Abstract: A locking mechanism for releasably fixing an externally splined power take-off shaft to an output shaft of off-highway equipment such as a tractor. The output shaft has a hollow, internally splined hub portion adapted to axially receive the externally splined power take-off shaft to prevent relative rotational movement between the power take-off and output shafts. The locking mechanism includes a locking member supported within a radial opening in the power-take off shaft so as to be radially movable between an outer locking position and an inner unlocking position. In the outer locking position, the locking member is engageable with a recess formed in the hub portion of the output shaft to prevent relative axial movement between the power take-off shaft and the output shaft. In the inner unlocking position, the locking member is disengaged from the recess in the output shaft to permit such relative axial movement.