Abstract: Provided herein are reagents and kits for detection of multiple target sequences in a single-tube, single-color assay, and methods of use thereof. In particular, multiplex assays are provided for the detection of Mycobacterium tuberculosis complex target sequences (e.g., katG, rpoB, inhA promotor, pncA, etc.).

Abstract: Provided herein are reagents and kits for detection of multiple target sequences in a single-tube, single-color assay, and methods of use thereof. In particular, multiplex assays are provided for the detection of Mycobacterium tuberculosis complex target sequences (e.g., katG, rpoB, inhA promotor, pncA, etc.).

Abstract: Provided herein are reagents and kits for detection of multiple target sequences in a single-tube, single-color assay, and methods of use thereof. In particular, multiplex assays are provided for the detection of Mycobacterium tuberculosis complex target sequences (e.g., katG, rpoB, inhA promotor, pncA, etc.).

Abstract: Provided herein are mispriming prevention reagents, compositions and kits comprising such reagents and methods of use thereof.

Abstract: Provided herein are mispriming prevention reagents, compositions and kits comprising such reagents and methods of use thereof.

Abstract: The present invention relates to compositions and methods for nucleic acid based diagnostic assays. In particular, the present invention provides primers for asymmetric PCR and other amplification modalities. In some embodiments, the present invention provides multiple primers for amplification of related nucleic acid targets in a single reaction.

Abstract: Homogenous detection during or following PCR amplification, preferably LATE-PCR, utilizing fluorescent DNA dye and indirectly excitable labeled primers and probes, improves reproducibility and quantification. Low-temperature homogeneous detection during or following non-symmetric PCR amplification, preferably LATE-PCR, utilizing fluorescent DNA dye and indirectly excitable labeled mismatch-tolerant probes permits analysis of complex targets. Sequencing sample preparation methods following LATE-PCR amplifications reduce complexity and permit “single-tube” processing.

Abstract: Provided herein are mispriming prevention reagents, compositions and kits comprising such reagents and methods of use thereof.

Abstract: Provided herein are reagents and kits for detection of multiple target sequences in a single-tube, single-color assay, and methods of use thereof. In particular, multiplex assays are provided for the detection of Mycobacterium tuberculosis complex target sequences (e.g., katG, rpoB, inhA promotor, pncA, etc.).

Abstract: The present invention relates to compositions and methods for nucleic acid based diagnostic assays. In particular, the present invention provides primers for asymmetric PCR and other amplification modalities. In some embodiments, the present invention provides multiple primers for amplification of related nucleic acid targets in a single reaction.

Abstract: An assay comprising more than one primer pair and more than one detection probe, a low copy number synthetic amplicon corresponding to each of the primer pairs. The assay can detect and distinguish between various sub-types and strains of an influenza virus using any suitable nucleic acid amplification technique. Related kits and methods are also described.

Abstract: An assay comprising more than one primer pair and more than one detection probe, a low copy number synthetic amplicon corresponding to each of the primer pairs. The assay can detect and distinguish between various sub-types and strains of an influenza virus using any suitable nucleic acid amplification technique. Related kits and methods are also described.

Abstract: A device to prevent the gears of an automotive vehicle from being shifted from forward, reverse or neutral to another position unless the brakes are firmly applied. The transmission includes a gear shift actuator that is rotatable between forward, neutral and reverse positions, and fixed to this actuator is a lever having an axially projecting fin on one side of the free end. Pivoted on a supporting frame is a steel bar having notches in one edge that are shaped and positioned to engage the fin on the lever whenever the lever is in any one of its three operating positions. A spring bears against the bar to urge the notched edge toward the fin of the lever, so that the bar normally engages and locks the lever. A hydraulic cylinder has its piston rod attached to the bar, and the cylinder is connected to the hydraulic braking system of the vehicle, so that when the brakes are applied, the cylinder acts to disengage the bar from the lever.