannula provides a substrate for deposition of one or more sensing electrodes, preferably through a lithographic-type process. The inner lumen of the sensing cannula serves as a conduit for drug delivery and is formed in a manner that is compatible with lithographic-type electrode formation. The rectangular cross-section of the sensing cannula also allows face and side ports establishing fluid communication between the inner lumen and tissue that preferably reduces the incidence of occlusion of the inner lumen of the sensing cannula.

Abstract: The present disclosure provides methods of measuring of an analyte in a subject to remove a measurement artifact by using a forecasting model to determine the true analyst concentration in a subject. Also herein, the present disclosure provides parameters and models to estimate the true analyte concentration in a subject.

Abstract: The present disclosure provides systems and methods for managing blood glucose in a subject. An insulin delivery cannula may comprise a hollow tube comprising proximal and distal ends, the proximal end in fluid communication with an insulin source, and the distal end configured to deliver insulin into a subcutaneous space. An insulin infusion pump may be fluidically coupled to the proximal end, and attached to the insulin delivery cannula directly or via an intervening tube. The glucose sensor and the insulin delivery cannula may be configured to be inserted into the subcutaneous space simultaneously by a single insertion device. A lumen of the insulin delivery cannula may be contiguous with a lumen of tubing from the insulin infusion pump. Electrical conductors for the electrodes may be disposed on a wall of the tubing or attached to a surface of the tubing, and establish an electrical connection with an electronic module.

Abstract: The present disclosure provides systems and methods for managing blood glucose in a subject. An insulin delivery cannula may comprise a hollow tube comprising a proximal end and a distal end, wherein the proximal end is in fluid communication with a source of a concentrated insulin or insulin analog formulation of at least about 150 units per milliliter. The distal end may be configured to deliver the concentrated insulin or insulin analog formulation into a subcutaneous space. A continuous amperometric or coulometric glucose sensor may located no more than a pre-determined distance away from the distal end. An insulin pump may be fluidically coupled to the proximal end, and may be attached to the insulin delivery cannula directly or via an intervening tube. The glucose sensor and the insulin delivery cannula may be configured to be inserted into the subcutaneous space simultaneously by a single insertion device.

Abstract: In one example in accordance with the present disclosure, a fluid ejection controller is described. The fluid ejection controller includes a firing board to pass control signals to a fluid ejection device to eject fluid from the fluid ejection device. A mount pivotally holds the firing board between a disengaged position where electrical pins of the firing board are not in contact with electrical pads of the fluid ejection device and an engaged position where the electrical pins are in contact with the electrical pads. The mount includes a slot to receive the fluid ejection device and at least one biasing spring to bias the firing board away from the fluid ejection device during insertion of the fluid ejection device. The fluid ejection controller also includes a handle coupled to a cam shaft to move the firing board between the disengaged position and the engaged position.

Abstract: A method of manufacturing a digital dispense apparatus includes molding a monolithic carrier structure, forming cut outs into a planar top surface of the monolithic carrier structure, the cut outs including a reservoir extending into part of a thickness of the monolithic carrier structure and fluid routing to connect the reservoir with a fluid dispense device, and overmolding the fluid dispense device into the monolithic carrier structure on a side of the monolithic carrier structure that is opposite to the reservoir to fluidically connect to the fluid routing.

Abstract: The present disclosure provides systems and devices for combining analyte monitoring with fluid delivery, including devices that are adapted for use with combined sensors and cannulas having sensors and cannulas on a single component. These systems and devices may be used in various applications with simultaneous in vivo monitoring of analyte concentrations and delivery of medications.

Abstract: Disclosed herein are methods of using genetic markers associated with degenerative disc disease, for example via a computer-implemented program to predict risk of developing degenerative disc disease, and methods of preventing or treating degenerative disc disease or a symptom thereof.

Abstract: A digital dispense apparatus includes at least one fluid dispense device, at least one reservoir fluidically connected to the at least one fluid dispense device, a monolithic carrier structure carrying the at least one fluid dispense device and reservoir, the monolithic carrier forming fluid routing between the reservoir and the fluid dispense device.

Abstract: A conveyor type ware washing machine uses a heat pump to reduce exhaust heat and to heat incoming water. A modulating valve is used to control incoming water flow through a heat exchanger of the heat pump. Processing circuitry automatically dynamically adjusts the modulating valve between a plurality of open states based on the sensed refrigerant head pressure of the heat pump, so that the refrigerant head pressure is maintained in a predefined range. The ware washing machine is efficient, and reduces the amount of heat exhausted to ambient. Related methods are also disclosed.

Abstract: A microfluidic device may, in an example, include at least one microfluidic channel, a dielectrophoresis separator to separate a plurality of cells passing within the at least one microfluidic channel, and a thermal resistor to eject at least one cell from the microfluidic device. A cassette may, in an example, include a die coupled to a substrate of the cassette, the die including at least one microfluidic channel, a dielectrophoresis separator along the microfluidic channel to separate a plurality of cells passing within the microfluidic channel, and an ejection device to eject at least one of the plurality of cells into an assay well.

Abstract: A digital dispense apparatus comprising a plurality of fluid dispense devices, at least one reservoir connected to the plurality of fluid dispense devices to deliver fluid to the plurality of fluid dispense devices, at least one contact pad array, and a single monolithic carrier structure.

Abstract: A microfluidic dispenser can include a processor to receive a user input via a user interface related to limiting dilution (or a limiting dilution assay) to be performed, and calculate a dispense volume of a fluid for the limiting dilution based on the user input. The microfluidic dispenser can also include a dispense cassette including a fluid reservoir, and a microfluidic dispense head to dispense the fluid via a nozzle in accordance with the calculated dispense volume.