Abstract: The present disclosure is directed to a nucleic acid, including: a DNA nanoswitch-nucleic acid complex including a deoxyribonucleic acid (DNA) nanoswitch and a ribonucleic acid binding site, wherein the DNA nanoswitch has a first conformation characterized as open, and a second conformation characterized as closed when in a presence of ribonucleic acid-of-interest. DNA nanoswitches that hybridize to preselected viral RNA are also disclosed, as well as methods of detecting or identifying an RNA virus, and kits related thereto.

Abstract: The present disclosure is directed to ribonucleic acid (RNA) isolation and purification. For example, the present disclosure relates to a method of purifying a single ribonucleic acid (RNA) species, including: isolating a DNA nanoswitch-target complex within a gel medium, wherein the DNA nanoswitch-target complex includes a DNA nanoswitch and a target-of-interest; digesting the DNA nanoswitch and the gel medium to form digested byproducts, and a free target-of-interest; and isolating the free target-of-interest, wherein the free target-of-interest is a single RNA species.

Abstract: A centrifuge force microscope module for use within a bucket of a centrifuge in measuring a characteristic of a sample under a centrifugal force and/or in monitoring a sample under a centrifugal force. The centrifuge force microscope module includes an electronics module and an optical module. The electronics module includes a housing removably disposable in the bucket of the centrifuge, and at least one of a power source and a connector operably connectable to a power source for powering the electronics module. The optical module is operable to receive and direct light from the sample. The optical module is releaseably connectable to the housing of the electronics module.

Abstract: A centrifuge force microscope module for use within a bucket of a centrifuge in measuring a characteristic of a sample under a centrifugal force and/or in monitoring a sample under a centrifugal force. The centrifuge force microscope module includes an electronics module and an optical module. The electronics module includes a housing removably disposable in the bucket of the centrifuge, and at least one of a power source and a connector operably connectable to a power source for powering the electronics module. The optical module is operable to receive and direct light from the sample. The optical module is releaseably connectable to the housing of the electronics module.

Abstract: A separation sampling module for use within a bucket of a centrifuge for monitoring separation of a sample in a container includes a housing operable for supporting the container for containing the sample and removably positionable within the bucket of the centrifuge, at least one light source for illuminating the sample, at least one light detector for detecting light from the sample, an accelerometer for measuring acceleration of the housing, and at least one of a power source and a connector operably connectable to a power source for use in powering the at least one light source. Light from the at least one light source passing through the sample defines a light path disposed in a direction across the direction of a centrifugal force when the separation sampling module is disposed in the bucket and rotated in the centrifuge.

Abstract: A separation sampling module for use within a bucket of a centrifuge for monitoring separation of a sample in a container includes a housing operable for supporting the container for containing the sample and removably positionable within the bucket of the centrifuge, at least one light source for illuminating the sample, at least one light detector for detecting light from the sample, and at least one of a power source and a connector operably connectable to a power source for use in powering the at least one light source. Light from the at least one light source passing through the sample defines a light path disposed in a direction across the direction of a centrifugal force when the separation sampling module is disposed in the bucket and rotated in the centrifuge.

Abstract: A centrifuge force microscope module for use within a bucket of a centrifuge in measuring a characteristic of a sample under a centrifugal force and/or in monitoring a sample under a centrifugal force. The centrifuge force microscope module includes an electronics module and an optical module. The electronics module includes a housing removably disposable in the bucket of the centrifuge, and at least one of a power source and a connector operably connectable to a power source for powering the electronics module. The optical module is operable to receive and direct light from the sample. The optical module is releaseably connectable to the housing of the electronics module.

Abstract: A spinning force system and methods of operation are provided for measuring a characteristic of a sample. The system includes a detection module having a light source for illuminating the sample and an objective being aligned to the light source to define a light path for producing an image of the sample. A rotor is mechanically coupled to the detection module and configured to rotate the light path for applying a force to the sample. The force may include a centrifugal force and other forms of force (such as a viscous drag force) resulted from the rotation. In some examples, the force is applied in a direction that is not parallel to a surface of the sample.

Abstract: A spinning force system and methods of operation are provided for measuring a characteristic of a sample. The system includes a detection module having a light source for illuminating the sample and an objective being aligned to the light source to define a light path for producing an image of the sample. A rotor is mechanically coupled to the detection module and configured to rotate the light path for applying a force to the sample. The force may include a centrifugal force and other forms of force (such as a viscous drag force) resulted from the rotation. In some examples, the force is applied in a direction that is not parallel to a surface of the sample.

Abstract: An electrode assembly for use with a catheter, is made of a plurality of insulated wires. The wires are bound together in a bundle by fine insulated wire for almost their entire lengths. At one end of the bundle are free wire ends of different lengths on which are electrically conductive ferrules. The ferrules are spaced apart axially when aligned with the bundle inside the catheter. The wires are made of springy material so that the free wire ends separate widely outside the catheter. Other cylindrical ferrules are mounted in axially spaced position on bared portions of the other end of the bundle, and encircle all the wires, but each ferrule is in direct electrical contact with only one of the wires.

Abstract: A fitting assembly for guiding and retaining a probe in a catheter, comprises a generally cylindrical housing in which is a tubular body through which a probe can pass. Inside one end of the housing is a tubular resilient gasket which bears on one end of the tubular body. An internally threaded cap is engaged on a threaded nipple at one end of the housing and is movable axially upon rotation to compress the gasket and hold the probe in place. An internal projection in the cap engages on an annular shoulder on the housing to keep the cap from being detached from the assembly. A nipple at the other end of the tubular body engages a threaded fitting at one end of the catheter. An adapter coupling is provided having an internally threaded end engageable on the threaded fitting at the end of the tubular body, and having an internally threaded other end in which is engageable an externally threaded fitting of a catheter provided with such a fitting.

Abstract: An electrode assembly for use with a catheter, is made of a plurality of insulated wires. The wires are bound together in a bundle by fine insulated wire for almost their entire lengths. At one end of the bundle are free wire ends of different lengths on which are electrically conductive ferrules. The ferrules are spaced apart axially when aligned with the bundle inside the catheter. The wires are made of springy material so that the free wire ends separate widely outside the catheter. Other cylindrical ferrules are mounted in axially spaced position on bared portions of the other end of the bundle, and encircle all the wires, but each ferrule is in direct electrical contact with only one of the wires.