Abstract: The present disclosure encompasses embodiments of a novel near-infrared-emitting molecular fluorophore and probes incorporating said fluorophore advantageous for in vitro and in vivo research studies. The fluorophore is robust, photostable, and possesses functionalities for easy bioorthogonal conjugation (e.g., click chemistry, hydrazone formation, Diels Alder, Staudinger ligation, etc.). It is biocompatible and emits at 711 nm in aqueous conditions. These fluorophores may be used to fluorescently tag biological molecules or structures of interest, or used as optical reporters (i.e., activatable molecular probes, fluorescent dyes) for specific biomarkers/analytes as they can be switched from “off” to “on.” This fluorophore is useful for cellular assays and preclinical small animal imaging as the near-infrared emission is highly penetrating, and the photophysical properties are outstanding. As such, the properties of this class of fluorophores could easily be translated for use in clinical applications.

Abstract: The invention is provides for a variety of fluorescence sensing compounds characterized generally as having a coumarin-3-aldehyde scaffold with a pendant aryl moiety at the C4-position of the scaffold. The further provides a method for detecting primary amines in a biological sample using said compounds. Especially, for the selective labeling and imaging of catecholamines in live and fixed secretory cells.

Abstract: A fluorescence sensing compound for separately detecting and visualizing one or more monoamine neurotransmitters in cells, the fluorescence sensing compound having the following formula: wherein R1 and R2 are each independently selected from the group consisting of hydrogen, alkyl, alkylene, aryl, cycloalkyl; wherein R3 is selected from the group consisting of hydrogen, alkyl, alkylene, aryl, cycloalkyl, cyano, azido; and wherein R4 is selected from the group consisting of (CH3)2Si, O, N, S, and CH2.

Abstract: The present disclosure encompasses embodiments of a novel near-infrared-emitting molecular fluorophore and probes incorporating said fluorophore advantageous for in vitro and in vivo research studies. The fluorophore is robust, photostable, and possesses functionalities for easy bioorthogonal conjugation (e.g., click chemistry, hydrazone formation, Diels Alder, Staudinger ligation, etc.). It is biocompatible and emits at 711 nm in aqueous conditions. These fluorophores may be used to fluorescently tag biological molecules or structures of interest, or used as optical reporters (i.e., activatable molecular probes, fluorescent dyes) for specific biomarkers/analytes as they can be switched from “off” to “on.” This fluorophore is useful for cellular assays and preclinical small animal imaging as the near-infrared emission is highly penetrating, and the photophysical properties are outstanding. As such, the properties of this class of fluorophores could easily be translated for use in clinical applications.

Abstract: A fluorescence sensing compound for separately detecting and visualizing one or more monoamine neurotransmitters in cells, the fluorescence sensing compound having the following formula: wherein R1 and R2 are each independently selected from the group consisting of hydrogen, alkyl, alkylene, aryl, cycloalkyl; wherein R3 is selected from the group consisting of hydrogen, alkyl, alkylene, aryl, cycloalkyl, cyano, azido; and wherein R4 is selected from the group consisting of (CH3)2Si, O, N, S, and CH2.

Abstract: The invention is provides for a variety of fluorescence sensing compounds characterized generally as having a coumarin-3-aldehyde scaffold with a pendant aryl moiety at the C4-position of the scaffold. The further provides a method for detecting primary amines in a biological sample using said compounds. Especially, for the selective labeling and imaging of catecholamines in live and fixed secretory cells.